Abstract
The purpose of this study was to determine whether mammalian cells can utilize 2-fluoro-L-histidine in protein synthesis. Rat pineal glands were incubated in organ culture in medium containing 2-fluoro-L-[4-3]histidine (3 mM); trichloracetic acid-insoluble material from these glands contained radioactivity. Incorporation of radioactivity was decreased in the presence of histidine (3 mM). After the 3H-labeled macromolecules were treated with a protease, over 75% of the radioactivity was soluble in trichloracetic acid. Amino acid analysis of the trichloracetic acid-soluble material indicated that the majority of the liberated radioactivity was 2-fluoro-L-[3H]histidine. These findings demonstrate that mammalian cells can incorporate 2-fluoro-L-histidine into newly synthesized proteins. In view of this, it would appear likely that the reported inhibitory effects of 2-fluoro-L-histidine on enzyme induction could result, in part, from the incorporation of the analogue into proteins.
- Copyright © 1977 by Academic Press, Inc.
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