Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Molecular Pharmacology
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Molecular Pharmacology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit molpharm on Facebook
  • Follow molpharm on Twitter
  • Follow molpharm on LinkedIn
Research ArticleArticle

Cholesterol Inhibition of Slo1 Channels Is Calcium-Dependent and Can Be Mediated by Either High-Affinity Calcium-Sensing Site in the Slo1 Cytosolic Tail

Kelsey C. North, Man Zhang, Aditya K. Singh, Dasha Zaytseva, Alexandria V. Slayden, Anna N. Bukiya and Alex M. Dopico
Molecular Pharmacology March 2022, 101 (3) 132-143; DOI: https://doi.org/10.1124/molpharm.121.000392
Kelsey C. North
Department of Pharmacology, Addiction Science, and Toxicology, College of Medicine, University of Tennessee Health Science Center, Memphis, Tennessee
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Man Zhang
Department of Pharmacology, Addiction Science, and Toxicology, College of Medicine, University of Tennessee Health Science Center, Memphis, Tennessee
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Aditya K. Singh
Department of Pharmacology, Addiction Science, and Toxicology, College of Medicine, University of Tennessee Health Science Center, Memphis, Tennessee
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dasha Zaytseva
Department of Pharmacology, Addiction Science, and Toxicology, College of Medicine, University of Tennessee Health Science Center, Memphis, Tennessee
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Alexandria V. Slayden
Department of Pharmacology, Addiction Science, and Toxicology, College of Medicine, University of Tennessee Health Science Center, Memphis, Tennessee
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Anna N. Bukiya
Department of Pharmacology, Addiction Science, and Toxicology, College of Medicine, University of Tennessee Health Science Center, Memphis, Tennessee
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Alex M. Dopico
Department of Pharmacology, Addiction Science, and Toxicology, College of Medicine, University of Tennessee Health Science Center, Memphis, Tennessee
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF + SI
  • PDF
Loading

Abstract

Calcium- and voltage-gated K+ channels of large conductance (BKs) are expressed in the cell membranes of all excitable tissues. Currents mediated by BK channel-forming slo1 homotetramers are consistently inhibited by increases in membrane cholesterol (CLR). The molecular mechanisms leading to this CLR action, however, remain unknown. Slo1 channels are activated by increases in calcium (Ca2+) nearby Ca2+-recognition sites in the slo1 cytosolic tail: one high-affinity and one low-affinity site locate to the regulator of conductance for K+ (RCK) 1 domain, whereas another high-affinity site locates within the RCK2 domain. Here, we first evaluated the crosstalking between Ca2+ and CLR on the function of slo1 (cbv1 isoform) channels reconstituted into planar lipid bilayers. CLR robustly reduced channel open probability while barely decreasing unitary current amplitude, with CLR maximal effects being observed at 10–30 µM internal Ca2+. CLR actions were not only modulated by internal Ca2+ levels but also disappeared in absence of this divalent. Moreover, in absence of Ca2+, BK channel-activating concentrations of magnesium (10 mM) did not support CLR action. Next, we evaluated CLR actions on channels where the different Ca2+-sensing sites present in the slo1 cytosolic domain became nonfunctional via mutagenesis. CLR still reduced the activity of low-affinity Ca2+ (RCK1:E379A, E404A) mutants. In contrast, CLR became inefficacious when both high-affinity Ca2+ sites were mutated (RCK1:D367A,D372A and RCK2:D899N,D900N,D901N,D902N,D903N), yet still was able to decrease the activity of each high-affinity site mutant. Therefore, BK channel inhibition by CLR selectively requires optimal levels of Ca2+ being recognized by either of the slo1 high-affinity Ca2+-sensing sites.

SIGNIFICANCE STATEMENT Results reveal that inhibition of calcium/voltage-gated K+ channel of large conductance (BK) (slo1) channels by membrane cholesterol requires a physiologically range of internal calcium (Ca2+) and is selectively linked to the two high-affinity Ca2+-sensing sites located in the cytosolic tail domain, which underscores that Ca2+ and cholesterol actions are allosterically coupled to the channel gate. Cholesterol modification of BK channel activity likely contributes to disruption of normal physiology by common health conditions that are triggered by disruption of cholesterol homeostasis.

Introduction

Calcium- and voltage-gated K+ channels of large conductance (MaxiK, BK) are widely distributed in animal tissues (Behrens et al., 2000; Orio et al., 2002). Upon activation, these channels generate outward K+ currents that control neuronal firing, smooth muscle contractility, endocrine secretion, circadian rhythm, and the processing of sensory information (Behrens et al., 2000; Orio et al., 2002; Pyott et al., 2007; Whitt et al., 2016; Dopico et al., 2018). Functional channels are formed by tetrameric association of channel-forming slo1 subunits (Salkoff et al., 2006). Slo1 monomers are modular proteins that combine pore-, voltage-, and calcium (Ca2+)-gating domains (Wang and Sigworth, 2009; Wu et al., 2010; Yuan et al., 2010; Giraldez and Rothberg, 2017) (Fig. 1). The intracellular cytosolic tail domain (CTD) includes two regulator of conductance for K+ structures (RCK1 and RCK2) (Xia et al., 2002). Each RCK contains a high-affinity Ca2+-binding site that senses nM-low μM Ca2+ (Zeng et al., 2005). In addition, RCK1 contains a low-affinity Ca2+-binding site that is sensitive to 0.1–100 mM Ca2+ (Zeng et al., 2005). Ca2+ binding to the homotetramer expands an elastic gating ring formed by a total of eight RCKs, which is coupled to the voltage-sensing domain through a rigid linker (Tao et al., 2017).

Fig. 1.
  • Download figure
  • Open in new tab
  • Download powerpoint
Fig. 1.

Schematic lateral view of the plasmalemma including four BK channel-forming slo1 proteins. Each slo1 (also known as BK channel α subunit) contains a voltage sensor domain, a central pore–gate domain, and a long C-terminus of intracellular location termed CTD. Each CTD contains two RCK domains. RCK1 (in blue) includes binding sites for Ca2+: a low-affinity Ca2+-sensing site (pink amino acid structures; highlighted by pink star) and a high-affinity Ca2+-sensing site (red amino acid structures; highlighted by red stars). The RCK2 domain (in orange) includes another high-affinity Ca2+-sensing site (green amino acid structures; highlighted by green star). Voltage sensor domain and CTD are connected to the pore–gate domain by linkers and through domain-domain interface contacts. The mutations conducted are presented according to the hslo1 amino acid sequence (Yuan et al., 2010) and color coded to the high- and low-affinity sites. Thus, slo1 mutations are: RCK1 high-affinity: D362A, D367A; RCK1 low-affinity: E374A, E399A; RCK2 high-affinity: D897A, D898A, D899A, D900A, D901A.

In most native tissues, BK channel-forming tetramers are accompanied by accessory subunits (β and γ-types) (Brenner et al., 2000; Orio et al., 2002; Yan and Aldrich, 2010; Yan and Aldrich, 2012). These small accessory proteins modulate pharmacological and biophysical properties of slo1, but do not generate current themselves (Brenner et al., 2000; Orio et al., 2002; Bukiya et al., 2009; Yan and Aldrich, 2010).

In turn, cholesterol (CLR) is an essential lipid in animals. At the organismal level, cholesterol serves as a precursor of steroid hormones and bile acids (Luu-The, 2013). Cellular effects of CLR include modulation of cell motility and organization of the actin cytoskeleton (Ramprasad et al., 2007), and the regulation of interactions between bacterial or viral (such as human immunodeficiency virus) pathogens and host cells (Goluszko and Nowicki, 2005; Schroeder and Cavacini, 2010). Within plasma membranes of animal cells, CLR plays the role of a structural lipid, providing an ordering effect on membrane phospholipids and modifying several physical properties of the lipid bilayer (Róg et al., 2009). Moreover, CLR has been increasingly recognized as an important regulator of the function of proteins, receptors in particular, including ion channels (Gimpl et al., 1997; Sooksawate and Simmonds, 2001; Addona et al., 2003; Levitan et al., 2010; Dopico et al., 2012).

CLR-induced regulation of BK-mediated ion currents has been widely reported, with both CLR-driven inhibition and activation of BK current being documented (reviewed in Dopico et al., 2012). Although the final effect of CLR on BK-mediated currents is influenced by the channel’s accessory subunits (Bukiya et al., 2021), it has been widely documented that exposure of homomeric slo1 to CLR levels found in membrane consistently decreases BK current (Crowley et al., 2003; Bukiya et al., 2011a; Singh et al., 2012). The molecular mechanisms leading to BK inhibition of slo1 remain largely unknown.

Modification of BK current by endogenous ligands or exogenous pharmacological agents is often influenced by activating levels of Ca2+ in the vicinity of the channel. Indeed, BK current potentiation by lithocholic acid, leukotriene B4, and ethyl alcohol are all modified by internal calcium present at the cytosolic/cis side of natural membranes/artificial lipid bilayers (Ca2+i) levels (Bukiya et al., 2007; Liu et al., 2008; Bukiya et al., 2014b). Moreover, ethyl alcohol, whose activation of slo1 is CLR-dependent (Bukiya et al., 2011a), is unable to increase slo1 activity in absence of this activating divalent. Based on these precedents, here we used lipid bilayers to test the hypothesis that BK current inhibition by CLR is Ca2+-dependent and, if so, determined by specific Ca2+-sensing sites within the slo1 protein. Our results prove this hypothesis by revealing an optimal Ca2+i level for CLR-induced inhibition of slo1 currents, an effect that involves major reduction in channel steady-state activity and minor reduction in unitary conductance. Moreover, using engineered slo1s that make one or more Ca2+-sensing sites nonfunctional, we demonstrate that as far as Ca2+i reaches levels to optimally activate the channel via either of the two high-affinity Ca2+ sites located in the CTD, CLR inhibits channel activity. This CLR action, however, is lost when the channel is solely activated via its low affinity site Ca2+ site also present in the slo1 CTD. Our study reveals the molecular mechanism of CLR-induced slo1 channel inhibition and, thus, expands our understanding of CLR effects on ion channels dually gated by ligands and transmembrane voltage.

