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Review ArticleMinireview

Mechanisms to Repair Stalled Topoisomerase II-DNA Covalent Complexes

Rebecca L. Swan, Ian G. Cowell and Caroline A. Austin
Molecular Pharmacology January 2022, 101 (1) 24-32; DOI: https://doi.org/10.1124/molpharm.121.000374
Rebecca L. Swan
Biosciences Institute, Newcastle University, Newcastle upon Tyne, United Kingdom
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Ian G. Cowell
Biosciences Institute, Newcastle University, Newcastle upon Tyne, United Kingdom
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Caroline A. Austin
Biosciences Institute, Newcastle University, Newcastle upon Tyne, United Kingdom
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Abstract

DNA topoisomerases regulate the topological state of DNA, relaxing DNA supercoils and resolving catenanes and knots that result from biologic processes, such as transcription and replication. DNA topoisomerase II (TOP2) enzymes achieve this by binding DNA and introducing an enzyme-bridged DNA double-strand break (DSB) where each protomer of the dimeric enzyme is covalently attached to the 5′ end of the cleaved DNA via an active site tyrosine phosphodiester linkage. The enzyme then passes a second DNA duplex through the DNA break, before religation and release of the enzyme. However, this activity is potentially hazardous to the cell, as failure to complete religation leads to persistent TOP2 protein-DNA covalent complexes, which are cytotoxic. Indeed, this property of topoisomerase has been exploited in cancer therapy in the form of topoisomerase poisons which block the religation stage of the reaction cycle, leading to an accumulation of topoisomerase-DNA adducts. A number of parallel cellular processes have been identified that lead to removal of these covalent TOP2-DNA complexes, facilitating repair of the resulting protein-free DSB by standard DNA repair pathways. These pathways presumably arose to repair spontaneous stalled or poisoned TOP2-DNA complexes, but understanding their mechanisms also has implications for cancer therapy, particularly resistance to anti-cancer TOP2 poisons and the genotoxic side effects of these drugs. Here, we review recent progress in the understanding of the processing of TOP2 DNA covalent complexes, the basic components and mechanisms, as well as the additional layer of complexity posed by the post-translational modifications that modulate these pathways.

SIGNIFICANCE STATEMENT Multiple pathways have been reported for removal and repair of TOP2-DNA covalent complexes to ensure the timely and efficient repair of TOP2-DNA covalent adducts to protect the genome. Post-translational modifications, such as ubiquitination and SUMOylation, are involved in the regulation of TOP2-DNA complex repair. Small molecule inhibitors of these post-translational modifications may help to improve outcomes of TOP2 poison chemotherapy, for example by increasing TOP2 poison cytotoxicity and reducing genotoxicity, but this remains to be determined.

Introduction

Double-strand DNA breaks (DSBs) are highly lethal DNA lesions. Because of this, the generation of DSBs (for example, by radiation therapy or treatment with DSB-inducing drugs) is an effective anticancer strategy. This includes drugs targeting DNA topoisomerase II (TOP2), called TOP2 poisons, which exploit the intrinsic ability of TOP2 to induce DSBs as part of its normal reaction mechanism. Both pathways involved in the repair of DSBs, namely homologous recombination (HR) repair and non-homologous end joining (NHEJ), require “clean” DNA ends, yet the clinically relevant DSBs induced by TOP2 poisons contain blocked DNA ends covalently linked to a TOP2 protein adduct at the 5′ end. Further processing is therefore required to remove TOP2 adducts (known as TOP2-DNA covalent complexes) from DNA and produce clean, protein-free DSBs for repair. A better understanding of how TOP2-DNA covalent complexes are processed may improve therapy with TOP2 poisons by increasing cytotoxicity and reducing genotoxicity, the latter of which occurs following misrepair of TOP2 poison-induced DSBs. This review summarizes our current understanding of TOP2-DNA covalent complex repair, and how this is regulated by post-translational modifications, such as SUMOylation and ubiquitination.

DNA Topoisomerase II and TOP2 Poisons

TOP2 poisons are anticancer agents which induce cytotoxic DSBs by interfering with the normal TOP2 reaction mechanism. In the absence of a TOP2 poison, TOP2 resolves topological problems in DNA (such as supercoils, knots, and catenanes), which can otherwise inhibit many DNA-dependent processes, such as DNA replication, transcription, and chromosome segregation. This is achieved by passing one DNA strand through an enzyme-bridged DSB. The enzyme-bridged DSB is induced upon the nucleophilic attack of the phosphate backbone of DNA by the TOP2 active site tyrosine, which creates a break in the DNA strand while simultaneously forming a covalent 5′-phosphotyrosyl linkage between TOP2 and the 5′- end of DNA (Deweese and Osheroff, 2009). This intermediate of the TOP2 reaction cycle is termed the TOP2-DNA covalent complex, which facilitates strand passage through the DSB without initiating an unnecessary DNA damage response. Following strand passage, TOP2 religates the DSB in an error-free manner, and the TOP2-DNA complex is rapidly reversed as TOP2 completes its reaction cycle and dissociates from the DNA, while also concealing the break from recognition by the DNA damage response machinery. However, in the presence of a TOP2 poison, such as etoposide or mitoxantrone, religation of the TOP2-mediated DSB is inhibited, and the TOP2 protein remains covalently bound to the DNA. In contrast, TOP2 inhibitors, such as ICRF-193, inhibit the catalytic activity of TOP2 without inducing covalent complexes. TOP2-DNA covalent complexes can also be induced by endogenous events, leading to abortive TOP2 reactions. For example, alterations in DNA can cause misalignment of the broken DNA ends, thus inhibiting religation of the DSB (Sun et al., 2020). The ensuing protein-DNA adduct can stall elongating polymerases, leading to inhibition of replication and transcription and potentially cell death (D’Arpa et al., 1990). Alternatively, the TOP2-DNA covalent complex is processed to protein-free DSBs which may also lead to cell death following a canonical DNA damage response. NHEJ repair is especially important for the repair of TOP2-mediated DNA damage, as knockout of NHEJ proteins, such as KU70/80, Ligase IV or DNA-PKcs significantly increases the sensitivity of cells to TOP2-targeting agents, such as etoposide and ICRF-193, but not topoisomerase I (TOP1)-targeting agents, such as camptothecin (Adachi et al., 2003; Maede et al., 2014). In some cases, aberrant NHEJ repair leads to the generation of leukemogenic chromosome translocations (Cowell et al., 2012; Olmedo-Pelayo et al., 2020).

Drug-stabilized TOP2-DNA complexes pose a significant challenge to the DNA repair machinery, as the enzyme-linked DSB remains buried within the core of the bulky TOP2 adduct. Studies have shown that TOP2-linked DSBs themselves do not activate DNA-PK (Mårtensson et al., 2003; Muslimović et al., 2009) and thus further processing and the removal of the TOP2 protein is required before DSB repair can occur. A multitude of TOP2-DNA covalent complex processing mechanisms have been described, yet the factors influencing when or why a particular pathway is employed remain unclear. A better understanding of how these lesions are processed may help improve TOP2 poison therapy by reducing drug resistance and TOP2 poison genotoxicity.

Proteolytic Mechanisms

One potential mechanism of TOP2-DNA complex repair is the proteolytic removal of the TOP2 protein adduct, which leaves behind smaller peptide fragments which can then be removed in a further end-polishing step prior to DSB repair, such as direct cleavage by the 5′-phosphodiesterase, tyrosyl DNA phosphodiesterase 2 (TDP2). Two major proteases have been implicated in the degradation of TOP2-DNA covalent complexes, namely the proteasome and SPRTN.

Proteasomal Degradation of TOP2-DNA Covalent Complexes

Exposure to the TOP2 poisons etoposide and teniposide induces the degradation of both TOP2 isoforms (TOP2A and TOP2B) in human cells (Alchanati et al., 2009; Ban et al., 2013; Fan et al., 2008; Lee et al., 2016; Mao et al., 2001; Sunter et al., 2010). The TOP2 poison-induced proteolysis of TOP2 is reduced in the presence of the proteasome inhibitor MG132, indicating that degradation is partly proteasomal. In support of this, siRNA knockdown of 26S proteasome assembly chaperones, such as proteasome maturation protein and p28, also inhibits the etoposide-induced degradation of TOP2B (Ban et al., 2013). The 26S proteasome mediates the degradation of both cytosolic and nuclear protein substrates and consists of a 20S proteolytic core and a 19S regulatory subunit. The 19S subunit facilitates both the unfolding of protein substrates (required to translocate the protein into the narrow proteolytic core) and the regulation of proteasomal degradation by ubiquitination. As such, the 19S subunit also contains ubiquitin binding sites and deubiquitinases, which help target ubiquitinated proteins for degradation (Liu and Jacobson, 2013). While earlier studies involved the study of both unbound TOP2 and DNA-bound TOP2 complexes (usually through western blotting techniques), more recent studies have employed other methods, such as the trapped in agarose DNA immunostaining assay (TARDIS) or in vivo complex of enzymes assay (ICE) to specifically measure levels of drug-stabilized TOP2-DNA covalent complexes in the presence and absence of a proteasome inhibitor. These studies have shown that proteasomal inhibition specifically increases levels of TOP2-DNA complexes following etoposide or teniposide treatment (Alchanati et al., 2009; Fan et al., 2008; Sciascia et al., 2020) and slows the removal of covalently bound TOP2A and TOP2B complexes from DNA (Lee et al., 2016; Swan et al., 2020).

Consistent with the notion that removal of the covalent TOP2 adduct is required to activate a DNA damage response, proteasome inhibition also reduces the etoposide-induced phosphorylation of histone H2AX (Mao et al., 2001; Sciascia et al., 2020; Swan et al., 2020; Zhang et al., 2006). Phosphorylation of histone H2AX is one of the primary events to occur in response to a DSB (Paull et al., 2000), suggesting that proteasome inhibition prevents the conversion of TOP2-DNA covalent complexes to protein-free DSBs. It has also been suggested that proteasome inhibition prevents further processing of the TOP2-DNA covalent complex and instead promotes the spontaneous reversal of TOP2-DNA complexes, which occurs as etoposide dissociates from the DNA after drug washout (Sciascia et al., 2020). Co-treatment of cells with a TOP2 poison and a proteasome inhibitor, such as MG132, also reduces the appearance of other etoposide-induced DNA damage signals, including phosphorylation of ATM, Chk1, and Chk2 (Fan et al., 2008; Zhang et al., 2006).

