Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Molecular Pharmacology
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Molecular Pharmacology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit molpharm on Facebook
  • Follow molpharm on Twitter
  • Follow molpharm on LinkedIn
Research ArticleArticle

Adenosine Cyclic 3',5'-Monophosphate in the Liver Fluke, Fasciola hepatica

II. Activation of Protein Kinase by 5-Hydroxytryptamine

SUSAN GENTLEMAN, SANDERS L. ABRAHAMS and TAG E. MANSOUR
Molecular Pharmacology January 1976, 12 (1) 59-68;
SUSAN GENTLEMAN
Department of Pharmacology, Stanford University School of Medicine, Stanford, California 94305
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
SANDERS L. ABRAHAMS
Department of Pharmacology, Stanford University School of Medicine, Stanford, California 94305
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
TAG E. MANSOUR
Department of Pharmacology, Stanford University School of Medicine, Stanford, California 94305
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

Adenosine cyclic 3',5'-monophosphate (cAMP)-dependent protein kinase activity has been found in both the 27,000 x g particulate and supernatant fractions of homogenates from the liver fluke, Fasciola hepatica. The enzyme activity was 2-5 times greater in the anterior end ("head") of the fluke than in the posterior end. Half-maximal activation of the protein kinase in both fractions was obtained at 0.1-0.4 µM cAMP. When the enzyme was assayed in the presence of cyclic nucleotide, the apparent Km for protamine was 0.1 mg/ml and the apparent Km for MgATP was 20-50 µM in the two homogenate fractions. Incubation of fluke heads for 5 min with 1 mM 5-hydroxytryptamine resulted in an increase in protein kinase activity of both homogenate fractions when assayed in the absence of added cAMP. The protein kinase activity assayed in the presence of 5 µM cAMP was increased in the particulate fraction and decreased in the supernatant fraction. The degree of activation of the enzyme by the cyclic nucleotide was markedly reduced in both homogenate fractions from heads that had been incubated with 5-hydroxytryptamine. The time course of accumulation of endogenous cAMP correlated with activation of protein kinase, except during the first minute of incubation with 5-hydroxytryptamine. The degree of activation of protein kinase by added cAMP was inversely correlated with endogenous cyclic nucleotide during incubation with 5-hydroxytryptamine. Incubation of fluke heads with both 5-hydroxytryptamine and D-lysergic acid diethylamide together reduced the activation of protein kinase by 5-hydroxytryptamine. Incubation of fluke heads with only D-lysergic acid diethylamide had no effect on protein kinase activity, cAMP increased phosphorylation of fluke protein in both fractions of the homogenate. This phosphorylation was less sensitive to activation by the cyclic nucleotide when the fluke heads had been incubated with 5-hydroxytryptamine. Thus 5-hydroxytryptamine can stimulate protein kinase activity toward endogenous proteins. The results indicate that the physiological effects of 5-hydroxytryptamine are mediated by cAMP activation of protein kinase.

  • Copyright © 1976 by Academic Press, Inc.

MolPharm articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Molecular Pharmacology
Vol. 12, Issue 1
1 Jan 1976
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Back Matter (PDF)
  • Editorial Board (PDF)
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Molecular Pharmacology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Adenosine Cyclic 3',5'-Monophosphate in the Liver Fluke, Fasciola hepatica
(Your Name) has forwarded a page to you from Molecular Pharmacology
(Your Name) thought you would be interested in this article in Molecular Pharmacology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleArticle

Adenosine Cyclic 3',5'-Monophosphate in the Liver Fluke, Fasciola hepatica

SUSAN GENTLEMAN, SANDERS L. ABRAHAMS and TAG E. MANSOUR
Molecular Pharmacology January 1, 1976, 12 (1) 59-68;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Research ArticleArticle

Adenosine Cyclic 3',5'-Monophosphate in the Liver Fluke, Fasciola hepatica

SUSAN GENTLEMAN, SANDERS L. ABRAHAMS and TAG E. MANSOUR
Molecular Pharmacology January 1, 1976, 12 (1) 59-68;
Reddit logo Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Antimicrobial and Antileukemic Transportan 10 Conjugates
  • Pharmacological characterization of zebrafish H1 receptor
  • Bhave and Forman
Show more Article

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About Molecular Pharmacology
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Journal of Pharmacology and Experimental Therapeutics
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0111 (Online)

Copyright © 2023 by the American Society for Pharmacology and Experimental Therapeutics