Materials and Methods

Preparation of Slo1 Protein

BK channel-forming α subunit cDNA (cbv1; AY330293) cloned from rat cerebral artery myocytes (Jaggar et al., 2005) was inserted into the pcDNA3.1 plasmid vector as described (Singh et al., 2012). Ca2+-sensing site mutants were introduced into cDNAs coding for cbv1 inserted into the pBluescript as described (Liu et al., 2008). The presence of desired nucleotide substitutions and absence of unwanted mutations were verified by automatic sequencing at the Molecular Research Center of the University of Tennessee Health Science Center. Human embryonic kidney 293 cells were transiently transfected with slo1-carrying pcDNA3.1 plasmid using Lipofectamine 2000 (Invitrogen, Carlsbad, CA). Mutated slo1-carrying pBluescript plasmids were transfected into Chinese hamster ovary cell using Lipofectamine 2000 (Invitrogen, Carlsbad, CA). Transfected cell lines were grown to confluence, pelleted, and resuspended on ice in 10 ml of buffer solution of the following composition (mM): 30 KCl, 2 MgCl2, 10 HEPES, 5 EGTA; pH 7.2. A membrane preparation was obtained using a sucrose gradient as previously described (Crowley et al., 2003). Aliquots were stored at −80°C.

Recording of Ionic Current after Slo1 Reconstitution into Planar Lipid Bilayers

CLR was dissolved in chloroform and then introduced into a lipid mixture of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-l-serine (POPS) 3:1 (w/w) to render a final CLR concentration of 20% w/w (33 mol%). Lipid mixtures, whether containing CLR or not, were dried under nitrogen (N2) gas and resuspended in 25 mg/ml of n-decane. Vertical bilayers (80–120 picofarads) were formed by painting the lipid mix across a 200 μm diameter hole in a deldrin cup (Warner Instruments). The membrane preparation of cbv1 protein was added to the cis chamber. Fusion between the membrane preparation and the bilayer was promoted by osmosis, with the cis chamber recording solution being hyperosmotic to the trans chamber solution. In all recordings, the cis and trans chambers were set as the intracellular and extracellular compartments, respectively. Solution for the cis chamber included (mM): 300 KCl, 10 HEPES; pH 7.2. Solution for the trans chamber included (mM): 30 KCl, 10 HEPES; pH 7.2. In all experiments, free Ca2+ in cis and trans chamber solutions was the same, with free Ca2+ being adjusted to the desired value by adding calcium dichloride (CaCl2) and EGTA into the cis and trans chamber solutions. For the experiments with Ca2+≥1 μM, HEDTA was also added. In Ca2+-free solution, CaCl2 was omitted. Nominal free Ca2+ in solution was calculated using the MaxChelator Sliders program (C. Patton, Stanford University) and validated experimentally using a Ca2+-selective electrode (Cole-Parmer) (Dopico, 2003). The trans chamber was connected to ground, whereas the cis chamber voltage was clamped at potentials relative to ground. Only cbv1 with its Ca2+-sensors oriented toward the cis chamber was used for data acquisition and analysis.

Ionic currents were acquired during 20–30 seconds of continuous recording at various voltages using a Warner BC-525D amplifier, low passed-filtered at 1 kHz using the 4-pole Bessel filter built into the amplifier and sampled at 5 kHz with Digidata 1322A/pCLAMP 8 (Molecular Devices). Data from mutated cbv1 were acquired using identical parameters yet sampled using Digidata 1550B/pClamp10. For proper comparisons with previous data obtained by us (Crowley et al., 2003; Bukiya et al., 2008 ; 2011a ; 2011b) and others (Chang et al., 1995; Yuan et al., 2007), studies were conducted at room temperature (20–25°C). We used NPo as an index of channel steady-state activity, where N = number of functional channels present in the bilayer and Po = individual channel opening probability. At the end of the experiment, channels were exposed to high (≫100 µM) Ca2+ solutions and positive voltage (70 mV) at the cis side of the bilayer. Under these conditions, slo1 Po approaches 1 and thus an approximation of N can be obtained from the maximal number of overlapping opening levels, which led us to calculate Po from the computed NPo. NPo and unitary current amplitude were computed using a built-in routine in Clampfit 10.6 (Molecular Devices, San Jose, CA) using a 50% threshold for event detection. For the calculation of Vhalf, that is, the voltage at which (N)Po/(N)Pomax reached 0.5 (half-maximal activity), (N)Po/(N)Pomax-V plots were created fitting data to a Boltzmann function using a fitting routine in Origin 8.5.1 (Originlab).

Chemicals

CLR, POPS (sodium salt), and POPE were purchased from Avanti Polar Lipids (Alabaster, AL). All other chemicals and reagents were purchased from Sigma-Aldrich (St. Louis, MO).

Statistical Analysis

Analysis was conducted using InStat 3.0 (GraphPad, San Diego, CA). Groups of data are shown as mean ±S.D. Normal distribution of data were determined by the Kolmogorov-Smirnov’s test. A set of channel recordings under a given experimental condition (e.g., CLR-free) throughout the entire voltage range from a single bilayer was considered a single experimental observation. When the number of observations ≥10 and data distribution was Gaussian, unpaired Student’s t test (one-tail) was used to determine the statistical significance between individual means. However, when number of observations ≤10 or data distribution was not Gaussian, Mann-Whitney test (one-tail) was used. Comparisons of data, whether Po or unitary current amplitude, between CLR-containing versus CLR-free bilayers were conducted for each specific voltage. Vhalfs and fitting data to Boltzmann functions were determined for each bilayer recorded, with data being acquired throughout the entire range of voltages.

Results

In the Absence of Ca2+i CLR Fails to Significantly Inhibit Slo1-Mediated Currents

We first set to determine whether the presence of the slo1 (cbv1) physiologic activating ligand (Ca2+) was critical for CLR to inhibit channel function. The basic BK phenotype of cbv1-mediated currents in this system was established as previously described (Bukiya et al., 2011a,b). The CLR amount used corresponds to a molar fraction of 0.33 (i.e., 33 mol%), which is within the CLR range found in the plasma membrane of animal cells in most tissues (30–50 mol%; Gennis, 1989). The CLR molar fraction used also corresponds to the CLR concentration that reduces the steady state activity (NPo) of human brain hslo1 subunits (Crowley et al., 2003) and cbv1 itself (Bukiya et al., 2008) when reconstituted into binary phospholipid bilayers identical to those used in the current experiments. After cbv1 incorporation, we perfused both the cis and trans sides of the bilayer with Ca2+-free solution, i.e., a high K+ solution that contained only trace amounts of Ca2+ (∼0.5 nM; Cox and Aldrich, 2000). Channel activity was then recorded at a wide range of transbilayer voltage (−60 to +80 mV). More extreme voltages, whether of positive or negative polarity, resulted in unstable bilayers that, under our experimental conditions, consistently broke within a few seconds after formation.

CLR failed to modify cbv1 Po in the absence of activating Ca2+ (Fig. 2, A and B), a result that was replicated in all bilayers (n = 7 for controls; n = 6 for CLR-containing bilayers). The extremely low activity of cbv1 in absence of activating Ca2+, as found for other slo1 (Cox and Aldrich, 2000), prevented us from experimentally determining maximal cbv1 activity and thus obtain Po/Pomax plots straightforwardly. However, previous reports from our laboratory and others estimated 300–400 mV as the voltage range at which slo1s, including cbv1, reached maximal steady-steady activity (Vmax) in absence of Ca2+ (Cox and Aldrich, 2000; Horrigan and Aldrich, 2002; Kuntamallappanavar and Dopico, 2016). Using these Vmax values, we conducted a Boltzmann fitting of our current data to estimate cbv1 current Vhalf: 138.28 ±14.72 and 138.94 ±26.16 mV in control and CLR-containing bilayers, respectively. The values obtained in control bilayers are similar to those previously reported for slo1 other than cbv1 (Cox and Aldrich, 2000; Horrigan and Aldrich, 2002) and cbv1 itself (Kuntamallappanavar and Dopico, 2016) under these Ca2+-free conditions. Thus, present data demonstrate that CLR fails to significantly reduce cbv1 steady-state activity in the absence of activating Ca2+I, that is, when slo1 activity is driven by voltage- and/or intrinsic-gating (i.e., closed to open transitions in absence of Ca2+i and with the voltage sensors largely dwelling in their resting state; Cui et al., 1997; Horrigan and Aldrich, 1999).

Fig. 2.
  • Download figure
  • Open in new tab
  • Download powerpoint
Fig. 2.