Interestingly, a number of studies suggest that the proteasomal processing of TOP2A- and TOP2B- DNA covalent complexes is at least partially transcription-dependent (Fan et al., 2008; Mao et al., 2001; Tammaro et al., 2013; Xiao et al., 2003; Zhang et al., 2006). This is unlikely to be due to the expression of a specific gene product, as inhibition of protein synthesis does not impede the TOP2 poison-induced degradation of TOP2B (Mao et al., 2001; Xiao et al., 2003). Instead, it is thought that TOP2 degradation is induced upon collision of the TOP2-DNA covalent complex with elongating RNAPII. Indeed, treatment with etoposide leads to the accumulation of hyperphosphorylated RNAPII, indicative of transcription arrest (Ban et al., 2013). It is conceivable that the processing of TOP2-DNA complexes will also be induced upon collision with DNA polymerases or helicases during replication, and indeed some studies also report a replication-dependent component of TOP2-DNA complex processing (Fan et al., 2008; Tammaro et al., 2013; Yan et al., 2016).

SPRTN-Dependent Degradation of TOP2

SPRTN is another protease which has been implicated in the degradation of TOP2-DNA covalent complexes, as well as other protein-DNA crosslinks induced by camptothecin and formaldehyde (Lopez-Mosqueda et al., 2016; Vaz et al., 2016). While many protein-DNA crosslinks are partly repaired by NER or HR repair, SPRTN was recently identified as the protease required for the processing of crosslinked protein adducts in human cells, in a protease-dependent but proteasome-independent mechanism, which was previously identified in yeast and xenopus (Duxin et al., 2014; Stingele et al., 2014). Unlike the proteasome, SPRTN is a metalloprotease related to the Wss1 protease in yeast (Stingele and Jentsch, 2015), which associates with the replisome to facilitate the clearance of obstructing protein-DNA adducts ahead of the replication fork (Vaz et al., 2016). Consequently, cells lacking SPRTN accumulate both endogenous and exogenous protein-DNA crosslinks and are hypersensitive to protein-DNA crosslink-inducing agents (Stingele et al., 2016; Vaz et al., 2016). Specifically, cells lacking SPRTN display increased sensitivity to etoposide and increased levels of etoposide-induced TOP2-DNA covalent complexes, indicating that drug-stabilized TOP2-DNA covalent complexes are also processed by SPRTN (Lopez-Mosqueda et al., 2016; Vaz et al., 2016). In support of this, SPRTN co-precipitates with TOP2 in vivo (Vaz et al., 2016) and cleaves TOP2 in the presence of ubiquitin in vitro (Lopez-Mosqueda et al., 2016). This could be consistent with a replication-dependent but proteasome-independent mechanism of TOP2-DNA complex processing described by others (Tammaro et al., 2013).

VCP/p97 as Another Factor Involved in Proteolytic Removal of TOP2-DNA Covalent Complexes

VCP/p97 is a protein segregase known to facilitate both proteasome-dependent and SPRTN-dependent degradation of protein substrates. VCP/p97 utilizes its AAA ATPase activity to induce conformational changes in target proteins, which leads to protein unfolding and denaturation. This can aid the extraction of proteins from specific cellular structures which may in turn lead to their degradation by SPRTN or the proteasome.

VCP/p97 and the Proteasome

The 26S proteasome is comprised of a 20S catalytic subunit and a 19S regulatory subunit, the latter of which facilitates the translocation of ubiquitinated protein substrates into the narrow 20S core. Specifically, the 19S subunit contains AAA ATPases and deubiquitinases, which unfold and deubiquitinate target proteins prior to their degradation, respectively. In addition to the 19S hexameric ATPase ring, the proteasome is known to associate with the VCP/p97 AAA ATPase which is also thought to facilitate proteasomal degradation by protein unfolding (Besche et al., 2009; Isakov and Stanhill, 2011). VCP/p97 is increasingly recognized as another important mediator of the ubiquitin-proteasome system, required for the proteasomal degradation of many, but not all, ubiquitinated proteins (Dai and Li, 2001; Heidelberger et al., 2018).

In addition to protein unfolding at the proteasome, VCP/p97 facilitates proteasomal degradation by extracting ubiquitinated proteins from cellular structures, such as the endoplasmic reticulum (ER-associated degradation) or chromatin (chromatin-associated degradation). This is particularly apparent during the DNA damage response, in which VCP/p97 is required for the timely removal of specific DNA repair proteins from chromatin, such as Ku70/80, L3MBTL1, DNA-PKcs and Rad51-Rad52 (Acs et al., 2011; Bergink et al., 2013; Jiang et al., 2013; van den Boom et al., 2016). VCP/p97 is also required for the proteasomal degradation of stalled RNA polymerase II (RNAPII) following UV-induced DNA damage (He et al., 2017; Lafon et al., 2015; Verma et al., 2011).

Notably, previous studies suggest that additional AAA ATPases (such as those associated with RNAPII) are required for the proteasomal degradation of TOP2-DNA covalent complexes after etoposide treatment (Ban et al., 2013), and Wei et al. show that there is an accumulation of ubiquitinated TOP2A in cells depleted of Cdc48 (the yeast homolog of VCP/p97) (Wei et al., 2017). Interestingly, small-molecule inhibition of VCP/p97 slows the removal of both TOP2A- and TOP2B-DNA covalent complexes from chromatin following etoposide treatment, in a manner similar to inhibition of ubiquitination or the proteasome and epistatic to the proteasome pathway (Swan et al., 2021). Consistently, VCP/p97 inhibition also reduces etoposide-induced phosphorylation of histone H2AX, suggesting that VCP/p97 is involved in the processing of TOP2-DNA covalent complexes to protein-free DSBs. Further studies are required to investigate the potential role of VCP/p97 in the proteasomal processing of TOP2-DNA covalent complexes.

VCP/p97 and SPRTN

VCP/p97 is also implicated in the extraction and degradation of proteins by SPRTN, which directly binds and recruits VCP/p97 via a conserved SHP box motif (Mosbech et al., 2012; Stingele et al., 2014). For example, SPRTN recruits VCP/p97 to monoubiquitinated PCNA during replication stress, where it helps to extract TLS polymerase Pol n for degradation and prevents excessive translesion synthesis at DNA damage sites (Davis et al., 2012; Mosbech et al., 2012). Interestingly, SPRTN is also implicated in the proteolysis of both drug-induced and endogenous TOP1-DNA covalent complexes (Maskey et al., 2017; Vaz et al., 2016) in a manner that also involves VCP/p97 (Balakirev et al., 2015; Fielden et al., 2020; Nie et al., 2012; Stingele et al., 2014). Like TOP2, TOP1 can form genotoxic TOP1-DNA covalent complexes following treatment with anticancer drugs, such as camptothecin (CPT), and sensitivity of cells to CPT is significantly increased following depletion of VCP/p97 or SPRTN.

Like the 3′-tyrosyl phosphodiesterase TDP1, which is known to cleave trapped TOP1 covalent complexes from DNA, it is thought that the substrate binding groove of SPRTN is too narrow to facilitate binding of bulky TOP1 adducts (Fielden et al., 2020; Li et al., 2019). It is therefore likely that processing or remodeling of the TOP1 adduct is first required before it can be proteolysed by SPRTN, which may involve the AAA ATPase activity of VCP/p97 (Fielden et al., 2020; Stingele et al., 2014). Fielden et al. identify a specialized complex containing VCP/p97 and the VCP/p97 cofactor TEX264 which is required for the clearance of TOP1-DNA covalent complexes (but not other protein-DNA adducts) by SPRTN (Fielden et al., 2020). While it is possible that VCP/p97 may also facilitate the SPRTN-dependent proteolysis of TOP2-DNA covalent complexes, this remains to be investigated.

Tyrosyl DNA Phosphodiesterase 2 (TDP2)

TDP2 is a 5′-phosphodiesterase which directly cleaves the covalent linkage between trapped TOP2 complexes and DNA (Cortes Ledesma et al., 2009; Zeng et al., 2011), producing a clean and ligatable DSB for NHEJ repair (Gómez-Herreros et al., 2013; Schellenberg et al., 2016). TDP2 has been shown to be particularly important for the repair of TOP2-mediated DSBs during transcription and in response to androgens, which can otherwise lead to oncogenic chromosome translocations and genome instability (Al Mahmud et al., 2020; Gómez-Herreros et al., 2017). However, due to inaccessibility of the 5′-phosphotyrosyl linkage within the TOP2 active site, TDP2 alone cannot remove the TOP2-DNA complex until it has been proteolysed or denatured (Gao et al., 2014; Lee et al., 2018; Schellenberg et al., 2012). For example, proteasomal degradation of the TOP2 adduct leaves behind a small 5′-phosphotyrosyl peptide, which can then be directly removed by TDP2 prior to NHEJ repair (Gao et al., 2014). It is conceivable that proteolysis via the proteasome or SPRTN could facilitate TDP2-dependent repair of the remaining 5′-phosphotyrosyl adduct. Alternatively, the ZATT SUMO ligase was identified as an additional factor which can facilitate the direct removal of TOP2-DNA covalent complexes by TDP2 in a proteasome-independent manner (Schellenberg et al., 2017; Zagnoli-Vieira and Caldecott, 2017). Specifically, it is proposed that SUMOylation of the TOP2-DNA covalent complex and binding of ZATT leads to a conformational change in TOP2, negating the need for TOP2 proteolysis and enabling direct access of TDP2 to the phosphodiester bond for cleavage.

Nucleolytic Mechanisms

Alternatively, ligatable ends can be produced by nucleolytic processing of the TOP2-DNA complex, whereby the TOP2 protein is removed through cleavage of the adjacent DNA by a nuclease like Mre11. The ssDNA endonuclease activity of Mre11 is implicated in the resection of blocked DNA ends, which induces a nick in the 5′ strand close to the 5′ adduct (Garcia et al., 2011). This allows the 3′-5′ exonuclease digestion of DNA by Mre11, consistent with its 3′-5′ exonuclease activity in vitro and creates a gap for loading of the 5′-3′ exonucleases Exo1 and DNA2 (Wang et al., 2017). The endonuclease and exonuclease activity of Mre11 is known to be stimulated by another endonuclease, CtIP, in yeast and human cells (Anand et al., 2016; Cannavo and Cejka, 2014; Deshpande et al., 2016; Sartori et al., 2007). Interestingly, DNA2 may be required for the initiation of resection at clean DSBs, suggesting distinct initiation pathways for clean versus blocked DNA ends (Paudyal et al., 2017).

