Abstract
The ability of isoproterenol, epinephrine, norepinephrine, and salbutamol to desensitize the beta receptor-coupled cyclic 3',5'-AMP response of human diploid fibroblasts in tissue culture correlates well with their activity as beta stimulants. Partial desensitization of the cells results in a marked fall in the maximal cyclic AMP response to isoproterenol: fully desensitized cells are totally unresponsive to isoproterenol. Recovery of beta adrenergic sensitivity is a slow process and is delayed by extremely low concentrations of isoproterenol (picomolar). Recovery is also inhibited by concentrations of puromycin, cycloheximide, and actinomycin D that inhibit protein or RNA synthesis.
- Copyright ©, 1975, by Academic Press, Inc.
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