CLR fails to inhibit slo1 channel in absence of Ca2+i or at low micromolar Ca2+i. (A) Representative channel recordings obtained after incorporation of slo1 into CLR-free (left column) versus CLR-containing (right column) POPE/POPS (3:1 wt/wt) bilayers bathed by Ca2+-free bilayer recording solutions. Channel openings are shown as upward deflections; arrows indicate the baseline level (all channels closed). (B) Averaged data showing open probability (Po) as a function of transmembrane voltage in CLR-free versus CLR-containing bilayers; data are shown as mean ±standard deviation. (C) Scattered data showing unitary current amplitude values in CLR-free versus CLR-containing bilayers at varying transmembrane voltages; data are shown as mean ±standard deviation. (D) Representative channel recordings obtained after incorporation of slo1 into CLR-free (left column) versus CLR-containing (right column) POPE/POPS (3:1 wt/wt) bilayers bathed by bilayer recording solution containing 1 μM free Ca2+. Channel openings are shown as upward deflections; arrows indicate the baseline level (channels closed). (E) Averaged data showing Po as a function of transmembrane voltage in CLR-free versus CLR-containing bilayers; data are shown as mean ±standard deviation. (F) Scattered data showing unitary current amplitude values in CLR-free versus CLR-containing bilayers at varying transmembrane voltages; data are shown as mean ±standard deviation.

In addition to its failure to modify steady-state activity, CLR failed to modify cbv1 unitary current amplitude (i) when evaluated in the absence of Ca2+ (Fig. 2C). Total ionic current due to cbv1 expression in a bilayer is determined by N, Po, and i. Given the lack of CLR action on i and NPo, it is possible to conclude that in the absence of Ca2+, CLR fails to significantly modify the total ionic current mediated by cbv1.

Cholesterol-Induced Inhibition of Slo1 Currents Requires a Specific Range of Ca2+ Levels

As Ca2+i reaches µM levels, modification of Ca2+-driven gating processes more effectively translates into changes in BK Po (Meera et al., 1996; Cui et al., 1997; Rothberg and Magleby, 2000). At these levels, Ca2+ begins to be sensed by the two high-affinity Ca2+-recognition sites present in the slo1 CTD, opening the octameric gating ring and leading to increased Po (Hite et al., 2017). At 1 μM Ca2+i, however, slo1 Po and unitary current amplitudes in CLR-containing bilayers still remained basically unmodified from their counterparts in CLR-free bilayers (Fig. 2, D–F). Vhalfs reached 139.05 ±38.74 and 112.28 ±16.93 mV in CLR-containing versus control bilayers, respectively. Remarkably, in Ca2+-free systems, exposure of slo1 channels to 10 mM magnesium at the intracellular side of the bilayer (Mg2+i), i.e., a Mg2+i concentration that activates BK channels on its own (i.e., independently of Ca2+i) failed to endow the slo1 channel with sensitivity to CLR inhibitory effect (Supplemental Fig. 2). Data indicate that the dependence of CLR inhibition of slo1 channels on activating Ca2+i does not generalize to include another physiologic divalent and suggest the existence of a rather selective coupling between CLR- and Ca2+-sensing elements in the slo1 protein.

As Ca2+i was further increased to 10 μM, the effects of CLR on slo1 function became clearly evident: CLR was able to significantly (P < 0.05) decrease cbv1 activity within a window of transmembrane voltages from −20 to +30 mV (Fig. 3, A and B). CLR-induced cbv1 inhibition at 10 μM Ca2+i translated into a significant increase in Vhalf; from −3.88 ±22.95 to 22.57 ±35.90 mV (Fig. 3, C and D). Unitary current amplitude, however, remained basically unchanged by CLR (Fig. 3E), consistent with previous findings that have reported minor, if any, decrease in unitary conductance of native or recombinant BK channels in response to CLR molar fractions that drastically decrease steady-state activity (reviewed in Dopico et al., 2012). CLR-induced slo1 inhibition became even more significant as Ca2+i was increased to 30 μM (Fig. 3F). At this Ca2+i, the CLR-induced decrease in cbv1 Po became more robust reaching significance across a wider voltage-range (from −50 to +30 mV) (Fig. 3G). Thus, CLR evoked a parallel, rightward shift in Po/Pomax-V curve along the voltage axis (Fig. 3H), which translated into a significant increase in Vhalf: from −27.10 ±24.91 to 3.54 ±10.72 mV (Fig. 3I). Although CLR effect on unitary current amplitude at 30 μM free Ca2+ did not reach significance, P values of comparison between CLR-free and CLR-containing bilayers were consistently between 0.08–0.05. Thus, had the sample size been larger, the CLR-evoked minor decrease in unitary current amplitude under these conditions would have likely reached significance (Fig. 3J). Moreover, titration of CLR in the bilayer led to modification of slo1 channel function in a concentration-dependent manner: both unitary current amplitude and Po were barely affected by 10 or 20 mol% CLR, significantly reduced by 33 mol% (see above), and further reduced by 45 mol% (Supplemental Fig. 1).

Fig. 3.
  • Download figure
  • Open in new tab
  • Download powerpoint
Fig. 3.

CLR-induced slo1 channel inhibition in presence of 10 and 30 μM Ca2+. (A) Representative channel recordings obtained after incorporation of slo1 into CLR-free (left column, two channels are incorporated) versus CLR-containing (right column, one channel is incorporated) POPE/POPS (3:1 wt/wt) bilayers. Bilayers are bathed by recording solutions with 10 μM free Ca2+. Channel openings are shown as upward deflections; arrows indicate the baseline level (channels closed). (B) Averaged data showing Po as a function of transmembrane voltage in CLR-free versus CLR-containing bilayers; data are shown as mean ±standard deviation. *Statistically significant difference from CLR-free bilayers, P < 0.05 by a one-tail Mann-Whitney test. C. Averaged data showing Po/Pomax as a function of transmembrane voltage in CLR-free versus CLR-containing bilayers. D. Scattered data showing Vhalfs of individual bilayers; data are shown as mean ±standard deviation. *Statistically significant difference from CLR-free bilayers, P = 0.0465 by a one-tail t test. (E) Scattered data showing unitary current amplitude values in CLR-free versus CLR-containing bilayers at varying transmembrane voltages at 10 μM free Ca2+; data are shown as mean ±standard deviation. (F) Representative channel recordings obtained after incorporation of slo1 into CLR-free (left column, two channels are incorporated) versus CLR-containing (right column, three channels are incorporated) POPE/POPS (3:1 wt/wt) bilayers bathed by bilayer recording solutions with 30 μM free Ca2+. Channel openings are shown as upward deflections; arrows indicate the baseline level (channels closed). (G) Averaged data showing Po as a function of transmembrane voltage in CLR-free versus CLR-containing bilayers; data are shown as mean ±standard deviation. *Statistically significant difference from CLR-free bilayers, 0.010 < P < 0.050; #Statistically significant difference from CLR-free bilayers, 0.003 < P < 0.010 (one-tail Mann-Whitney test). (H) Averaged data showing Po/Pomax as a function of transmembrane voltage in CLR-free versus CLR-containing bilayers; data are shown as mean ±standard deviation. (I) Scattered data showing Vhalfs of individual bilayers; data are shown as mean ±standard deviation. #Statistically significant difference from CLR-free bilayers, P = 0.0055 by a one-tail Mann-Whitney test. (J) Scattered data showing unitary current amplitude values in cholesterol-free versus cholesterol-containing bilayers at varying transmembrane voltages; data are shown as mean ±standard deviation.

It is noteworthy that the robust inhibition of slo1 channel activity evoked in the presence of 30 µM Ca2+i was identical in the absence and presence of activating Mg2+i (10 mM Mg2+i; Supplemental Fig. 3), underscoring a lack of synergism between the two physiologic divalents on CLR inhibition of slo1 channels and the selectivity in the coupling of CLR-sensing to Ca2+-driven gating.

We next evaluated the CLR response of cbv1 at even higher Ca2+ levels (≥100 µM). These levels are above those needed to fully activate the slo1’s two high-affinity Ca2+-sensing sites while able to begin to activate these channels through a low affinity Ca2+i-sensing site (Zeng et al., 2005). At 100 μM Ca2+i, CLR somewhat decreased cbv1 Po, yet this effect was significant within a voltage range (from −20 to +20 mV) (Fig. 4, A and B) narrower than that observed at 10–30 µM Ca2+i. As CLR did not evoke a substantial shift in the Po/Pomax-V relationship along the x-axis (Fig. 4C) Vhalfs the difference in Vhalf between CLR-free and CLR-containing was not significant, reaching 42.04 ±4.94 and 34.57 ±12.38 mV, respectively (Fig. 4D). In turn, a mild decrease in unitary current amplitude in CLR-containing bilayers was detected (Fig. 4E).

Fig. 4.
  • Download figure
  • Open in new tab
  • Download powerpoint
Fig. 4.

