Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Molecular Pharmacology
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Molecular Pharmacology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit molpharm on Facebook
  • Follow molpharm on Twitter
  • Follow molpharm on LinkedIn
Research ArticleArticle

Interaction of Polyene Antibiotics with Subcellular Membrane Systems I. Mitochondria

STEPHEN C. KINSKY, GARY R. GRONAU and MORTON M. WEBER
Molecular Pharmacology September 1965, 1 (2) 190-201;
STEPHEN C. KINSKY
Department of Pharmacology, Washington University School of Medicine, St. Louis, Missouri
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
GARY R. GRONAU
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
MORTON M. WEBER
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

The action of the polyene antibiotics, ascosin, candicidin, amphotericin B, candidin, etruscomycin, filipin, nystatin, and pimaricin, on Neurospora and rat liver mitochondrial succinate-cytochrome c reductase was investigated. Only the ascosin and candicidin preparations inhibited this reaction. Various criteria indicated that both these antibiotic preparations were impure. Thin-layer chromatography of an acetone extract of ascosin revealed the presence of antimycin A. The inhibition of the succinate-cytochrome c reductase by ascosin was not due to the polyene per se, but was a consequence of contamination by antimycin A.

Although mitochondrial function (electron transport, as well as oxidative phosphorylation and ion transport) is not affected by these antibiotics, Neurospora mitochondria were able to bind filipin. The antibiotic could be recovered from the mitochondria after extraction with dimethyl formamide. Previous studies have shown that the presence of sterol is a necessary prerequisite for polyene binding to cell membrances. Neurospora mitochondria contain ergosterol although less than the microsomal fraction, which is partly derived from the cell membrane. The above findings indicate that the presence of sterol may not be sufficient to confer polyene sensitivity to a membrane system. Mitochondria have a much higher phospholipid: sterol ratio than cell membranes, suggesting that the relative concentration of these lipids may be an important factor in determining polyene sensitivity.

ACKNOWLEDGMENTS This research has been supported by U.S.P.H.S. grants AI-05114 and Research Career Development Award 5-K3-AI-6388 (to SCK), and grant AI-03046 (to MMW). We are also indebted to Professor L. L. M. van Deenen, der Rijksuniversiteit, Utrecht, The Netherlands, for many helpful discussions.

  • Copyright ©, 1965, by Academic Press Inc.

MolPharm articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Molecular Pharmacology
Vol. 1, Issue 2
1 Sep 1965
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Back Matter (PDF)
  • Editorial Board (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Molecular Pharmacology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Interaction of Polyene Antibiotics with Subcellular Membrane Systems I. Mitochondria
(Your Name) has forwarded a page to you from Molecular Pharmacology
(Your Name) thought you would be interested in this article in Molecular Pharmacology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleArticle

Interaction of Polyene Antibiotics with Subcellular Membrane Systems I. Mitochondria

STEPHEN C. KINSKY, GARY R. GRONAU and MORTON M. WEBER
Molecular Pharmacology September 1, 1965, 1 (2) 190-201;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Research ArticleArticle

Interaction of Polyene Antibiotics with Subcellular Membrane Systems I. Mitochondria

STEPHEN C. KINSKY, GARY R. GRONAU and MORTON M. WEBER
Molecular Pharmacology September 1, 1965, 1 (2) 190-201;
del.icio.us logo Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Fatty acid amide hydrolase in cisplatin nephrotoxicity
  • eCB Signaling System in hiPSC-Derived Neuronal Cultures
  • Benzbromarone relaxes airway smooth muscle via BK activation
Show more Articles

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About Molecular Pharmacology
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Journal of Pharmacology and Experimental Therapeutics
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0111 (Online)

Copyright © 2023 by the American Society for Pharmacology and Experimental Therapeutics