Evidence suggesting that Mre11 can remove covalently linked 5′ adducts from DNA ends comes from study of the TOP2-like protein, Spo11. Spo11 induces enzyme-linked DSBs during meiosis to facilitate meiotic recombination. In contrast to TOP2, the Spo11-induced DSB is not religated, and the covalently linked 5′-Spo11 adduct must be removed to allow recombination between broken strands (Neale et al., 2005). Mre11/CtIP is required for the removal of Spo11 adducts from DNA ends in S. cerevisiae (Moreau et al., 1999; Neale et al., 2005), and the removal of Rec12 (the Spo11 homolog) in S. pombe (Milman et al., 2009; Rothenberg et al., 2009). Various studies have since shown that 5′-TOP2 DNA adducts are also removed in an Mre11/CtIP-dependent manner in yeast and human cells. S. Pombe Mre11 mutants deficient in Spo11 removal are hypersensitive to TOP-53 (an etoposide derivative) and display increased levels of drug-induced TOP2-DNA complexes compared with wild-type cells (Hartsuiker et al., 2009). A number of studies have also shown an increase in levels of drug-induced TOP2-DNA complexes and drug sensitivity in Mre11- or CtIP-depleted cells, including chicken and human cells (Aparicio et al., 2016; Hamilton and Maizels, 2010; Hoa et al., 2016; Lee et al., 2012; Nakamura et al., 2010; Takeda et al., 2016). Once the TOP2 adduct is removed, etoposide-induced DSBs can then be resected by DNA2 (Tammaro et al., 2016). Interestingly, depletion or inhibition of Mre11 leads to the accumulation of TOP2-DNA complexes even in the absence of a TOP2 poison, indicating that Mre11 is important for the removal of endogenously trapped TOP2-DNA complexes (Hoa et al., 2016; Lee et al., 2012). This suggests that Mre11 preserves genome stability, not only through HR repair but also by processing abortive TOP2 reactions.

Studies in mammalian cells have shown that breast cancer type 1 susceptibility protein (BRCA1) is required for the removal of both endogenous and drug-induced 5′-TOP2 adducts by Mre11/CtIP (Aparicio et al., 2016; Morimoto et al., 2019; Nakamura et al., 2010; Sasanuma et al., 2018). Aparicio et al. show that the association of BRCA1 with chromatin was significantly greater after etoposide treatment than after the induction of endonuclease-induced “clean” DSBs, and depletion of BRCA1 increases levels of etoposide-induced TOP2-DNA complexes similarly to CtIP or Mre11 depletion (Aparicio et al., 2016). Specifically, the interaction between BRCA1 and CtIP was required for the CtIP-dependent removal of etoposide-induced TOP2-DNA complexes. Notably, BRCA1 is also implicated in the degradation of TOP1 and TOP2B following CPT or etoposide treatment, respectively (Sordet et al., 2008; Xiao and Goodrich, 2005).

Despite its well-known role in HR, Mre11-mediated processing of TOP2-DNA complexes can lead to NHEJ repair of the TOP2-induced DSB in G0/G1 arrested cells, where resection does not occur due to absence of a homologous sister chromatid prior to DNA replication (Akagawa et al., 2020; Quennet et al., 2011). Sasunama et al. also demonstrate that BRCA1 is required for the NHEJ repair of DSBs with TOP2 adducts, but not for the NHEJ repair of clean breaks (Sasanuma et al., 2018). The Mre11-dependent repair of etoposide-induced DSBs is epistatic with Ku but not TDP2, indicating that Mre11-dependent repair facilitates NHEJ in a manner distinct from the TDP2 pathway (Hoa et al., 2016). This suggests that NHEJ repair of etoposide-induced DSBs can occur via at least two different mechanisms: one involving the TDP2-dependent removal of proteolysed or denatured TOP2 adducts (Gómez-Herreros et al., 2013), and one involving the Mre11/CtIP/BRCA1-dependent removal of TOP2 adducts (Liao et al., 2016). It is unclear whether in vivo nucleolytic removal by the Mre11/CtIP/BRCA1 pathway can occur without prior processing of the TOP2 protein adduct, or if nucleolytic processing could provide an alternative “end polishing” step after TOP2 proteolysis or denaturation, similar to TDP2. However, MRE11 is able to remove TOP2A from TOP2A-DNA complexes in vitro in the absence of prior proteolytic processing (Lee et al., 2012).

Role of Post-Translational Modifications

In addition to the diversity of pathways described above, the complexity of TOP2-DNA covalent complex repair is further expanded by additional layers of regulation achieved through post-translational modifications like ubiquitination and SUMOylation. Both are implicated in a number of these pathways, as summarized below.

Ubiquitination in TOP2-DNA Covalent Complex Repair

Ubiquitin is a small 76-amino acid peptide which is conjugated to the lysine residues of target proteins in a 3 step cascade involving an E1-activating enzyme, an E2 conjugating enzyme and an E3 ligating enzyme. This culminates in an isopeptide bond between ubiquitin and the target lysine residue, which is readily reversed by enzymes known as deubiquitinases. The conjugation of ubiquitin to target proteins (i.e., ubiquitination) regulates many cellular processes, such as protein-protein interactions and proteasomal degradation. Ubiquitin itself contains 7 lysine residues (K6, K11, K27, K29, K33, K48, and K63), which can also be ubiquitinated, forming polyubiquitin chains with different topologies and functions (Komander, 2009). Typically, the conjugation of K11- or K48-linked ubiquitin chains to a target protein is associated with the degradation of the ubiquitinated protein by the proteasome, while K63-linked chains are frequently involved in protein–protein interactions (Akutsu et al., 2016; Komander and Rape, 2012). Thus, ubiquitination helps to regulate the timely recruitment and removal of repair proteins in various signaling pathways, including the DNA damage response (Jackson and Durocher, 2013).

In addition to the well-established role of ubiquitin in the regulation of proteasomal degradation, ubiquitination is also involved in the regulation of SPRTN-dependent proteolysis. A large proportion of SPRTN is constitutively monoubiquitinated but is rapidly deubiquitinated upon the induction of genotoxic protein-DNA adducts by formaldehyde (Stingele et al., 2016; Zhao et al., 2021). Two deubiquitinase enzymes, namely VCPIP1/VCIP135 and Usp7, were recently shown to activate SPRTN-dependent proteolysis. While VCPIP1/VCIP135 is proposed to regulate binding of SPRTN to DNA (Huang et al., 2020; Stingele et al., 2016), Usp7 increases SPRTN activity by preventing its inactivation by autocatalytic cleavage (Zhao et al., 2021). SPRTN also contains a UBZ domain, which facilitates the recruitment of SPRTN to ubiquitinated protein-DNA adducts (Davis et al., 2012; Mosbech et al., 2012). Therefore, ubiquitination regulates protein degradation not only by signaling proteins for their destruction by the proteasome, but also by regulating SPRTN-dependent proteolysis.

TOP2 Ubiquitination

As TOP2-DNA covalent complexes are processed both via proteasome- and SPRTN- dependent mechanisms, a number of studies have also investigated the role of ubiquitination in TOP2-DNA covalent complex repair. While some discrepancies exist in the literature (Ban et al., 2013; Mao et al., 2001), recent studies have shown that the removal of TOP2A- and TOP2B- DNA adducts, and the subsequent appearance of TOP2 poison-induced DSBs, is partly ubiquitin-dependent and epistatic with the proteasomal pathway (Swan et al., 2020). This seems to be mediated in part by the polyubiquitination of TOP2 by the E3 ubiquitin ligase BMI1/RING1A and the SUMO-targeted ubiquitin ligase RNF4, which leads to proteasomal degradation (Alchanati et al., 2009; Sun et al., 2019; Swan et al., 2020). Indeed, a number of E3 ubiquitin ligases have been shown to ubiquitinate TOP2 in various stress conditions, such as glucose starvation, HDAC inhibition and ICRF-193 treatment, which leads to the proteasomal degradation of TOP2 (Chen et al., 2011; Isik et al., 2003; Yun et al., 2009). Indeed, constitutive monoubiquitination by the Smurf2 ubiquitin ligase may protect TOP2 from polyubiquitin-dependent proteasomal degradation (Emanuelli et al., 2017). The ubiquitin-proteasome system is also known to regulate levels of TOP2A during the cell cycle (Eguren et al., 2014; Salmena et al., 2001).

While numerous ubiquitination sites have been identified on TOP2A and TOP2B (Kim et al., 2011), other studies have been unable to detect the polyubiquitination of TOP2A or TOP2B in the presence or absence of etoposide (Ban et al., 2013). Instead, Ban et al. propose a mechanism of TOP2B-DNA complex processing by the proteasome, which does not require prior ubiquitination of TOP2. In this model, TOP2B is proteasomally degraded following collision of the TOP2B-DNA complex with elongating RNAPII, in which the proteasome is recruited to TOP2B via RNAPII-associated 19S AAA ATPases rather than a polyubiquitin signal (Ban et al., 2013).

Ubiquitination may also be required for the modification of other proteins involved in TOP2-DNA complex repair. For example, TDP2 contains an N-terminal ubiquitin binding domain (UBA-like domain) which is required for TDP2 activity and the TDP2-dependent repair of etoposide-induced TOP2-DNA covalent complexes (Rao et al., 2016). While it was later shown that the TDP2 UBA domain binds K27- and K63-linked polyubiquitin chains, the TDP2 UBA does not bind ubiquitinated TOP2 and the ubiquitinated target required for TDP2-dependent repair remains unknown. It is speculated that a ubiquitin-dependent interaction involving the TDP2 UBA domain may induce a conformational change in the TDP2 active site or may mediate interactions with ubiquitinated histones at DNA damage sites (Kawale and Povirk, 2018; Rao et al., 2016; Schellenberg et al., 2020).

Ubiquitination, and specifically the E2 ubiquitin-conjugating enzyme Ubc13, has also been recently implicated in the Mre11-dependent processing pathway. Expression of a catalytically inactive Ubc13 mutant leads to the accumulation of etoposide-induced DSBs in a manner that was epistatic to the Mre11 pathway but independent of the TDP2 pathway (Akagawa et al., 2020). Ubc13 is known to mediate K63-linked ubiquitination at DNA damage sites, leading to the recruitment of various repair proteins including BRCA1 and Rap80. Akagowa et al. showed that Ubc13 also mediates the recruitment of Mre11 to etoposide-induced TOP2 adducts (as well as other IR-induced “dirty” DNA ends). While the ubiquitinated target also remains unknown, Ubc13 (together with Rap80) was required for the etoposide-induced interaction between Mre11 and BRCA1 and subsequent activation of the Mre11 nuclease.