CLR-induced slo1 channel inhibition in presence of 100 and 850 μM Ca2+. (A) Representative channel recordings obtained after incorporation of cbv1 into CLR-free (left column, two channels are incorporated) versus CLR-containing (right column, one channel is incorporated) POPE/POPS (3:1 wt/wt) bilayers bathed by bilayer recording solutions with 100 μM free Ca2+. Channel openings are shown as upward deflections; arrows indicate the baseline level (channels closed). (B) Averaged data showing Po as a function of transmembrane voltage in CLR-free versus CLR-containing bilayers at 100 μM free Ca2+; data are shown as mean ±standard deviation. *Statistically significant difference from CLR-free bilayers, P = 0.036; #Statistically significant difference from CLR-free bilayers, 0.0003 < P < 0.010 (one-tail Mann-Whitney test). (C) Averaged data showing Po/Pomax as a function of transmembrane voltage in CLR-free versus CLR-containing bilayers at 100 μM free Ca2+; data are shown as mean ±standard deviation. D. Scattered data showing Vhalfs of individual bilayers at 100 μM free Ca2+. (E) Scattered unitary current amplitude values in CLR-free versus CLR-containing bilayers at varying transmembrane voltages at 100 μM free Ca2+; data are shown as mean ±standard deviation. *Statistically significant difference from CLR-free bilayers, 0.010 < P < 0.050; #Statistically significant difference from CLR-free bilayers, 0.007 < P < 0.010 (one-tail Mann-Whitney test). (F) Representative channel recordings obtained after incorporation of slo1 into CLR-free (left column, three channels are incorporated) versus CLR-containing (right column, one channel is incorporated) POPE/POPS (3:1 wt/wt) bilayers bathed by bilayer recording solutions with 850 μM free Ca2+. Channel openings are shown as upward deflections; arrows indicate the baseline level (channels closed). (G) Averaged data showing Po as a function of transmembrane voltage in CLR-free versus CLR-containing bilayers; data are shown as mean ±standard deviation. *Statistically significant difference from CLR-free bilayers, 0.015 < P < 0.050; #Statistically significant difference from CLR-free bilayers, 0.008 < P < 0.010 (one-tail Mann-Whitney test). (H) Averaged data showing Po/Pomax as a function of transmembrane voltage in CLR-free versus CLR-containing bilayers; data are shown as mean ±standard deviation. (I) Scattered data showing Vhalfs of individual bilayers; data are shown as mean ±standard deviation. (J) Scattered unitary current amplitude values in CLR-free versus CLR-containing bilayers at varying transmembrane voltages; data are shown as mean ±standard deviation.

Finally, increasing Ca2+ levels to 850 μM sustained the CLR-driven decrease in slo1 Po, only between −40 and 0 mV (Fig. 4, F–G). At this high level of Ca2+, CLR action did not result in a significant shift of the Po/Pomax-V curve (Fig. 4H); averaged Vhalf values were very close, reaching −46.34 ±12.61 and −39.85 ±24.43 mV in CLR-free and CLR-containing bilayers, respectively (Fig. 4I). In addition, the CLR-induced decrease in unitary current amplitude that was observed within 1–100 μM Ca2+ was lost (Fig. 4, E versus J). Collectively, our evaluation of CLR action on cbv1 function within a wide Ca2+ range (0–850 µM) shows that under the extreme points of this range CLR-induced reduction of cbv1 Po (robust) and i (mild) are lost, whereas CLR action is maximal at the Ca2+i range of 10–100 µM (Fig. 5).

Fig. 5.
  • Download figure
  • Open in new tab
  • Download powerpoint
Fig. 5.

CLR-induced inhibition of BK channels requires specific Ca2+ levels. Averaged data showing Ca2+ dependency of CLR-driven increase in Vhalf; data are shown as mean ±standard deviation. *Statistically significant difference from CLR-free bilayers, P = 0.0465 by a one-tail t test; #Statistically significant difference from CLR-free bilayers, P = 0.0055 by a one-tail Mann-Whitney test.

CLR-Induced cbv1 Inhibition Is Mediated by the High-Affinity Ca2+-Sensing Sites Located in the Channel Subunit Cytosolic Tail Domain

To begin to understand the structural basis of Ca2+i-CLR interactions on slo1 activity, we evaluated the effects of CLR on the activity of cbv1s in which their Ca2+-sensing site(s) were made nonfunctional by point mutagenesis and in the presence of Ca2+i levels that fully activated/saturated the site under analysis. Thus, CLR significantly decreased the NPo of the low-affinity RCK1 (E379A, E404A) mutant when evaluated at 30 μM Ca2+i (Fig. 6). In contrast, CLR-driven decrease in Po was lost in a construct with both high-affinity sites for Ca2+i being nonfunctional (RCK1:D367A, D372A, and RCK2:D899N, D900N, D901N, D902N, D903N) when recorded at 10 mM Ca2+i, a concentration fully able to activate slo1 through their low-affinity RCK1 site (Zeng et al., 2005). There was no significant CLR-induced decrease in unitary current amplitude detected in either the low- or high-affinity site cbv1 mutants. Notably, CLR was still able to decrease the NPo and unitary current amplitude of each individual high-affinity site mutant, i.e., RCK1:D367A, D372A, and RCK2:D899N, D900N, D901N, D902N, D903N when evaluated at 30 µM Ca2+i (Fig. 7). Collectively, evaluation of CLR action on these constructs indicates that CLR inhibition of cbv1 is coupled to activating levels of Ca2+i being recognized by either of the two high-affinity Ca2+i-sensing sites present in the cbv1 CTD.

Fig. 6.
  • Download figure
  • Open in new tab
  • Download powerpoint
Fig. 6.

(A) Representative channel recordings obtained after incorporation of the low-affinity Ca2+ mutant, (RCK1: E379A, E404A) into CLR-free (left column, three channels are incorporated) versus CLR-containing (right column, two channels are incorporated) POPE/POPS (3:1 wt/wt) bilayers bathed by bilayer recording solutions with 30 μM free Ca2+. Channel openings are shown as upward deflections; arrows indicate the baseline level (channels closed). (B) Averaged data showing Po as a function of transmembrane voltage in CLR-free versus CLR-containing bilayers at 30 μM free Ca2+; data are shown as mean ±standard deviation. *Statistically significant difference from CLR-free bilayers, P = 0.04; P = 0.039 (one-tail Mann-Whitney test). (C) Scattered unitary current amplitude values in CLR-free versus CLR-containing bilayers at varying transmembrane voltages at 30 μM free Ca2+; data are shown as mean ±standard deviation. (D) Representative channel recordings obtained after incorporation of both high-affinity Ca2+ site mutant (RCK1:D367A, D372A, and RCK2:D899N, D900N, D901N, D902N, D903N) into CLR-free (left column, two channels are incorporated) versus CLR-containing (right column, two channels are incorporated) POPE/POPS (3:1 wt/wt) bilayers bathed by bilayer recording solutions with 10 mM free Ca2+. Channel openings are shown as upward deflections; arrows indicate the baseline level (channels closed). (E) Averaged data showing Po as a function of transmembrane voltage in CLR-free versus CLR-containing bilayers; data are shown as mean ±standard deviation. (F) Scattered unitary current amplitude values in CLR-free versus CLR-containing bilayers at varying transmembrane voltages; data are shown as mean ±standard deviation.

Fig. 7.
  • Download figure
  • Open in new tab
  • Download powerpoint
Fig. 7.

(A) Representative channel recordings obtained after incorporation of the RCK1 high-affinity Ca2+ site mutant (RCK1: D367A, D372A) into CLR-free (left column, two channels are incorporated) versus CLR-containing (right column, two channels are incorporated) POPE/POPS (3:1 wt/wt) bilayers bathed by bilayer recording solutions with 30 μM free Ca2+. Channel openings are shown as upward deflections; arrows indicate the baseline level (channels closed). (B) Averaged data showing Po as a function of transmembrane voltage in CLR-free versus CLR-containing bilayers at 30 μM free Ca2+; data are shown as mean ±standard deviation. *Statistically significant difference from CLR-free bilayers, P = 0.045; P = 0.030; P = 0.024; P = 0.019 (one-tail Mann-Whitney test). (C) Scattered unitary current amplitude values in CLR-free versus CLR-containing bilayers at varying transmembrane voltages at 30 μM free Ca2+; data are shown as mean ±standard deviation. *Statistically significant difference from CLR-free bilayers, P = 0.003, P = 0.011, P = 0.021, P = 0.027, P = 0.044. (D) Representative channel recordings obtained after incorporation the RCK2 high-affinity Ca2+ site mutant (D899N, D900N, D901N, D902N, D903N) into CLR-free (left column, one channel is incorporated) versus CLR-containing (right column, one channel is incorporated) POPE/POPS (3:1 wt/wt) bilayers bathed by bilayer recording solutions with 30 μM free Ca2+. Channel openings are shown as upward deflections; arrows indicate the baseline level (channels closed). (E) Averaged data showing Po as a function of transmembrane voltage in CLR-free versus CLR-containing bilayers; data are shown as mean ±standard deviation. *Statistically significant difference from CLR-free bilayers, P = 0.029, P = 0.038. (F) Scattered unitary current amplitude values in CLR-free versus CLR-containing bilayers at varying transmembrane voltages; data are shown as mean ±standard deviation. *Statistically significant difference from CLR-free bilayers, P = 0.02, P = 0.015.

