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Molecular Pharmacology

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Research ArticleArticle

Modulation of Intracellular Adenosine Cyclic 3',5'-Monophosphate and Contractility of Rat Uterus by Prostaglandins and Polyunsaturated Fatty Acids

MARIE-FRANÇOISE VESIN, LIEN DO KHAC and SIMONE HARBON
Molecular Pharmacology January 1978, 14 (1) 24-37;
MARIE-FRANÇOISE VESIN
Institut de Biochimie, Université Paris-Sud, 91405 Orsay Cedex, France
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LIEN DO KHAC
Institut de Biochimie, Université Paris-Sud, 91405 Orsay Cedex, France
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SIMONE HARBON
Institut de Biochimie, Université Paris-Sud, 91405 Orsay Cedex, France
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Abstract

Prostaglandins E1, (PGE1) and E2 stimulated contractions of estrogen-treated rat uteri. They also caused the accumulation of adenosine cyclic 3',5'-monophosphate (cAMP) in the isolated myometrium and endometrium. Both stimulations were also induced by arachidonic (C20:4) and homo-γ-linoleic (C20:3) acids, which are direct precursors of prostaglandins. Other unsaturated fatty acids, including oleic, linoleic, γ-linolenic, and eicosadienoic, were without effect. The stimulations of contraction and adenylate cyclase activity by arachidonic and homo-γ-linolenic acids were very rapid, detectable at 30 sec, dose-dependent, and abolished by the specific cyclooxygenase inhibitors indomethacin, meclofenamic acid, and eicosatetraynoic acid. Hence the conversion of C20:3 and C20:4 fatty acids to prostaglandin or prostaglandin-like material occurred in both myometrium and endometrium and was responsible for the observed activations. The levels of cAMP can therefore be modulated by local prostaglandin effectors in a manner similar to that produced by exogenous PGE compounds. The stimulatory effects of arachidonic acid were followed by an unresponsive phase, as shown by the subsequent inhibited responses of the myometrium to contractile agents such as prostaglandins and carbamylcholine as well as by the suppression of adenylate cyclase activation by either epinephrine or PGE2, in both endometrium and myometrium. The latter inhibition was not readily reversible. It did not involve the prostaglandin synthetase system, inasmuch as indomethacin failed to prevent the subsequent inhibitory effect of arachidonic acid. Furthermore, similar marked inhibition was exerted by linoleic, γ-linolenic, and eicosatetraynoic acids. These results suggest the possible alteration by fatty acids of a membrane architecture crucial for adenylate cyclase activation and signal transmission during hormone-induced contraction.

ACKNOWLEDGMENTS We are indebted to Professor H. Clauser for his support and helpful discussions. We wish to thank Professor V . Najjar for valuable comments and critically reviewing the manuscript. A special acknowledgment is extended to Mrs. Robichon for technical assistance and Mrs. Crosnier for typing the manuscript.

  • Copyright © 1978 by Academic Press, Inc.

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Molecular Pharmacology
Vol. 14, Issue 1
1 Jan 1978
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Research ArticleArticle

Modulation of Intracellular Adenosine Cyclic 3',5'-Monophosphate and Contractility of Rat Uterus by Prostaglandins and Polyunsaturated Fatty Acids

MARIE-FRANÇOISE VESIN, LIEN DO KHAC and SIMONE HARBON
Molecular Pharmacology January 1, 1978, 14 (1) 24-37;

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Research ArticleArticle

Modulation of Intracellular Adenosine Cyclic 3',5'-Monophosphate and Contractility of Rat Uterus by Prostaglandins and Polyunsaturated Fatty Acids

MARIE-FRANÇOISE VESIN, LIEN DO KHAC and SIMONE HARBON
Molecular Pharmacology January 1, 1978, 14 (1) 24-37;
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