It is well known that other post-translational modifications, such as SUMOylation or phosphorylation, can promote protein ubiquitination, such as inducing changes in chromatin conformation or the recruitment of ubiquitin ligases. For example, phosphorylation of TOP2A in response to HDAC inhibition (first by casein kinase II and then by glycogen synthase kinase 3β), leads to the recruitment of the ubiquitin ligase Fbw7 and ubiquitin-dependent proteasomal degradation (Chen et al., 2011). Phosphorylation of TOP2A is also required for its ubiquitination by BRCA1, which does not lead to proteasomal degradation but to increased decatenation activity (Lou et al., 2005). This emphasizes the importance of other post-translational modifications in the regulation of TOP2 ubiquitination. Indeed, SUMOylation of TOP2 can directly lead to the RNF4/ubiquitin-dependent proteasomal degradation of etoposide-induced TOP2-DNA complexes, as discussed further below (Sun et al., 2019).

SUMOylation in TOP2-DNA Covalent Complex Repair

Like ubiquitin, SUMO is conjugated to the lysine residues of target proteins in a 3-step catalytic cascade involving an E1, E2, and E3 enzyme. There are three isoforms of SUMO in human cells (four including the tissue specific SUMO-4); SUMO-1, SUMO-2 and SUMO-3 (Geiss-Friedlander and Melchior, 2007). However, SUMO-2 and SUMO-3 share 97% homology and are often referred to as SUMO-2/3. Like ubiquitin, SUMO can form polySUMO chains, although this is restricted to SUMO-2/3 and not SUMO-1. Interestingly, polySUMO chains are recognized by a class of E3 ubiquitin ligases known as SUMO-targeted ubiquitin ligases. SUMO-targeted ubiquitin ligases (RNF4 or RNF111 in mammalian cells) contain a SUMO-interacting motif (SIM) which binds non-covalently to SUMO and recruits the ubiquitin ligase to SUMOylated proteins (Geoffroy and Hay, 2009). In this way, SUMOylation of proteins, including SUMOylated TOP2-DNA covalent complexes, can lead to their ubiquitin-dependent degradation by the proteasome.

A number of studies have shown that TOP2 is SUMOylated in response to etoposide, teniposide, and the TOP2 inhibitor ICRF-193 (Agostinho et al., 2008; Isik et al., 2003; Lee et al., 2018; Mao et al., 2000; Schellenberg et al., 2017; Sun et al., 2019), and this seems to occur prior to TOP2 ubiquitination (Isik et al., 2003; Sun et al., 2019). It is now known that SUMOylation of TOP2 by the ZATT E3 ligase facilitates the direct removal of TOP2-DNA covalent complexes by TDP2, as discussed above (Schellenberg et al., 2017). However, SUMO is also implicated in other relevant pathways, which may affect TOP2-DNA covalent complex processing and repair, such as the SPRTN-dependent pathway (Vaz et al., 2020) and the recently described RNF4/ubiquitin-dependent proteasomal pathway. Following the SUMOylation of TOP2 by the E3 SUMO ligase PIAS4, RNF4 is recruited to TOP2 via its SIM domain and polyubiquitinates TOP2 with K48-linked chains, thereby leading to degradation of TOP2 by the proteasome (Sun et al., 2019). Indeed, knockdown or knockout of RNF4 increases levels of TOP2-DNA complexes and reduces the appearance of etoposide-induced γH2AX levels in a manner which is epistatic with the proteasomal processing pathway (Sciascia et al., 2020; Sun et al., 2019). However, levels of etoposide-induced histone H2AX phosphorylation were higher in RNF4−/− MEFs compared with proteasome-inhibited cells, consistent with the involvement of other ubiquitin ligases in the ubiquitin/proteasome-dependent pathway (Sciascia et al., 2020), such as BMI1/RING1A. Therefore, in addition to the ZATT/TDP2 pathway, SUMOylation of TOP2 can also lead to the proteasomal degradation of TOP2 adducts through RNF4-dependent polyubiquitination of TOP2.

Conclusion

Numerous pathways have now been described which facilitate the removal and repair of TOP2-DNA covalent complexes (Fig. 1). The existence of multiple redundant pathways is now known. These ensure the timely and efficient repair of TOP2-DNA covalent adducts, thereby maintaining genome stability. However, it is not known what determines which repair pathway is used. Recent work also emphasizes the important role of post-translational modifications, such as ubiquitination and , in the regulation of TOP2-DNA complex repair. There remain many unanswered questions about the timing and function of these post-translational modifications. Whether modulation of the removal and repair of TOP2-DNA covalent complexes with small molecule inhibitors will help to improve outcomes of TOP2 poison chemotherapy, for example by increasing TOP2 poison cytotoxicity and reducing genotoxicity, remains to be determined in preclinical model systems.

Fig. 1.
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Fig. 1.

Schematic showing the routes to process TOP2-DNA covalent complexes to protein-free double-strand breaks suitable for DNA repair.

Authorship Contributions

Wrote or contributed to the writing of the manuscript: Swan, Cowell, Austin.

Footnotes

    • Received July 21, 2021.
    • Accepted October 11, 2021.
  • This study was supported by Bloodwise Research Specialist Program Grant [12031] and by a Bloodwise Gordon Piller Studentship [13063].

  • The authors declare that they have no conflict of interest.

  • https://doi.org/10.1124/molpharm.121.000374.

Abbreviations

BRCA1
breast cancer type 1 susceptibility protein
CPT
camptothecin
DSB
double-strand break
HR
homologous recombination
NHEJ
non-homologous end joining
TDP2
tyrosyl DNA phosphodiesterase 2
TOP1
topoisomerase I
TOP2
topoisomerase II
  • Copyright © 2021 by The American Society for Pharmacology and Experimental Therapeutics