Discussion

Present data demonstrate that CLR inhibition of cbv1 is selectively coupled to Ca2+i-driven gating; when cbv1 activity is driven by intrinsic/voltage-gating, CLR becomes ineffective. Moreover, there is a narrow range of Ca2+i (10–30 μM) at which (a) this divalent fully activates slo1 through its Ca2+-sensing high-affinity sites (Bao et al., 2002 ; 2004) and (b) CLR action is more robust and reaches significance across a wider voltage range. Robustness in CLR inhibition of slo1 channels within this narrow Ca2+i range likely explains the previously reported high efficacy of 33 mol% CLR to reduce the Po of hslo1 (cloned from human brain; Crowley et al., 2003) and cbv1 themselves (Bukiya et al., 2011a) when expressed in a similar binary bilayer, as data were obtained at 10 and 50 µM Ca2+i. Current data also show that CLR modulation of gating is lost when Ca2+i gets close to 1 mM (850 μM), i.e., levels at which the high-affinity sites are saturated but cbv1 activation by Ca2+i can still be evoked by the low-affinity site present in the CTD (Zhang et al., 2001). Consistent with the lack of involvement of this site in CLR action, 10 mM Mg2+i neither endows slo1 channels with CLR sensitivity in the absence of Ca2+i (Supplemental Fig. 2) nor modifies CLR action in the presence of optimal levels of Ca2+i (30 μM) (Supplemental Fig. 3). Moreover, data from engineered slo1 reveal that CLR action, as reported for ethanol (Liu et al., 2008), involves either of the high-affinity Ca2+-sensing sites being optimally activated by Ca2+i. Of note, although the RCK1 high-affinity site is much closer to the pore-gate region than RCK2, the logarithmic relationships between Vhalf and activating Ca2+i suggest that the electrical distances between the binding sites (and thus their functional coupling to the gate) are equivalent (Cui et al., 1997). Remarkably, both high-affinity sites are surrounded by hydrophobic residues on which CLR can potentially dock. In particular, Tyr450, the central tyrosine in one of the several cholesterol consensus domains (CRAC) found in the CTD that participate in CLR-sensing by slo1 (Singh et al., 2012) is located within the flexible interface between RCK1 and RCK2 (see below).

The requirement for an optimal Ca2+ level to enable CLR-induced cbv1 inhibition is open to several interpretations. One is that Ca2+i binding promotes a cbv1 conformation that enables access and accommodation of CLR into a CLR binding site(s). This role for Ca2+, i.e., enabling accommodation of a modulatory agent into a “newly available” recognition site in the slo1 CTD has been demonstrated for ethanol and related n-alkanols. These alcohols dock onto a water-accessible site within a flexible interface positioned between the Ca2+-sensors and the gate. When Ca2+ is bound, the ethanol site between Lys361 and Arg514 is revealed. In the Ca2+-unbound state, however, spatial reorientation repositions Met909, which now prevents the ethanol molecule from accessing Lys361 for hydrogen bonding and eventual alcohol-induced increase in Po (Bukiya et al., 2014a). This ethanol-CLR analogy is particularly appealing as modulation of slo1 by these ligands shares many features: (1) minor, if any, effects on ion conduction at ligand concentrations that drastically change Po; (2) need of Ca2+ presence for ligand action; and (3) coupling of ligand action to Ca2+ interaction with any of the two high-affinity, but not the low-affinity, Ca2+-sensing sites in the CTD (current data and Liu et al., 2008).

In a second scenario, CLR could still bind to slo1 in absence of Ca2+i, yet the expansion of the gating ring driven by Ca2+-binding that eventually leads to channel opening (Yuan et al., 2011; Hite et al., 2017; Jia et al., 2018) should introduce a conformational change that allows CLR binding to negatively feedback on Ca2+ binding itself and/or its effect on the gating ring. The precise location of CLR binding to cbv1 remains speculation. Although we did identify CLR-sensing areas (seven CRAC domains, with CRAC4 including Tyr450) in the slo1 CTD that contribute to CLR-induced cbv1 inhibition (Singh et al., 2012), it remains to be determined whether any of these CRACs actually provide CLR binding or, rather, participates in the CLR sensitivity of cbv1 as an allosteric modulatory site. The role of CRAC domains as CLR binding sites in proteins, including voltage-gated inwardly rectifying K+ channels, has been put into question (Rosenhouse-Dantsker et al., 2013; Rothberg et al., 1996; Bukiya and Dopico, 2017), and we cannot rule out the existence of CLR recognition sites in the cbv1 CTD (e.g., Asn590-Phe1060) rather than the identified CRACs (Bukiya and Dopico, 2017). To complicate structural interpretations further, partial structural elements within different CRACs [or within their reverse sequence; cholesterol consensus domain (CARS), Fantini et al., 2019] with or without involvement of residues outside these motifs, may work individually to conform CLR binding sites of low-affinity, which are usually of regulatory nature (Jian and Levitan, 2022). At any rate, in either of these two scenarios, Ca2+-recognition sites and the putative CLR-recognition sites in the CTD are different (current Fig. 1, and Yuan et al., 2011; Singh et al., 2012; Hite et al., 2017). Thus, activating physiologic Ca2+i, inhibitory CLR, and activatory n-alkanols should all be considered heterotropic ligands of slo1s.

CLR-induced slo1 inhibition, however, differs from that of other BK inhibitors; paxilline effect is more evident at low levels of Ca2+i and at negative transmembrane voltages (Sanchez and McManus, 1996; Zhou and Lingle, 2014). Likewise, lolitrem B inhibits hslo1 currents at Ca2+I < 50 nM (Imlach et al., 2011), which is Ca2+i too low to gate slo1 on its own. Current data show the opposite: CLR-induced inhibition is more robust as Ca2+ reaches activating (≥1 μM) concentrations for slo1. Moreover, at a given Ca2+ level, more depolarizing voltages, which confer higher Po, enable a more robust inhibition of cbv1 by CLR (Figs. 3B–G, 4B–G). Thus, CLR decreases cbv1 Po via a significant interaction with the channel open state(s). This hypothesis finds support from reanalysis of our earlier data documenting that CLR 33 mol% robustly decreases mslo1 Po due to not only a major increase in mean closed time (x16 times) but also a major decrease in mean open time (34% of control) (Crowley et al., 2003). Likewise, 10%–30% w/w CLR decreases the mean open time of rat brain BK channels reconstituted into POPE/POPS (55/45 w/w) to less than 50% of control (Chang et al., 1995).

Considering that CLR inhibition of slo1 involves a major destabilization of the channel open state(s) and increases as Ca2+i activates these channels, it is remarkable that CLR-action begins to attenuate as Ca2+i reaches 100 μM and, furthermore, vanishes in the presence of sub-mM Ca2+i (i.e., 850 μM) (Fig. 4, F–J). Ca2+i at 10–100 μM activates and saturates both high-affinity Ca2+-sensing sites, whereas Ca2+ exceeding ∼1 mM starts occupying the low-affinity site with eventual channel activation (Xia et al., 2002; Bao et al., 2004; Zeng et al., 2005). Thus, the affinity limits of the high-affinity Ca2+-sensing sites match the Ca2+i-dependence of CLR action on wt cbv1 (Fig. 3). As Ca2+ occupancy of the low-affinity sites starts to take place ([Ca2+]free > ∼1 mM), the ability of CLR to decrease Po and i tend to vanish. A simple explanation for the reduction of CLR action as Ca2+i goes from 10 to 850 µM is that Ca2+i binding to the cbv1 low-affinity Ca2+ site increases cbv1 activity and, thus, counteracts the CLR inhibitory action that is coupled to Ca2+i binding to the cbv1 high-affinity Ca2+i sites. However, data demonstrate that (a) CLR does not activate the channel when the two high-affinity Ca2+i sites are nonfunctional (Fig. 6D) and (b) CLR inhibitory action is not amplified in the low-affinity Ca2+i site mutant (Fig. 6, A–C versus Fig. 3, F–J). At Ca2+i>10 μM, however, both recombinant (mslo1) and native BK channels enter a low Po mode, equivalent to a desensitized state(s) (Rothberg et al., 1996; Liu et al., 2008). The lack of CLR action at hundredths of μM Ca2+i thus could reflect the insensitivity of this channel state(s) to CLR. If so, CLR and ethanol actions on slo1 channel gating drastically differ: ethanol inhibition of mslo1 at Ca2+i > 10 μM is driven by favoring channel entry into this Ca2+-desensitized state(s) (Liu et al., 2008).

We should point out that, as observed for data obtained at 850 μM Ca2+, CLR-induced slo1 inhibition was not significant at maximal voltages (>40 mV) when Ca2+ levels were optimal to facilitate CLR action (10–30 µM Ca2+i). At these Ca2+i levels and voltages, cbv1 activity reaches maximal levels (Figs. 3, B–G, 4, B–G). Thus, there seems to be an optimal level of cbv1 activity and/or allosteric coupling between Ca2+i- and voltage-gating that favors CLR-inhibition of cbv1. According to the “lipid-dependent gating” theory, CLR, as a type II lipid, would be expected to disrupt slo1 voltage-sensor activation, as reported for its relative the KvAP channel (Jiang, 2019). The individual parameters that underlie the Ca2+-dependence of CLR on slo1 can be obtained through the Horrigan-Aldrich allosteric gating model (Horrigan and Aldrich, 2002), which requires varied voltage protocols, some of which are not sustainable in a bilayer system. Based on present data, a simplistic model for the CLR-Ca2+i functional interaction on slo1 channels is given in Fig. 8.

Fig. 8.
  • Download figure
  • Open in new tab
  • Download powerpoint
Fig. 8.

