Article Figures & Data
Figures
Additional Files
Data Supplement
- Supplemental Data 1 -
Data Supplement 1. R script for Bayesian analysis (i.e., generalized linear mixed model with group-specific terms) of OSTα/β uptake and efflux data.
- Supplemental Data 2 -
Data Supplement 2. Represents the first frame taken from the molecular dynamics (MD)-trajectory presenting structure at the time point zero, after standard relaxation protocol of the Desmond Molecular Dynamics System. Solvent, ions, and plasma membrane lipid bilayer are excluded for clarity.
- Supplemental Data 3 -
Data Supplement 3. Represents the OSTα structure at the end of the 500 ns molecular dynamics (MD) simulation. Solvent, ions, and plasma membrane lipid bilayer are excluded from the unconstrained periodic simulation for clarity.
- Supplemental Data 4 -
Data Supplement 4. The final homology model of OSTα, including OSTβ. The putative interaction with OSTβ is generated based on interactions detected in the OsSweet template structure, between chains A (OSTα) and B (OSTβ). The OSTβ structural component is used for graphical illustrations only and should be taken with caution.
- Supplemental Data -
Supplemental Table 1. OSTα forward primers used to generate OSTα/β mutants.
Supplemental Table 2. OSTα/β wild-type and OSTα/β mutant [3H]-taurocholate (TCA) transport data.
Supplemental Fig. 1. Sequence alignments of OSTα and OSTβ.
Supplemental Fig. 2. Snapshot structures of OSTα taken from a molecular dynamics (MD) trajectory at the beginning i.e., after relaxation (left panel) and after 500 ns of MD simulation (right panel).
Supplemental Fig. 3 . Simulations of the final homology model.
Supplemental Fig. 4. OSTα and OSTβ Protein Levels.
Supplemental Fig. 5. Immunocytochemistry of Mock, OSTα/β wild-type, and OSTα/β mutant cells.
Supplemental References
- Supplemental Data 1 -