References

  1. ↵
    1. Acs K,
    2. Luijsterburg MS,
    3. Ackermann L,
    4. Salomons FA,
    5. Hoppe T, and
    6. Dantuma NP
    (2011) The AAA-ATPase VCP/p97 promotes 53BP1 recruitment by removing L3MBTL1 from DNA double-strand breaks. Nat Struct Mol Biol 18:1345–1350 DOI: 10.1038/nsmb.2188.
    OpenUrlCrossRefPubMed
  2. ↵
    1. Adachi N,
    2. Suzuki H,
    3. Iiizumi S, and
    4. Koyama H
    (2003) Hypersensitivity of nonhomologous DNA end-joining mutants to VP-16 and ICRF-193: implications for the repair of topoisomerase II-mediated DNA damage. J Biol Chem 278:35897–35902 DOI: 10.1074/jbc.M306500200.
    OpenUrlAbstract/FREE Full Text
  3. ↵
    1. Agostinho M,
    2. Santos V,
    3. Ferreira F,
    4. Costa R,
    5. Cardoso J,
    6. Pinheiro I,
    7. Rino J,
    8. Jaffray E,
    9. Hay RT, and
    10. Ferreira J
    (2008) Conjugation of human topoisomerase 2 α with small ubiquitin-like modifiers 2/3 in response to topoisomerase inhibitors: cell cycle stage and chromosome domain specificity. Cancer Res 68:2409–2418 DOI: 10.1158/0008-5472.CAN-07-2092.
    OpenUrlAbstract/FREE Full Text
  4. ↵
    1. Akagawa R,
    2. Trinh HT,
    3. Saha LK,
    4. Tsuda M,
    5. Hirota K,
    6. Yamada S,
    7. Shibata A,
    8. Kanemaki MT,
    9. Nakada S,
    10. Takeda S, et al.
    (2020) UBC13-Mediated Ubiquitin Signaling Promotes Removal of Blocking Adducts from DNA Double-Strand Breaks. iScience 23:101027–101027 DOI: 10.1016/j.isci.2020.101027.
    OpenUrl
  5. ↵
    1. Akutsu M,
    2. Dikic I, and
    3. Bremm A
    (2016) Ubiquitin chain diversity at a glance. J Cell Sci 129:875–880 DOI: 10.1242/jcs.183954.
    OpenUrlAbstract/FREE Full Text
  6. ↵
    1. Al Mahmud MR,
    2. Ishii K,
    3. Bernal-Lozano C,
    4. Delgado-Sainz I,
    5. Toi M,
    6. Akamatsu S,
    7. Fukumoto M,
    8. Watanabe M,
    9. Takeda S,
    10. Cortés-Ledesma F, et al.
    (2020) TDP2 suppresses genomic instability induced by androgens in the epithelial cells of prostate glands. Genes Cells 25:450–465 DOI: 10.1111/gtc.12770.
    OpenUrl
  7. ↵
    1. Alchanati I,
    2. Teicher C,
    3. Cohen G,
    4. Shemesh V,
    5. Barr HM,
    6. Nakache P,
    7. Ben-Avraham D,
    8. Idelevich A,
    9. Angel I,
    10. Livnah N, et al.
    (2009) The E3 ubiquitin-ligase Bmi1/Ring1A controls the proteasomal degradation of Top2α cleavage complex – a potentially new drug target. PLoS One 4:e8104 DOI: 10.1371/journal.pone.0008104.
    OpenUrlCrossRefPubMed
  8. ↵
    1. Anand R,
    2. Ranjha L,
    3. Cannavo E, and
    4. Cejka P
    (2016) Phosphorylated CtIP Functions as a Co-factor of the MRE11-RAD50-NBS1 Endonuclease in DNA End Resection. Mol Cell 64:940–950 DOI: 10.1016/j.molcel.2016.10.017.
    OpenUrlCrossRefPubMed
  9. ↵
    1. Aparicio T,
    2. Baer R,
    3. Gottesman M, and
    4. Gautier J
    (2016) MRN, CtIP, and BRCA1 mediate repair of topoisomerase II-DNA adducts. J Cell Biol 212:399–408 DOI: 10.1083/jcb.201504005.
    OpenUrlAbstract/FREE Full Text
  10. ↵
    1. Balakirev MY,
    2. Mullally JE,
    3. Favier A,
    4. Assard N,
    5. Sulpice E,
    6. Lindsey DF,
    7. Rulina AV,
    8. Gidrol X, and
    9. Wilkinson KD
    (2015) Wss1 metalloprotease partners with Cdc48/Doa1 in processing genotoxic SUMO conjugates. eLife 4:e06763 DOI: 10.7554/eLife.06763.
    OpenUrlCrossRef
  11. ↵
    1. Ban Y,
    2. Ho C-W,
    3. Lin R-K,
    4. Lyu YL, and
    5. Liu LF
    (2013) Activation of a novel ubiquitin-independent proteasome pathway when RNA polymerase II encounters a protein roadblock. Mol Cell Biol 33:4008–4016 DOI: 10.1128/mcb.00403-13.
    OpenUrlAbstract/FREE Full Text
  12. ↵
    1. Bergink S,
    2. Ammon T,
    3. Kern M,
    4. Schermelleh L,
    5. Leonhardt H, and
    6. Jentsch S
    (2013) Role of Cdc48/p97 as a SUMO-targeted segregase curbing Rad51-Rad52 interaction. Nat Cell Biol 15:526–532 DOI: 10.1038/ncb2729.
    OpenUrlCrossRefPubMed
  13. ↵
    1. Besche HC,
    2. Haas W,
    3. Gygi SP, and
    4. Goldberg AL
    (2009) Isolation of mammalian 26S proteasomes and p97/VCP complexes using the ubiquitin-like domain from HHR23B reveals novel proteasome-associated proteins. Biochemistry 48:2538–2549 DOI: 10.1021/bi802198q.
    OpenUrlCrossRefPubMed
  14. ↵
    1. Cannavo E and
    2. Cejka P
    (2014) Sae2 promotes dsDNA endonuclease activity within Mre11-Rad50-Xrs2 to resect DNA breaks. Nature 514:122–125 DOI: 10.1038/nature13771.
    OpenUrlCrossRefPubMed
  15. ↵
    1. Chen M-C,
    2. Chen C-H,
    3. Chuang H-C,
    4. Kulp SK,
    5. Teng C-M, and
    6. Chen C-S
    (2011) Novel mechanism by which histone deacetylase inhibitors facilitate topoisomerase IIα degradation in hepatocellular carcinoma cells. Hepatology 53:148–159 DOI: 10.1002/hep.23964.
    OpenUrlCrossRefPubMed
  16. ↵
    1. Cowell IG,
    2. Sondka Z,
    3. Smith K,
    4. Lee KC,
    5. Manville CM,
    6. Sidorczuk-Lesthuruge M,
    7. Rance HA,
    8. Padget K,
    9. Jackson GH,
    10. Adachi N, et al.
    (2012) Model for MLL translocations in therapy-related leukemia involving topoisomerase IIβ-mediated DNA strand breaks and gene proximity. Proc Natl Acad Sci USA 109:8989–8994 DOI: 10.1073/pnas.1204406109.
    OpenUrlAbstract/FREE Full Text
  17. ↵
    1. Dai RM and
    2. Li CC
    (2001) Valosin-containing protein is a multi-ubiquitin chain-targeting factor required in ubiquitin-proteasome degradation. Nat Cell Biol 3:740–744 DOI: 10.1038/35087056.
    OpenUrlCrossRefPubMed
  18. ↵
    1. D’Arpa P,
    2. Beardmore C, and
    3. Liu LF
    (1990) Involvement of nucleic acid synthesis in cell killing mechanisms of topoisomerase poisons. Cancer Res 50:6919–6924.
    OpenUrlAbstract/FREE Full Text
  19. ↵
    1. Davis EJ,
    2. Lachaud C,
    3. Appleton P,
    4. Macartney TJ,
    5. Näthke I, and
    6. Rouse J
    (2012) DVC1 (C1orf124) recruits the p97 protein segregase to sites of DNA damage. Nat Struct Mol Biol 19:1093–1100 DOI: 10.1038/nsmb.2394.
    OpenUrlCrossRefPubMed
  20. ↵
    1. Deshpande RA,
    2. Lee J-H,
    3. Arora S, and
    4. Paull TT
    (2016) Nbs1 Converts the Human Mre11/Rad50 Nuclease Complex into an Endo/Exonuclease Machine Specific for Protein-DNA Adducts. Mol Cell 64:593–606 DOI: 10.1016/j.molcel.2016.10.010.
    OpenUrlCrossRef
  21. ↵
    1. Deweese JE and
    2. Osheroff N
    (2009) The DNA cleavage reaction of topoisomerase II: wolf in sheep’s clothing. Nucleic Acids Res 37:738–748 DOI: 10.1093/nar/gkn937.
    OpenUrlCrossRefPubMed
  22. ↵
    1. Duxin JP,
    2. Dewar JM,
    3. Yardimci H, and
    4. Walter JC
    (2014) Repair of a DNA-protein crosslink by replication-coupled proteolysis. Cell 159:346–357 DOI: 10.1016/j.cell.2014.09.024.
    OpenUrlCrossRefPubMed
  23. ↵
    1. Eguren M,
    2. Álvarez-Fernández M,
    3. García F,
    4. López-Contreras AJ,
    5. Fujimitsu K,
    6. Yaguchi H,
    7. Luque-García JL,
    8. Fernández-Capetillo O,
    9. Muñoz J,
    10. Yamano H, et al.
    (2014) A synthetic lethal interaction between APC/C and topoisomerase poisons uncovered by proteomic screens. Cell Rep 6:670–683 DOI: 10.1016/j.celrep.2014.01.017.
    OpenUrlCrossRefPubMed
  24. ↵
    1. Emanuelli A,
    2. Borroni AP,
    3. Apel-Sarid L,
    4. Shah PA,
    5. Ayyathan DM,
    6. Koganti P,
    7. Levy-Cohen G, and
    8. Blank M
    (2017) Smurf2-Mediated Stabilization of DNA Topoisomerase IIα Controls Genomic Integrity. Cancer Res 77:4217–4227 DOI: 10.1158/0008-5472.CAN-16-2828.
    OpenUrlAbstract/FREE Full Text
  25. ↵
    1. Fan J-R,
    2. Peng A-L,
    3. Chen H-C,
    4. Lo S-C,
    5. Huang T-H, and
    6. Li T-K
    (2008) Cellular processing pathways contribute to the activation of etoposide-induced DNA damage responses. DNA Repair (Amst) 7:452–463 DOI: 10.1016/j.dnarep.2007.12.002.
    OpenUrlCrossRefPubMed
  26. ↵
    1. Fielden J,
    2. Wiseman K,
    3. Torrecilla I,
    4. Li S,
    5. Hume S,
    6. Chiang S-C,
    7. Ruggiano A,
    8. Narayan Singh A,
    9. Freire R,
    10. Hassanieh S, et al.
    (2020) TEX264 coordinates p97- and SPRTN-mediated resolution of topoisomerase 1-DNA adducts. Nat Commun 11:1274 DOI: 10.1038/s41467-020-15000-w.
    OpenUrl
  27. ↵
    1. Gao R,
    2. Schellenberg MJ,
    3. Huang S-YN,
    4. Abdelmalak M,
    5. Marchand C,
    6. Nitiss KC,
    7. Nitiss JL,
    8. Williams RS, and
    9. Pommier Y
    (2014) Proteolytic degradation of topoisomerase II (Top2) enables the processing of Top2·DNA and Top2·RNA covalent complexes by tyrosyl-DNA-phosphodiesterase 2 (TDP2). J Biol Chem 289:17960–17969 DOI: 10.1074/jbc.M114.565374.
