Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Molecular Pharmacology
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Molecular Pharmacology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit molpharm on Facebook
  • Follow molpharm on Twitter
  • Follow molpharm on LinkedIn
Research ArticleArticle

Glucocorticoids Selectively Decrease the Synthesis of Hydroxylated Collagen Peptides

ROBERT A. NEWMAN and KENNETH R. CUTRONEO
Molecular Pharmacology January 1978, 14 (1) 185-198;
ROBERT A. NEWMAN
Department of Biochemistry, University of Vermont College of Medicine, Burlington, Vermont 05401
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
KENNETH R. CUTRONEO
Department of Biochemistry, University of Vermont College of Medicine, Burlington, Vermont 05401
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

Multiple daily injections of triamcinolone diacetate to newborn rats result in decreased body and skin weight gain, which is related to a specific decrease of collagen polypeptide synthesis. The effects of glucocorticoid administration on protein synthesis of the dermis was determined by measurement of the incorporation of labeled proline. The percentage decrease in collagen synthesis in triamcinolone-treated animals was greater than that of non-collagen protein synthesis at all doses examined, indicating a selective effect of glucocorticoids on collagen synthesis. This selective decrease of collagen synthesis was greatest after multiple injections at higher doses of the steroid. DNA synthesis was also suppressed after multiple injections of steroid. Steroid administration resulted in a decrease in prolyl hydroxylase activity (EC 1.14.11.2) in a dose- and time-dependent manner. Lysyl hydroxylase activity (EC 1.14.11.4) was decreased to the same extent as prolyl hydroxylase activity, while glucose 6-phosphate dehydrogenase activity (EC 1.1.1.49) was unchanged and tyrosine aminotransferase activity (EC 2.6.1.5) was slightly elevated. Collagenase digestion of nascent polypeptide chains released from dermal polysomes demonstrated a selective, dose-dependent decrease in collagen polypeptide synthesis. Furthermore, collagen nascent chain synthesis was decreased to the same extent as hydroxyproline formation, indicating that glucocorticoids do not cause the synthesis and subsequent accumulation of underhydroxylated collagen.

ACKNOWLEDGMENTS The authors wish to thank Dr. Brian Little for his consultation in evaluating the histological sections, and Dr. Cassius Bordolon for his consultation on preparing the wheat germ lysate system. We also acknowledge the expert technical assistance of Ms. Elizabeth Tuthill, Ms. Gloria Hoff, Mr. Brian McNelis, and Mr. James Evans.

  • Copyright © 1978 by Academic Press, Inc.

MolPharm articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Molecular Pharmacology
Vol. 14, Issue 1
1 Jan 1978
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Back Matter (PDF)
  • Editorial Board (PDF)
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Molecular Pharmacology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Glucocorticoids Selectively Decrease the Synthesis of Hydroxylated Collagen Peptides
(Your Name) has forwarded a page to you from Molecular Pharmacology
(Your Name) thought you would be interested in this article in Molecular Pharmacology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleArticle

Glucocorticoids Selectively Decrease the Synthesis of Hydroxylated Collagen Peptides

ROBERT A. NEWMAN and KENNETH R. CUTRONEO
Molecular Pharmacology January 1, 1978, 14 (1) 185-198;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Research ArticleArticle

Glucocorticoids Selectively Decrease the Synthesis of Hydroxylated Collagen Peptides

ROBERT A. NEWMAN and KENNETH R. CUTRONEO
Molecular Pharmacology January 1, 1978, 14 (1) 185-198;
del.icio.us logo Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Michaelis Menten quantification of GPCR-G protein signalling
  • Human mAb 3F1 targeting the fuctional epitopes of Siglec-15
  • The regulation and mechanisms of ImKTX58 on KV1.3 channel
Show more Article

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About Molecular Pharmacology
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Journal of Pharmacology and Experimental Therapeutics
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0111 (Online)

Copyright © 2022 by the American Society for Pharmacology and Experimental Therapeutics