Cartoon illustrating the authors’ interpretation of CLR modulation of slo1 channel activity. (A) A lateral view of the channel is shown as a dimer of slo1 subunits, with their relevant domains shown only in one monomer (right): the “core” (conducting and voltage-sensing domains) is shown in blue; the RCK1 domain is in pink; the Ca2+ bowl associated with the RCK2 is in yellow. At lower Ca2+i, the equilibria from low-conducting via intrinsic gating (A) to high conducting states due to Ca2+-sensing by the Ca2+-bowl (B) or by the RCK1 high-affinity site (C) are regulated by CLR leading to decreased Po (black arrow pointing down). The RCK1 high-affinity site sensing of higher [Ca2+i] makes the channel dwell in a low-activity mode (D), this state being largely refractory to CLR modulation, leading to no change in overall Po by this lipid. In addition, CLR does not modify gating involving the RCK1 low-affinity site, which is involved in sensing high µM-mM divalents, whether Ca2+ or Mg2+. (E) Dark blue arrows: outward K+ flow; plus symbols: voltage-sensor; circular “pockets”: RCK1 low-affinity Ca2+ site; triangular notch: high-affinity Ca2+ sites in RCK1 or “bowl”; brown circles: Mg2+; red drops: Ca2+.

The CLR-Ca2+i-slo1 interaction reported here occurs at (a) CLR molar fractions found in native membranes (van Meer et al., 2008; Slayden et al., 2020; Jian and Levitan, 2022) and to modulate BK (Dopico et al., 2012; Bukiya and Dopico, 2019) and (b) at physiologic Ca2+i that gates BK channels: in cerebrovascular myocytes, the activity of sarcoplasmic ryanodine receptors generates local Ca2+ sparks that, given the close vicinity of membrane BK channels and sarcoplasmic ryanodine receptors in these cells, raises Ca2+ to 4–30 µM in the vicinity of the BK channel’s Ca2+-sensors (Pérez et al., 2001). The final effect of CLR on BK channel function, however, is drastically modified by the presence of regulatory β1 subunits, which are highly abundant in cerebrovascular smooth muscle (Kuntamallappanavar and Dopico, 2017; North et al., 2018): CLR enrichment of middle cerebral arteries leads to a trafficking-dependent increase in membrane levels of BK β1, a process that overrides CLR direct inhibition of slo1 channels and leads to an actual increased in BK currents (Bukiya et al., 2021). The gating underpinnings of CLR-β1 antagonism on slo1 channel function remain to be determined, although it is worthy to mention that β1 regulation of slo1 channel activity is significantly linked to Ca2+-driven gating (Meera et al., 1996; Cox and Aldrich, 2000; Orio et al., 2002; Morrow et al., 2006). Present findings, however, make it clear that CLR inhibition of BK channels will be particularly relevant in tissues that poorly express β1, whether naturally or under pathologic conditions. Regarding vascular smooth muscle, it is noteworthy that prevalent pathologies wherein abnormal myogenic tone and vasodilation have been reported, such as arterial hypertension and diabetes, are characterized by a decreased expression of BK β1 (Table I in Dopico et al., 2018) and increased CLR tissue levels.

Acknowledgments

The authors deeply thank Maria Asuncion-Chin and Shivantika Bisen for excellent technical assistance.

Authorship Contributions

Participated in research design: North, Bukiya, Dopico.

Conducted experiments: North, Zhang, Singh, Zaytseva, Slayden.

Contributed new reagents or analytic tools: Bukiya, Dopico.

Performed data analysis: North, Zhang, Singh, Zaytseva, Bukiya.

Wrote or contributed to the writing of the manuscript: North, Zhang, Slayden, Bukiya, Dopico.

Footnotes

    • Received August 13, 2021.
    • Accepted December 27, 2021.
  • This work was supported by National Institutes of Health National Heart, Lung, and Blood Institute [Grant HL147315] (R01 to A.M.D.) and [Grant HL148941] (R01 to A.N.B. and A.M.D.).

  • No author has an actual or perceived conflict of interest with the contents of this article.

  • ↵1 Current affiliation: Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for System Biomedicine, Shanghai Jiao Tong University, Shanghai, China.

  • ↵2 Current affiliation: Department of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston, Texas.

  • ↵3 Current affiliation: Genentech, Oakland, California.

  • ↵4 M.Z., A.K.S., and D.Z. contributed equally to this manuscript.

  • https://doi.org/10.1124/molpharm.121.000392.

  • ↵Embedded ImageThis article has supplemental material available at molpharm.aspetjournals.org.

Abbreviations

BK
calcium- and voltage-gated K+ channel of large conductance
Ca2+
calcium
Ca2+I
calcium present at the cytosolic/cis side of natural membranes/artificial lipid bilayers
CLR
cholesterol
CTD
cytosolic tail domain
I
unitary current amplitude
Mg2+
magnesium; Mg2+ I, magnesium present at the cytosolic/cis side of natural/artificial lipid bilayers
N
number of functional channels present in the bilayer
NPo
index of channel steady-state activity
Po
individual channel opening probability
POPE
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine
POPS
1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-l-serine
RCK
regulator of conductance for K+ domain
  • Copyright © 2022 by The American Society for Pharmacology and Experimental Therapeutics