    OpenUrlAbstract/FREE Full Text
  28. ↵
    1. Garcia V,
    2. Phelps SEL,
    3. Gray S, and
    4. Neale MJ
    (2011) Bidirectional resection of DNA double-strand breaks by Mre11 and Exo1. Nature 479:241–244 DOI: 10.1038/nature10515.
    OpenUrlCrossRefPubMed
  29. ↵
    1. Geiss-Friedlander R and
    2. Melchior F
    (2007) Concepts in sumoylation: a decade on. Nat Rev Mol Cell Biol 8:947–956 DOI: 10.1038/nrm2293.
    OpenUrlCrossRefPubMed
  30. ↵
    1. Geoffroy M-C and
    2. Hay RT
    (2009) An additional role for SUMO in ubiquitin-mediated proteolysis. Nat Rev Mol Cell Biol 10:564–568 DOI: 10.1038/nrm2707.
    OpenUrlCrossRefPubMed
  31. ↵
    1. Gómez-Herreros F,
    2. Romero-Granados R,
    3. Zeng Z,
    4. Alvarez-Quilón A,
    5. Quintero C,
    6. Ju L,
    7. Umans L,
    8. Vermeire L,
    9. Huylebroeck D,
    10. Caldecott KW, et al.
    (2013) TDP2-dependent non-homologous end-joining protects against topoisomerase II-induced DNA breaks and genome instability in cells and in vivo. PLoS Genet 9:e1003226 DOI: 10.1371/journal.pgen.1003226.
    OpenUrlCrossRefPubMed
  32. ↵
    1. Gómez-Herreros F,
    2. Zagnoli-Vieira G,
    3. Ntai I,
    4. Martínez-Macías MI,
    5. Anderson RM,
    6. Herrero-Ruíz A, and
    7. Caldecott KW
    (2017) TDP2 suppresses chromosomal translocations induced by DNA topoisomerase II during gene transcription. Nat Commun 8:233 DOI: 10.1038/s41467-017-00307-y.
    OpenUrlCrossRef
  33. ↵
    1. Hamilton NK and
    2. Maizels N
    (2010) MRE11 function in response to topoisomerase poisons is independent of its function in double-strand break repair in Saccharomyces cerevisiae. PLoS One 5:e15387 DOI: 10.1371/journal.pone.0015387.
    OpenUrlCrossRefPubMed
  34. ↵
    1. Hartsuiker E,
    2. Neale MJ, and
    3. Carr AM
    (2009) Distinct requirements for the Rad32(Mre11) nuclease and Ctp1(CtIP) in the removal of covalently bound topoisomerase I and II from DNA. Mol Cell 33:117–123 DOI: 10.1016/j.molcel.2008.11.021.
    OpenUrlCrossRefPubMed
  35. ↵
    1. He J,
    2. Zhu Q,
    3. Wani G, and
    4. Wani AA
    (2017) UV-induced proteolysis of RNA polymerase II is mediated by VCP/p97 segregase and timely orchestration by Cockayne syndrome B protein. Oncotarget 8:11004–11019 DOI: 10.18632/oncotarget.14205.
    OpenUrlCrossRef
  36. ↵
    1. Heidelberger JB,
    2. Voigt A,
    3. Borisova ME,
    4. Petrosino G,
    5. Ruf S,
    6. Wagner SA, and
    7. Beli P
    (2018) Proteomic profiling of VCP substrates links VCP to K6-linked ubiquitylation and c-Myc function. EMBO Rep 19:e44754 DOI: 10.15252/embr.201744754.
    OpenUrlAbstract/FREE Full Text
  37. ↵
    1. Hoa NN,
    2. Shimizu T,
    3. Zhou ZW,
    4. Wang Z-Q,
    5. Deshpande RA,
    6. Paull TT,
    7. Akter S,
    8. Tsuda M,
    9. Furuta R,
    10. Tsutsui K, et al.
    (2016) Mre11 Is Essential for the Removal of Lethal Topoisomerase 2 Covalent Cleavage Complexes. Mol Cell 64:580–592 DOI: 10.1016/j.molcel.2016.10.011.
    OpenUrlCrossRef
  38. ↵
    1. Huang J,
    2. Zhou Q,
    3. Gao M,
    4. Nowsheen S,
    5. Zhao F,
    6. Kim W,
    7. Zhu Q,
    8. Kojima Y,
    9. Yin P,
    10. Zhang Y, et al.
    (2020) Tandem Deubiquitination and Acetylation of SPRTN Promotes DNA-Protein Crosslink Repair and Protects against Aging. Mol Cell 79:824–835.e5 DOI: 10.1016/j.molcel.2020.06.027.
    OpenUrlCrossRef
  39. ↵
    1. Isakov E and
    2. Stanhill A
    (2011) Stalled proteasomes are directly relieved by P97 recruitment. J Biol Chem 286:30274–30283 DOI: 10.1074/jbc.M111.240309.
    OpenUrlAbstract/FREE Full Text
  40. ↵
    1. Isik S,
    2. Sano K,
    3. Tsutsui K,
    4. Seki M,
    5. Enomoto T,
    6. Saitoh H, and
    7. Tsutsui K
    (2003) The SUMO pathway is required for selective degradation of DNA topoisomerase IIbeta induced by a catalytic inhibitor ICRF-193(1). FEBS Lett 546:374–378 DOI: 10.1016/S0014-5793(03)00637-9.
    OpenUrlCrossRefPubMed
  41. ↵
    1. Jackson SP and
    2. Durocher D
    (2013) Regulation of DNA damage responses by ubiquitin and SUMO. Mol Cell 49:795–807 DOI: 10.1016/j.molcel.2013.01.017.
    OpenUrlCrossRefPubMed
  42. ↵
    1. Jiang N,
    2. Shen Y,
    3. Fei X,
    4. Sheng K,
    5. Sun P,
    6. Qiu Y,
    7. Larner J,
    8. Cao L,
    9. Kong X, and
    10. Mi J
    (2013) Valosin-containing protein regulates the proteasome-mediated degradation of DNA-PKcs in glioma cells. Cell Death Dis 4:e647 DOI: 10.1038/cddis.2013.171.
    OpenUrlCrossRefPubMed
  43. ↵
    1. Kawale AS and
    2. Povirk LF
    (2018) Tyrosyl-DNA phosphodiesterases: rescuing the genome from the risks of relaxation. Nucleic Acids Res 46:520–537 DOI: 10.1093/nar/gkx1219.
    OpenUrlCrossRef
  44. ↵
    1. Kim W,
    2. Bennett EJ,
    3. Huttlin EL,
    4. Guo A,
    5. Li J,
    6. Possemato A,
    7. Sowa ME,
    8. Rad R,
    9. Rush J,
    10. Comb MJ et al.
    (2011) Systematic and quantitative assessment of the ubiquitin-modified proteome. Mol Cell 44:325–340 DOI: 10.1016/j.molcel.2011.08.025.
    OpenUrlCrossRefPubMed
  45. ↵
    1. Komander D
    (2009) The emerging complexity of protein ubiquitination. Biochem Soc Trans 37:937–953 DOI: 10.1042/BST0370937.
    OpenUrlAbstract/FREE Full Text
  46. ↵
    1. Komander D and
    2. Rape M
    (2012) The ubiquitin code. Annu Rev Biochem 81:203–229 DOI: 10.1146/annurev-biochem-060310-170328.
    OpenUrlCrossRefPubMed
  47. ↵
    1. Lafon A,
    2. Taranum S,
    3. Pietrocola F,
    4. Dingli F,
    5. Loew D,
    6. Brahma S,
    7. Bartholomew B, and
    8. Papamichos-Chronakis M
    (2015) INO80 Chromatin Remodeler Facilitates Release of RNA Polymerase II from Chromatin for Ubiquitin-Mediated Proteasomal Degradation. Mol Cell 60:784–796 DOI: 10.1016/j.molcel.2015.10.028.
    OpenUrlCrossRefPubMed
  48. ↵
    1. Cortes Ledesma F,
    2. El Khamisy SF,
    3. Zuma MC,
    4. Osborn K, and
    5. Caldecott KW
    (2009) A human 5′-tyrosyl DNA phosphodiesterase that repairs topoisomerase-mediated DNA damage. Nature 461:674–678 DOI: 10.1038/nature08444.
    OpenUrlCrossRefPubMed
  49. ↵
    1. Lee KC,
    2. Bramley RL,
    3. Cowell IG,
    4. Jackson GH, and
    5. Austin CA
    (2016) Proteasomal inhibition potentiates drugs targeting DNA topoisomerase II. Biochem Pharmacol 103:29–39 DOI: 10.1016/j.bcp.2015.12.015.
    OpenUrl
  50. ↵
    1. Lee KC,
    2. Padget K,
    3. Curtis H,
    4. Cowell IG,
    5. Moiani D,
    6. Sondka Z,
    7. Morris NJ,
    8. Jackson GH,
    9. Cockell SJ,
    10. Tainer JA, et al.
    (2012) MRE11 facilitates the removal of human topoisomerase II complexes from genomic DNA. Biol Open 1:863–873 DOI: 10.1242/bio.20121834.
    OpenUrlAbstract/FREE Full Text
  51. ↵
    1. Lee KC,
    2. Swan RL,
    3. Sondka Z,
    4. Padget K,
    5. Cowell IG, and
    6. Austin CA
    (2018) Effect of TDP2 on the Level of TOP2-DNA Complexes and SUMOylated TOP2-DNA Complexes. Int J Mol Sci 19:2056 DOI: 10.3390/ijms19072056.
    OpenUrl
  52. ↵
    1. Li F,
    2. Raczynska JE,
    3. Chen Z, and
    4. Yu H
    (2019) Structural Insight into DNA-Dependent Activation of Human Metalloprotease Spartan. Cell Rep 26:3336–3346.e4 DOI: 10.1016/j.celrep.2019.02.082.
    OpenUrl
  53. ↵
    1. Liao S,
    2. Tammaro M, and
    3. Yan H
    (2016) The structure of ends determines the pathway choice and Mre11 nuclease dependency of DNA double-strand break repair. Nucleic Acids Res 44:5689–5701 DOI: 10.1093/nar/gkw274.
    OpenUrlCrossRefPubMed
  54. ↵
    1. Liu C-W and
    2. Jacobson AD
    (2013) Functions of the 19S complex in proteasomal degradation. Trends Biochem Sci 38:103–110 DOI: 10.1016/j.tibs.2012.11.009.
    OpenUrlCrossRefPubMed
  55. ↵
    1. Lopez-Mosqueda J,
    2. Maddi K,
    3. Prgomet S,
    4. Kalayil S,
    5. Marinovic-Terzic I,
    6. Terzic J, and
    7. Dikic I
    (2016) SPRTN is a mammalian DNA-binding metalloprotease that resolves DNA-protein crosslinks. eLife 5:e21491 DOI: 10.7554/eLife.21491.
    OpenUrl
  56. ↵
    1. Lou Z,
    2. Minter-Dykhouse K, and
    3. Chen J
    (2005) BRCA1 participates in DNA decatenation. Nat Struct Mol Biol 12:589–593 DOI: 10.1038/nsmb953.
    OpenUrlCrossRefPubMed
  57. ↵
    1. Maede Y,
    2. Shimizu H,
    3. Fukushima T,
    4. Kogame T,
    5. Nakamura T,
    6. Miki T,
    7. Takeda S,
    8. Pommier Y, and
    9. Murai J
    (2014) Differential and common DNA repair pathways for topoisomerase I- and II-targeted drugs in a genetic DT40 repair cell screen panel. Mol Cancer Ther 13:214–220 DOI: 10.1158/1535-7163.MCT-13-0551.
    OpenUrlAbstract/FREE Full Text
  58. ↵
    1. Mao Y,
    2. Desai SD, and
    3. Liu LF
    (2000) SUMO-1 conjugation to human DNA topoisomerase II isozymes. J Biol Chem 275:26066–26073 DOI: 10.1074/jbc.M001831200.
    OpenUrlAbstract/FREE Full Text
  59. ↵
    1. Mao Y,
    2. Desai SD,
    3. Ting C-Y,
    4. Hwang J, and
    5. Liu LF
    (2001) 26 S proteasome-mediated degradation of topoisomerase II cleavable complexes. J Biol Chem 276:40652–40658 DOI: 10.1074/jbc.M104009200.
    OpenUrlAbstract/FREE Full Text
  60. ↵
    1. Mårtensson S,
    2. Nygren J,
    3. Osheroff N, and
    4. Hammarsten O
    (2003) Activation of the DNA-dependent protein kinase by drug-induced and radiation-induced DNA strand breaks. Radiat Res 160:291–301 DOI: 10.1667/0033-7587(2003)160[0291:AOTDPK]2.0.CO;2.
    OpenUrlCrossRefPubMed
  61. ↵
    1. Maskey RS,
    2. Flatten KS,
    3. Sieben CJ,
    4. Peterson KL,
    5. Baker DJ,
    6. Nam H-J,
    7. Kim MS,
    8. Smyrk TC,
    9. Kojima Y,
    10. Machida Y, et al.
    (2017) Spartan deficiency causes accumulation of Topoisomerase 1 cleavage complexes and tumorigenesis. Nucleic Acids Res 45:4564–4576 DOI: 10.1093/nar/gkx107.
    OpenUrl
  62. ↵
    1. Milman N,
    2. Higuchi E, and
    3. Smith GR
    (2009) Meiotic DNA double-strand break repair requires two nucleases, MRN and Ctp1, to produce a single size class of Rec12 (Spo11)-oligonucleotide complexes. Mol Cell Biol 29:5998–6005 DOI: 10.1128/MCB.01127-09.
    OpenUrlAbstract/FREE Full Text
  63. ↵
    1. Moreau S,
    2. Ferguson JR, and
    3. Symington LS
    (1999) The nuclease activity of Mre11 is required for meiosis but not for mating type switching, end joining, or telomere maintenance. Mol Cell Biol 19:556–566 DOI: 10.1128/mcb.19.1.556.
    OpenUrlAbstract/FREE Full Text
  64. ↵
    1. Morimoto S,
    2. Tsuda M,
    3. Bunch H,
    4. Sasanuma H,
    5. Austin C, and
    6. Takeda S
    (2019) Type II DNA Topoisomerases Cause Spontaneous Double-Strand Breaks in Genomic DNA. Genes (Basel) 10:868 DOI: 10.3390/genes10110868.
    OpenUrl
  65. ↵
    1. Mosbech A,
    2. Gibbs-Seymour I,
    3. Kagias K,
    4. Thorslund T,
    5. Beli P,
    6. Povlsen L,
    7. Nielsen SV,
    8. Smedegaard S,
    9. Sedgwick G,
    10. Lukas C, et al.
    (2012) DVC1 (C1orf124) is a DNA damage-targeting p97 adaptor that promotes ubiquitin-dependent responses to replication blocks. Nat Struct Mol Biol 19:1084–1092 DOI: 10.1038/nsmb.2395.
    OpenUrlCrossRefPubMed
  66. ↵
    1. Muslimović A,
    2. Nyström S,
    3. Gao Y, and
    4. Hammarsten O
    (2009) Numerical analysis of etoposide induced DNA breaks. PLoS One 4:e5859 DOI: 10.1371/journal.pone.0005859.
    OpenUrlCrossRefPubMed
  67. ↵
    1. Nakamura K,
    2. Kogame T,
    3. Oshiumi H,
    4. Shinohara A,
    5. Sumitomo Y,
    6. Agama K,
    7. Pommier Y,
    8. Tsutsui KM,
    9. Tsutsui K,
    10. Hartsuiker E, et al.
    (2010) Collaborative action of Brca1 and CtIP in elimination of covalent modifications from double-strand breaks to facilitate subsequent break repair. PLoS Genet 6:e1000828 DOI: 10.1371/journal.pgen.1000828.
    OpenUrlCrossRefPubMed
  68. ↵
    1. Neale MJ,
    2. Pan J, and
    3. Keeney S
    (2005) Endonucleolytic processing of covalent protein-linked DNA double-strand breaks. Nature 436:1053–1057 DOI: 10.1038/nature03872.
    OpenUrlCrossRefPubMed
  69. ↵
    1. Nie M,
    2. Aslanian A,
    3. Prudden J,
    4. Heideker J,
    5. Vashisht AA,
    6. Wohlschlegel JA,
    7. Yates JR 3rd., and
    8. Boddy MN
    (2012) Dual recruitment of Cdc48 (p97)-Ufd1-Npl4 ubiquitin-selective segregase by small ubiquitin-like modifier protein (SUMO) and ubiquitin in SUMO-targeted ubiquitin ligase-mediated genome stability functions. J Biol Chem 287:29610–29619 DOI: 10.1074/jbc.M112.379768.
    OpenUrlAbstract/FREE Full Text
  70. ↵
    1. Olmedo-Pelayo J,
    2. Rubio-Contreras D, and
    3. Gómez-Herreros F
    (2020) Canonical non-homologous end-joining promotes genome mutagenesis and translocations induced by transcription-associated DNA topoisomerase 2 activity. Nucleic Acids Res 48:9147–9160 DOI: 10.1093/nar/gkaa640.
    OpenUrl
  71. ↵
    1. Paudyal SC,
    2. Li S,
    3. Yan H,
    4. Hunter T, and
    5. You Z
    (2017) Dna2 initiates resection at clean DNA double-strand breaks. Nucleic Acids Res 45:11766–11781 DOI: 10.1093/nar/gkx830.
    OpenUrlCrossRefPubMed
  72. ↵
    1. Paull TT,
    2. Rogakou EP,
    3. Yamazaki V,
    4. Kirchgessner CU,
    5. Gellert M, and
    6. Bonner WM
    (2000) A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage. Curr Biol 10:886–895 DOI: 10.1016/S0960-9822(00)00610-2.
    OpenUrlCrossRefPubMed
  73. ↵
    1. Quennet V,
    2. Beucher A,
    3. Barton O,
    4. Takeda S, and
    5. Löbrich M
    (2011) CtIP and MRN promote non-homologous end-joining of etoposide-induced DNA double-strand breaks in G1. Nucleic Acids Res 39:2144–2152 DOI: 10.1093/nar/gkq1175.
    OpenUrlCrossRefPubMed
  74. ↵
    1. Rao T,
    2. Gao R,
    3. Takada S,
    4. Al Abo M,
    5. Chen X,
    6. Walters KJ,
    7. Pommier Y, and
    8. Aihara H
    (2016) Novel TDP2-ubiquitin interactions and their importance for the repair of topoisomerase II-mediated DNA damage. Nucleic Acids Res 44:10201–10215 DOI: 10.1093/nar/gkw719.
    OpenUrlCrossRefPubMed
  75. ↵
    1. Rothenberg M,
    2. Kohli J, and
    3. Ludin K
    (2009) Ctp1 and the MRN-complex are required for endonucleolytic Rec12 removal with release of a single class of oligonucleotides in fission yeast. PLoS Genet 5:e1000722 DOI: 10.1371/journal.pgen.1000722.
    OpenUrlCrossRefPubMed
  76. ↵
    1. Salmena L,
    2. Lam V,
    3. McPherson JP, and
    4. Goldenberg GJ
    (2001) Role of proteasomal degradation in the cell cycle-dependent regulation of DNA topoisomerase IIalpha expression. Biochem Pharmacol 61:795–802 DOI: 10.1016/S0006-2952(01)00580-9.
    OpenUrlCrossRefPubMed
  77. ↵
    1. Sartori AA,
    2. Lukas C,
    3. Coates J,
    4. Mistrik M,
    5. Fu S,
    6. Bartek J,
    7. Baer R,
    8. Lukas J, and
    9. Jackson SP
    (2007) Human CtIP promotes DNA end resection. Nature 450:509–514 DOI: 10.1038/nature06337.
    OpenUrlCrossRefPubMed
  78. ↵
    1. Sasanuma H,
    2. Tsuda M,
    3. Morimoto S,
    4. Saha LK,
    5. Rahman MM,
    6. Kiyooka Y,
    7. Fujiike H,
    8. Cherniack AD,
    9. Itou J,
    10. Callen Moreu E, et al.
    (2018) BRCA1 ensures genome integrity by eliminating estrogen-induced pathological topoisomerase II-DNA complexes. Proc Natl Acad Sci USA 115:E10642–E10651 DOI: 10.1073/pnas.1803177115.
    OpenUrlAbstract/FREE Full Text
  79. ↵
    1. Schellenberg MJ,
    2. Appel CD,
    3. Adhikari S,
    4. Robertson PD,
    5. Ramsden DA, and
    6. Williams RS
    (2012) Mechanism of repair of 5′-topoisomerase II-DNA adducts by mammalian tyrosyl-DNA phosphodiesterase 2. Nat Struct Mol Biol 19:1363–1371 DOI: 10.1038/nsmb.2418.
    OpenUrlCrossRefPubMed
  80. ↵
    1. Schellenberg MJ,
    2. Appel CD,
    3. Riccio AA,
    4. Butler LR,
    5. Krahn JM,
    6. Liebermann JA,
    7. Cortés-Ledesma F, and
    8. Williams RS
    (2020) Ubiquitin stimulated reversal of topoisomerase 2 DNA-protein crosslinks by TDP2. Nucleic Acids Res 48:6310–6325 DOI: 10.1093/nar/gkaa318.
    OpenUrl
  81. ↵
    1. Schellenberg MJ,
    2. Lieberman JA,
    3. Herrero-Ruiz A,
    4. Butler LR,
    5. Williams JG,
    6. Muñoz-Cabello AM,
    7. Mueller GA,
    8. London RE,
    9. Cortés-Ledesma F, and
    10. Williams RS
    (2017) ZATT (ZNF451)-mediated resolution of topoisomerase 2 DNA-protein cross-links. Science 357:1412–1416 DOI: 10.1126/science.aam6468.
    OpenUrlAbstract/FREE Full Text
  82. ↵
    1. Schellenberg MJ,
    2. Perera L,
    3. Strom CN,
    4. Waters CA,
    5. Monian B,
    6. Appel CD,
    7. Vilas CK,
    8. Williams JG,
    9. Ramsden DA, and
    10. Williams RS
    (2016) Reversal of DNA damage induced Topoisomerase 2 DNA-protein crosslinks by Tdp2. Nucleic Acids Res 44:3829–3844 DOI: 10.1093/nar/gkw228.
    OpenUrlCrossRefPubMed
  83. ↵
    1. Sciascia N,
    2. Wu W,
    3. Zong D,
    4. Sun Y,
    5. Wong N,
    6. John S,
    7. Wangsa D,
    8. Ried T,
    9. Bunting SF,
    10. Pommier Y, et al.
    (2020) Suppressing proteasome mediated processing of topoisomerase II DNA-protein complexes preserves genome integrity. eLife 9:e53447 DOI: 10.7554/eLife.53447.
    OpenUrl
  84. ↵
    1. Sordet O,
    2. Larochelle S,
    3. Nicolas E,
    4. Stevens EV,
    5. Zhang C,
    6. Shokat KM,
    7. Fisher RP, and
    8. Pommier Y