References

  1. ↵
    1. Addona GH,
    2. Sandermann H Jr.,
    3. Kloczewiak MA, and
    4. Miller KW
    (2003) Low chemical specificity of the nicotinic acetylcholine receptor sterol activation site. Biochim Biophys Acta 1609:177–182.
    OpenUrlPubMed
  2. ↵
    1. Bao L,
    2. Kaldany C,
    3. Holmstrand EC, and
    4. Cox DH
    (2004) Mapping the BKCa channel’s “Ca2+ bowl”: side-chains essential for Ca2+ sensing. J Gen Physiol 123:475–489.
    OpenUrlAbstract/FREE Full Text
  3. ↵
    1. Bao L,
    2. Rapin AM,
    3. Holmstrand EC, and
    4. Cox DH
    (2002) Elimination of the BK(Ca) channel’s high-affinity Ca(2+) sensitivity. J Gen Physiol 120:173–189.
    OpenUrlAbstract/FREE Full Text
  4. ↵
    1. Behrens R,
    2. Nolting A,
    3. Reimann F,
    4. Schwarz M,
    5. Waldschütz R, and
    6. Pongs O
    (2000) hKCNMB3 and hKCNMB4, cloning and characterization of two members of the large-conductance calcium-activated potassium channel β subunit family. FEBS Lett 474:99–106.
    OpenUrlCrossRefPubMed
  5. ↵
    1. Brenner R,
    2. Peréz GJ,
    3. Bonev AD,
    4. Eckman DM,
    5. Kosek JC,
    6. Wiler SW,
    7. Patterson AJ,
    8. Nelson MT, and
    9. Aldrich RW
    (2000) Vasoregulation by the β1 subunit of the calcium-activated potassium channel. Nature 407:870–876.
    OpenUrlCrossRefPubMed
  6. ↵
    1. Bukiya AN,
    2. Belani JD,
    3. Rychnovsky S, and
    4. Dopico AM
    (2011b) Specificity of cholesterol and analogs to modulate BK channels points to direct sterol-channel protein interactions. J Gen Physiol 137:93–110.
    OpenUrlAbstract/FREE Full Text
  7. ↵
    1. Bukiya AN and
    2. Dopico AM
    (2017) Common structural features of cholesterol binding sites in crystallized soluble proteins. J Lipid Res 58:1044–1054.
    OpenUrlAbstract/FREE Full Text
  8. ↵
    1. Bukiya AN and
    2. Dopico AM
    (2019) Regulation of BK channel activity by cholesterol and its derivatives. Adv Exp Med Biol 1115:53–75.
    OpenUrlCrossRef
  9. ↵
    1. Bukiya AN,
    2. Kuntamallappanavar G,
    3. Edwards J,
    4. Singh AK,
    5. Shivakumar B, and
    6. Dopico AM
    (2014a) An alcohol-sensing site in the calcium- and voltage-gated, large conductance potassium (BK) channel. Proc Natl Acad Sci USA 111:9313–9318.
    OpenUrlAbstract/FREE Full Text
  10. ↵
    1. Bukiya AN,
    2. Leo MD,
    3. Jaggar JH, and
    4. Dopico AM
    (2021) Cholesterol activates BK channels by increasing KCNMB1 protein levels in the plasmalemma. J Biol Chem 296:100381.
    OpenUrl
  11. ↵
    1. Bukiya AN,
    2. Liu J,
    3. Toro L, and
    4. Dopico AM
    (2007) Beta1 (KCNMB1) subunits mediate lithocholate activation of large-conductance Ca2+-activated K+ channels and dilation in small, resistance-size arteries. Mol Pharmacol 72:359–369.
    OpenUrlAbstract/FREE Full Text
  12. ↵
    1. Bukiya AN,
    2. McMillan J,
    3. Liu J,
    4. Shivakumar B,
    5. Parrill AL, and
    6. Dopico AM
    (2014b) Activation of calcium- and voltage-gated potassium channels of large conductance by leukotriene B4. J Biol Chem 289:35314–35325.
    OpenUrlAbstract/FREE Full Text
  13. ↵
    1. Bukiya AN,
    2. Vaithianathan T,
    3. Kuntamallappanavar G,
    4. Asuncion-Chin M, and
    5. Dopico AM
    (2011a) Smooth muscle cholesterol enables BK β1 subunit-mediated channel inhibition and subsequent vasoconstriction evoked by alcohol. Arterioscler Thromb Vasc Biol 31:2410–2423.
    OpenUrlAbstract/FREE Full Text
  14. ↵
    1. Bukiya AN,
    2. Vaithianathan T,
    3. Toro L, and
    4. Dopico AM
    (2008) The second transmembrane domain of the large conductance, voltage- and calcium-gated potassium channel β(1) subunit is a lithocholate sensor. FEBS Lett 582:673–678.
    OpenUrlCrossRefPubMed
  15. ↵
    1. Bukiya AN,
    2. Vaithianathan T,
    3. Toro L, and
    4. Dopico AM
    (2009) Channel beta2–4 subunits fail to substitute for beta1 in sensitizing BK channels to lithocholate. Biochem Biophys Res Commun 390:995–1000.
    OpenUrlCrossRefPubMed
  16. ↵
    1. Chang HM,
    2. Reitstetter R,
    3. Mason RP, and
    4. Gruener R
    (1995) Attenuation of channel kinetics and conductance by cholesterol: an interpretation using structural stress as a unifying concept. J Membr Biol 143:51–63.
    OpenUrlPubMed
  17. ↵
    1. Cox DH and
    2. Aldrich RW
    (2000) Role of the beta1 subunit in large-conductance Ca(2+)-activated K(+) channel gating energetics. Mechanisms of enhanced Ca(2+) sensitivity. J Gen Physiol 116:411–432.
    OpenUrlAbstract/FREE Full Text
  18. ↵
    1. Crowley JJ,
    2. Treistman SN, and
    3. Dopico AM
    (2003) Cholesterol antagonizes ethanol potentiation of human brain BKCa channels reconstituted into phospholipid bilayers. Mol Pharmacol 64:365–372.
    OpenUrlAbstract/FREE Full Text
  19. ↵
    1. Cui J,
    2. Cox DH, and
    3. Aldrich RW
    (1997) Intrinsic voltage dependence and Ca2+ regulation of mslo large conductance Ca-activated K+ channels. J Gen Physiol 109:647–673.
    OpenUrlAbstract/FREE Full Text
  20. ↵
    1. Dopico AM
    (2003) Ethanol sensitivity of BK(Ca) channels from arterial smooth muscle does not require the presence of the beta 1-subunit. Am J Physiol Cell Physiol 284:C1468–C1480.
    OpenUrlCrossRefPubMed
  21. ↵
    1. Dopico AM,
    2. Bukiya AN, and
    3. Jaggar JH
    (2018) Calcium- and voltage-gated BK channels in vascular smooth muscle. Pflugers Arch 470:1271–1289.
    OpenUrl
  22. ↵
    1. Dopico AM,
    2. Bukiya AN, and
    3. Singh AK
    (2012) Large conductance, calcium- and voltage-gated potassium (BK) channels: regulation by cholesterol. Pharmacol Ther 135:133–150.
    OpenUrlCrossRefPubMed
  23. ↵
    1. Fantini J,
    2. Epand RM, and
    3. Barrantes FJ
    (2019) Cholesterol-recognition motifs in membrane proteins. Adv Exp Med Biol 1135:3–25.
    OpenUrlCrossRef
  24. ↵
    1. Gennis RB
    (1989) Biomembranes: Molecular Structure and Function, Springer-Verlag, New York.
  25. ↵
    1. Gimpl G,
    2. Burger K, and
    3. Fahrenholz F
    (1997) Cholesterol as modulator of receptor function. Biochemistry 36:10959–10974.
    OpenUrlCrossRefPubMed
  26. ↵
    1. Giraldez T and
    2. Rothberg BS
    (2017) Understanding the conformational motions of RCK gating rings. J Gen Physiol 149:431–441 DOI: 10.1085/jgp.201611726.
    OpenUrlAbstract/FREE Full Text
  27. ↵
    1. Goluszko P and
    2. Nowicki B
    (2005) Membrane cholesterol: a crucial molecule affecting interactions of microbial pathogens with mammalian cells. Infect Immun 73:7791–7796.
    OpenUrlFREE Full Text
  28. ↵
    1. Hite RK,
    2. Tao X, and
    3. MacKinnon R
    (2017) Structural basis for gating the high-conductance Ca2+-activated K+ channel. Nature 541:52–57.
    OpenUrlCrossRefPubMed
  29. ↵
    1. Horrigan FT and
    2. Aldrich RW
    (1999) Allosteric voltage gating of potassium channels II. Mslo channel gating charge movement in the absence of Ca(2+). J Gen Physiol 114:305–336.
    OpenUrlAbstract/FREE Full Text
  30. ↵
    1. Horrigan FT and
    2. Aldrich RW
    (2002) Coupling between voltage sensor activation, Ca2+ binding and channel opening in large conductance (BK) potassium channels. J Gen Physiol 120:267–305.
    OpenUrlAbstract/FREE Full Text
  31. ↵
    1. Imlach WL,
    2. Finch SC,
    3. Zhang Y,
    4. Dunlop J, and
    5. Dalziel JE
    (2011) Mechanism of action of lolitrem B, a fungal endophyte derived toxin that inhibits BK large conductance Ca2+-activated K+ channels. Toxicon 57:686–694.
    OpenUrlCrossRefPubMed
  32. ↵
    1. Jaggar JH,
    2. Li A,
    3. Parfenova H,
    4. Liu J,
    5. Umstot ES,
    6. Dopico AM, and
    7. Leffler CW
    (2005) Heme is a carbon monoxide receptor for large-conductance Ca2+-activated K+ channels. Circ Res 97:805–812.
    OpenUrlAbstract/FREE Full Text
  33. ↵
    1. Jia Z,
    2. Yazdani M,
    3. Zhang G,
    4. Cui J, and
    5. Chen J
    (2018) Hydrophobic gating in BK channels. Nat Commun 9:3408.
    OpenUrl
  34. ↵
    1. Jian K and
    2. Levitan I
    (2022) Fluorescent imaging to detect cholesterol-protein interactions, in Cholesterol: From Chemistry and Biophysics to the Clinic (Bukiya A and Dopico A, eds) pp 1–616, Academic Press.
  35. ↵
    1. Jiang QX
    (2019) Cholesterol-dependent gating effects on ion channels. Adv Exp Med Biol 1115:167–190.
    OpenUrl
  36. ↵
    1. Kuntamallappanavar G and
    2. Dopico AM
    (2017) BK β1 subunit-dependent facilitation of ethanol inhibition of BK current and cerebral artery constriction is mediated by the β1 transmembrane domain 2. Br J Pharmacol 174:4430–4448.
    OpenUrl
  37. ↵
    1. Kuntamallappanavar G and
    2. Dopico AM
    (2016) Alcohol modulation of BK channel gating depends on β subunit composition. J Gen Physiol 148:419–440.
    OpenUrlAbstract/FREE Full Text
  38. ↵
    1. Levitan I,
    2. Fang Y,
    3. Rosenhouse-Dantsker A, and
    4. Romanenko V
    (2010) Cholesterol and ion channels. Subcell Biochem 51:509–549.
    OpenUrlCrossRefPubMed
  39. ↵
    1. Liu J,
    2. Vaithianathan T,
    3. Manivannan K,
    4. Parrill A, and
    5. Dopico AM
    (2008) Ethanol modulates BKCa channels by acting as an adjuvant of calcium. Mol Pharmacol 74:628–640.
    OpenUrlAbstract/FREE Full Text
  40. ↵
    1. Luu-The V
    (2013) Assessment of steroidogenesis and steroidogenic enzyme functions. J Steroid Biochem Mol Biol 137:176–182.
    OpenUrlCrossRefPubMed
  41. ↵
    1. Meera P,
    2. Wallner M,
    3. Jiang Z, and
    4. Toro L
    (1996) A calcium switch for the functional coupling between alpha (hslo) and beta subunits (KV,Ca beta) of maxi K channels. FEBS Lett 382:84–88.
    OpenUrlCrossRefPubMed
  42. ↵
    1. Morrow JP,
    2. Zakharov SI,
    3. Liu G,
    4. Yang L,
    5. Sok AJ, and
    6. Marx SO
    (2006) Defining the BK channel domains required for beta1-subunit modulation. Proc Natl Acad Sci USA 103:5096–5101.
    OpenUrlAbstract/FREE Full Text
  43. ↵
    1. North K,
    2. Bisen S,
    3. Dopico AM, and
    4. Bukiya AN
    (2018) Tyrosine 450 in the voltage- and calcium-gated potassium channel of large conductance channel pore-forming (slo1) subunit mediates cholesterol protection against alcohol-induced constriction of cerebral arteries. J Pharmacol Exp Ther 367:234–244.
    OpenUrlAbstract/FREE Full Text
  44. ↵
    1. Orio P,
    2. Rojas P,
    3. Ferreira G, and
    4. Latorre R
    (2002) New disguises for an old channel: MaxiK channel β-subunits. News Physiol Sci 17:156–161.
    OpenUrlCrossRefPubMed
  45. ↵
    1. Pérez GJ,
    2. Bonev AD, and
    3. Nelson MT
    (2001) Micromolar Ca(2+) from sparks activates Ca(2+)-sensitive K(+) channels in rat cerebral artery smooth muscle. Am J Physiol Cell Physiol 281:C1769–C1775.
    OpenUrlPubMed
  46. ↵
    1. Pyott SJ,
    2. Meredith AL,
    3. Fodor AA,
    4. Vázquez AE,
    5. Yamoah EN, and
    6. Aldrich RW
    (2007) Cochlear function in mice lacking the BK channel alpha, beta1, or beta4 subunits. J Biol Chem 282:3312–3324.
    OpenUrlAbstract/FREE Full Text
  47. ↵
    1. Ramprasad OG,
    2. Srinivas G,
    3. Rao KS,
    4. Joshi P,
    5. Thiery JP,
    6. Dufour S, and
    7. Pande G
    (2007) Changes in cholesterol levels in the plasma membrane modulate cell signaling and regulate cell adhesion and migration on fibronectin. Cell Motil Cytoskeleton 64:199–216.
    OpenUrlCrossRefPubMed
  48. ↵
    1. Róg T,
    2. Pasenkiewicz-Gierula M,
    3. Vattulainen I, and
    4. Karttunen M
    (2009) Ordering effects of cholesterol and its analogues. Biochim Biophys Acta 1788:97–121.
    OpenUrlCrossRefPubMed
  49. ↵
    1. Rosenhouse-Dantsker A,
    2. Noskov S,
    3. Durdagi S,
    4. Logothetis DE, and
    5. Levitan I
    (2013) Identification of novel cholesterol-binding regions in Kir2 channels. J Biol Chem 288:31154–31164.
    OpenUrlAbstract/FREE Full Text
  50. ↵
    1. Rothberg BS,
    2. Bello RA,
    3. Song L, and
    4. Magleby KL
    (1996) High Ca2+ concentrations induce a low activity mode and reveal Ca2(+)-independent long shut intervals in BK channels from rat muscle. The Journal of Physiology 493:673–689.
    OpenUrlPubMed
  51. ↵
    1. Rothberg BS and
    2. Magleby KL
    (2000) Voltage and Ca2+ activation of single large-conductance Ca2+-activated K+ channels described by a two-tiered allosteric gating mechanism. J Gen Physiol 116:75–99.
    OpenUrlAbstract/FREE Full Text
  52. ↵
    1. Salkoff L,
    2. Butler A,
    3. Ferreira G,
    4. Santi C, and
    5. Wei A
    (2006) High-conductance potassium channels of the SLO family. Nat Rev Neurosci 7:921–931 DOI: 10.1038/nrn1992.
    OpenUrlCrossRefPubMed
  53. ↵
    1. Sanchez M and
    2. McManus OB
    (1996) Paxilline inhibition of the alpha-subunit of the high-conductance calcium-activated potassium channel. Neuropharmacology 35:963–968.
    OpenUrlCrossRefPubMed
  54. ↵
    1. Schroeder HW Jr. and
    2. Cavacini L
    (2010) Structure and function of immunoglobulins. J Allergy Clin Immunol 125 (Suppl 2):S41–S52.
    OpenUrlCrossRefPubMed
  55. ↵
    1. Singh AK,
    2. McMillan J,
    3. Bukiya AN,
    4. Burton B,
    5. Parrill AL, and
    6. Dopico AM
    (2012) Multiple cholesterol recognition/interaction amino acid consensus (CRAC) motifs in cytosolic C tail of Slo1 subunit determine cholesterol sensitivity of Ca2+- and voltage-gated K+ (BK) channels. J Biol Chem 287:20509–20521.
    OpenUrlAbstract/FREE Full Text
  56. ↵
    1. Slayden A,
    2. North K,
    3. Bisen S,
    4. Dopico AM,
    5. Bukiya AN, and
    6. Rosenhouse-Dantsker A
    (2020) Enrichment of mammalian tissues and xenopus oocytes with cholesterol. J Vis Exp 25:157.
  57. ↵
    1. Sooksawate T and
    2. Simmonds MA
    (2001) Influence of membrane cholesterol on modulation of the GABA(A) receptor by neuroactive steroids and other potentiators. Br J Pharmacol 134:1303–1311.
    OpenUrlCrossRefPubMed
  58. ↵
    1. Tao X,
    2. Hite RK, and
    3. MacKinnon R
    (2017) Cryo-EM structure of the open high-conductance Ca2+-activated K+ channel. Nature 541:46–51.
    OpenUrlCrossRefPubMed
  59. ↵
    1. van Meer G,
    2. Voelker DR, and
    3. Feigenson GW
    (2008) Membrane lipids: where they are and how they behave. Nat Rev Mol Cell Biol 9:112–124.
    OpenUrlCrossRefPubMed
  60. ↵
    1. Wang L and
    2. Sigworth FJ
    (2009) Structure of the BK potassium channel in a lipid membrane from electron cryomicroscopy. Nature 461:292–295 DOI: 10.1038/nature08291.
    OpenUrlCrossRefPubMed
  61. ↵
    1. Whitt JP,
    2. Montgomery JR, and
    3. Meredith AL
    (2016) BK channel inactivation gates daytime excitability in the circadian clock. Nat Commun 7:10837.
    OpenUrlCrossRefPubMed
  62. ↵
    1. Wu Y,
    2. Yang Y,
    3. Ye S, and
    4. Jiang Y
    (2010) Structure of the gating ring from the human large-conductance Ca(2+)-gated K(+) channel. Nature 466:393–397 DOI: 10.1038/nature09252.
    OpenUrlCrossRefPubMed
  63. ↵
    1. Xia XM,
    2. Zeng X, and
    3. Lingle CJ
    (2002) Multiple regulatory sites in large-conductance calcium-activated potassium channels. Nature 418:880–884 DOI: 10.1038/nature00956.
    OpenUrlCrossRefPubMed
  64. ↵
    1. Yan J and
    2. Aldrich RW
    (2010) LRRC26 auxiliary protein allows BK channel activation at resting voltage without calcium. Nature 466:513–516.
    OpenUrlCrossRefPubMed
  65. ↵
    1. Yan J and
    2. Aldrich RW
    (2012) BK potassium channel modulation by leucine-rich repeat-containing proteins. Proc Natl Acad Sci USA 109:7917–7922.
    OpenUrlAbstract/FREE Full Text
  66. ↵
    1. Yuan C,
    2. Chen M,
    3. Covey DF,
    4. Johnston LJ, and
    5. Treistman SN
    (2011) Cholesterol tuning of BK ethanol response is enantioselective, and is a function of accompanying lipids. PLoS One 6:e27572.
    OpenUrlCrossRefPubMed
  67. ↵
    1. Yuan C,
    2. O’Connell RJ,
    3. Jacob RF,
    4. Mason RP, and
    5. Treistman SN
    (2007) Regulation of the gating of BKCa channel by lipid bilayer thickness. J Biol Chem 282:7276–7286.
    OpenUrlAbstract/FREE Full Text
  68. ↵
    1. Yuan P,
    2. Leonetti MD,
    3. Pico AR,
    4. Hsiung Y, and
    5. MacKinnon R
    (2010) Structure of the human BK channel Ca2+-activation apparatus at 3.0 A resolution. Science 329:182–186.
    OpenUrlAbstract/FREE Full Text
  69. ↵
    1. Zeng XH,
    2. Xia XM, and
    3. Lingle CJ
    (2005) Divalent cation sensitivity of BK channel activation supports the existence of three distinct binding sites. J Gen Physiol 125:273–286.
    OpenUrlAbstract/FREE Full Text
  70. ↵
    1. Zhang X,
    2. Solaro CR, and
    3. Lingle CJ
    (2001) Allosteric regulation of BK channel gating by Ca(2+) and Mg(2+) through a nonselective, low affinity divalent cation site. J Gen Physiol 118:607–636.
    OpenUrlAbstract/FREE Full Text
  71. ↵
    1. Zhou Y and
    2. Lingle CJ
    (2014) Paxilline inhibits BK channels by an almost exclusively closed-channel block mechanism. J Gen Physiol 144:415–440.
    OpenUrlAbstract/FREE Full Text
PreviousNext
Back to top

In this issue

Molecular Pharmacology: 101 (3)
Molecular Pharmacology
Vol. 101, Issue 3
1 Mar 2022
  • Table of Contents
  • Table of Contents (PDF)
  • About the Cover
  • Index by author
  • Editorial Board (PDF)
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Molecular Pharmacology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Cholesterol Inhibition of Slo1 Channels Is Calcium-Dependent and Can Be Mediated by Either High-Affinity Calcium-Sensing Site in the Slo1 Cytosolic Tail
(Your Name) has forwarded a page to you from Molecular Pharmacology
(Your Name) thought you would be interested in this article in Molecular Pharmacology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleArticle

Cholesterol-Calcium Interaction on BK Channels

Kelsey C. North, Man Zhang, Aditya K. Singh, Dasha Zaytseva, Alexandria V. Slayden, Anna N. Bukiya and Alex M. Dopico
Molecular Pharmacology March 1, 2022, 101 (3) 132-143; DOI: https://doi.org/10.1124/molpharm.121.000392

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Research ArticleArticle

Cholesterol-Calcium Interaction on BK Channels

Kelsey C. North, Man Zhang, Aditya K. Singh, Dasha Zaytseva, Alexandria V. Slayden, Anna N. Bukiya and Alex M. Dopico
Molecular Pharmacology March 1, 2022, 101 (3) 132-143; DOI: https://doi.org/10.1124/molpharm.121.000392
Reddit logo Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Introduction
    • Materials and Methods
    • Results
    • Discussion
    • Acknowledgments
    • Authorship Contributions
    • Footnotes
    • Abbreviations
    • References
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF + SI
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Cysteine151 in Keap1 Drives CDDO-Me Pharmacodynamic Action
  • Allosteric Modulation of Metabotropic Glutamate Receptor 1
  • Mechanism of Selective Action of Paraherquamide A
Show more Articles

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About Molecular Pharmacology
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Journal of Pharmacology and Experimental Therapeutics
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0111 (Online)

Copyright © 2023 by the American Society for Pharmacology and Experimental Therapeutics