    (2008) Hyperphosphorylation of RNA polymerase II in response to topoisomerase I cleavage complexes and its association with transcription- and BRCA1-dependent degradation of topoisomerase I. J Mol Biol 381:540–549 DOI: 10.1016/j.jmb.2008.06.028.
    OpenUrlCrossRefPubMed
  85. ↵
    1. Stingele J,
    2. Bellelli R,
    3. Alte F,
    4. Hewitt G,
    5. Sarek G,
    6. Maslen SL,
    7. Tsutakawa SE,
    8. Borg A,
    9. Kjær S,
    10. Tainer JA, et al.
    (2016) Mechanism and Regulation of DNA-Protein Crosslink Repair by the DNA-Dependent Metalloprotease SPRTN. Mol Cell 64:688–703 DOI: 10.1016/j.molcel.2016.09.031.
    OpenUrlCrossRefPubMed
  86. ↵
    1. Stingele J and
    2. Jentsch S
    (2015) DNA-protein crosslink repair. Nat Rev Mol Cell Biol 16:455–460 DOI: 10.1038/nrm4015.
    OpenUrlCrossRef
  87. ↵
    1. Stingele J,
    2. Schwarz MS,
    3. Bloemeke N,
    4. Wolf PG, and
    5. Jentsch S
    (2014) A DNA-dependent protease involved in DNA-protein crosslink repair. Cell 158:327–338 DOI: 10.1016/j.cell.2014.04.053.
    OpenUrlCrossRefPubMed
  88. ↵
    1. Sun Y,
    2. Jenkins LMM,
    3. Su YP,
    4. Nitiss KC,
    5. Nitiss JL, and
    6. Pommier Y
    (2019) A conserved SUMO-Ubiquitin pathway directed by RNF4/SLX5-SLX8 and PIAS4/SIZ1 drives proteasomal degradation of topoisomerase DNA-protein crosslinks. bioRxiv 707661. DOI: 10.1101/707661.
  89. ↵
    1. Sun Y,
    2. Saha LK,
    3. Saha S,
    4. Jo U, and
    5. Pommier Y
    (2020) Debulking of topoisomerase DNA-protein crosslinks (TOP-DPC) by the proteasome, non-proteasomal and non-proteolytic pathways. DNA Repair (Amst) 94:102926 DOI: 10.1016/j.dnarep.2020.102926.
    OpenUrl
  90. ↵
    1. Sunter NJ,
    2. Cowell IG,
    3. Willmore E,
    4. Watters GP, and
    5. Austin CA
    (2010) Role of Topoisomerase IIβ in DNA Damage Response following IR and Etoposide. J Nucleic Acids 2010:710589 DOI: 10.4061/2010/710589.
    OpenUrlCrossRefPubMed
  91. ↵
    1. Swan RL,
    2. Poh LLK,
    3. Cowell IG, and
    4. Austin CA
    (2020) Small Molecule inhibitors confirm ubiquitin-dependent removal of TOP2-DNA covalent complexes. Mol Pharmacol 98:222–233 DOI: 10.1124/mol.119.118893.
    OpenUrlAbstract/FREE Full Text
  92. ↵
    1. Swan RL,
    2. Cowell IG, and
    3. Austin CA
    (2021) A role for VCP/p97 in the processing of drug-stabilised TOP2-DNA covalent complexes. Mol Pharmacol 100:57-62 DOI: 10.1124/molpharm.121.000262.
  93. ↵
    1. Takeda S,
    2. Hoa NN, and
    3. Sasanuma H
    (2016) The role of the Mre11-Rad50-Nbs1 complex in double-strand break repair-facts and myths. J Radiat Res (Tokyo) 57 (Suppl 1):i25–i32 DOI: 10.1093/jrr/rrw034.
    OpenUrlCrossRefPubMed
  94. ↵
    1. Tammaro M,
    2. Barr P,
    3. Ricci B, and
    4. Yan H
    (2013) Replication-dependent and transcription-dependent mechanisms of DNA double-strand break induction by the topoisomerase 2-targeting drug etoposide. PLoS One 8:e79202 DOI: 10.1371/journal.pone.0079202.
    OpenUrlCrossRefPubMed
  95. ↵
    1. Tammaro M,
    2. Liao S,
    3. Beeharry N, and
    4. Yan H
    (2016) DNA double-strand breaks with 5′ adducts are efficiently channeled to the DNA2-mediated resection pathway. Nucleic Acids Res 44:221–231 DOI: 10.1093/nar/gkv969.
    OpenUrlCrossRefPubMed
  96. ↵
    1. van den Boom J,
    2. Wolf M,
    3. Weimann L,
    4. Schulze N,
    5. Li F,
    6. Kaschani F,
    7. Riemer A,
    8. Zierhut C,
    9. Kaiser M,
    10. Iliakis G, et al.
    (2016) VCP/p97 extracts sterically trapped Ku70/80 rings from DNA in double-strand break repair. Mol Cell 64:189–198 DOI: 10.1016/j.molcel.2016.08.037.
    OpenUrlCrossRefPubMed
  97. ↵
    1. Vaz B,
    2. Popovic M,
    3. Newman JA,
    4. Fielden J,
    5. Aitkenhead H,
    6. Halder S,
    7. Singh AN,
    8. Vendrell I,
    9. Fischer R,
    10. Torrecilla I, et al.
    (2016) Metalloprotease SPRTN/DVC1 orchestrates replication-coupled DNA-protein crosslink repair. Mol Cell 64:704–719 DOI: 10.1016/j.molcel.2016.09.032.
    OpenUrlCrossRefPubMed
  98. ↵
    1. Vaz B,
    2. Ruggiano A,
    3. Popovic M,
    4. Rodriguez-Berriguete G,
    5. Kilgas S,
    6. Singh AN,
    7. Higgins GS,
    8. Kiltie AE, and
    9. Ramadan K
    (2020) SPRTN protease and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability. bioRxiv DOI: 10.1101/2020.02.14.949289.
  99. ↵
    1. Verma R,
    2. Oania R,
    3. Fang R,
    4. Smith GT, and
    5. Deshaies RJ
    (2011) Cdc48/p97 mediates UV-dependent turnover of RNA Pol II. Mol Cell 41:82–92 DOI: 10.1016/j.molcel.2010.12.017.
    OpenUrlCrossRefPubMed
  100. ↵
    1. Wang W,
    2. Daley JM,
    3. Kwon Y,
    4. Krasner DS, and
    5. Sung P
    (2017) Plasticity of the Mre11-Rad50-Xrs2-Sae2 nuclease ensemble in the processing of DNA-bound obstacles. Genes Dev 31:2331–2336 DOI: 10.1101/gad.307900.117.
    OpenUrlAbstract/FREE Full Text
  101. ↵
    1. Wei Y,
    2. Diao L-X,
    3. Lu S,
    4. Wang H-T,
    5. Suo F,
    6. Dong M-Q, and
    7. Du L-L
    (2017) SUMO-Targeted DNA Translocase Rrp2 Protects the Genome from Top2-Induced DNA Damage. Mol Cell 66:581–596.e6 DOI: 10.1016/j.molcel.2017.04.017.
    OpenUrlCrossRef
  102. ↵
    1. Xiao H and
    2. Goodrich DW
    (2005) The retinoblastoma tumor suppressor protein is required for efficient processing and repair of trapped topoisomerase II-DNA-cleavable complexes. Oncogene 24:8105–8113 DOI: 10.1038/sj.onc.1208958.
    OpenUrlCrossRefPubMed
  103. ↵
    1. Xiao H,
    2. Mao Y,
    3. Desai SD,
    4. Zhou N,
    5. Ting C-Y,
    6. Hwang J, and
    7. Liu LF
    (2003) The topoisomerase IIbeta circular clamp arrests transcription and signals a 26S proteasome pathway. Proc Natl Acad Sci USA 100:3239–3244 DOI: 10.1073/pnas.0736401100.
    OpenUrlAbstract/FREE Full Text
  104. ↵
    1. Yan H,
    2. Tammaro M, and
    3. Liao S
    (2016) Collision of Trapped Topoisomerase 2 with Transcription and Replication: Generation and Repair of DNA Double-Strand Breaks with 5′ Adducts. Genes (Basel) 7:32 DOI: 10.3390/genes7070032.
    OpenUrl
  105. ↵
    1. Yun J,
    2. Kim Y-I,
    3. Tomida A, and
    4. Choi C-H
    (2009) Regulation of DNA topoisomerase IIalpha stability by the ECV ubiquitin ligase complex. Biochem Biophys Res Commun 389:5–9 DOI: 10.1016/j.bbrc.2009.08.066.
    OpenUrlPubMed
  106. ↵
    1. Zagnoli-Vieira G and
    2. Caldecott KW
    (2017) TDP2, TOP2, and SUMO: what is ZATT about? Cell Res 27:1405–1406 DOI: 10.1038/cr.2017.147.
    OpenUrl
  107. ↵
    1. Zeng Z,
    2. Cortés-Ledesma F,
    3. El Khamisy SF, and
    4. Caldecott KW
    (2011) TDP2/TTRAP is the major 5′-tyrosyl DNA phosphodiesterase activity in vertebrate cells and is critical for cellular resistance to topoisomerase II-induced DNA damage. J Biol Chem 286:403–409 DOI: 10.1074/jbc.M110.181016.
    OpenUrlAbstract/FREE Full Text
  108. ↵
    1. Zhang A,
    2. Lyu YL,
    3. Lin C-P,
    4. Zhou N,
    5. Azarova AM,
    6. Wood LM, and
    7. Liu LF
    (2006) A protease pathway for the repair of topoisomerase II-DNA covalent complexes. J Biol Chem 281:35997–36003 DOI: 10.1074/jbc.M604149200.
    OpenUrlAbstract/FREE Full Text
  109. ↵
    1. Zhao S,
    2. Kieser A,
    3. Li H-Y,
    4. Reinking HK,
    5. Weickert P,
    6. Euteneuer S,
    7. Yaneva D,
    8. Acampora AC,
    9. Götz MJ,
    10. Feederle R, et al.
    (2021) A ubiquitin switch controls autocatalytic inactivation of the DNA-protein crosslink repair protease SPRTN. Nucleic Acids Res 49:902–915 DOI: 10.1093/nar/gkaa1224.
    OpenUrl
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Molecular Pharmacology: 101 (1)
Molecular Pharmacology
Vol. 101, Issue 1
1 Jan 2022
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Review ArticleMinireview

Review of TOP2 Covalent Complex Repair Mechanisms

Rebecca L. Swan, Ian G. Cowell and Caroline A. Austin
Molecular Pharmacology January 1, 2022, 101 (1) 24-32; DOI: https://doi.org/10.1124/molpharm.121.000374

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Review ArticleMinireview

Review of TOP2 Covalent Complex Repair Mechanisms

Rebecca L. Swan, Ian G. Cowell and Caroline A. Austin
Molecular Pharmacology January 1, 2022, 101 (1) 24-32; DOI: https://doi.org/10.1124/molpharm.121.000374
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  • Article
    • Abstract
    • Introduction
    • DNA Topoisomerase II and TOP2 Poisons
    • Proteolytic Mechanisms
    • Tyrosyl DNA Phosphodiesterase 2 (TDP2)
    • Nucleolytic Mechanisms
    • Role of Post-Translational Modifications
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