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Molecular Pharmacology

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Research ArticleArticle

Mechanism of Action of Amantadine on Neuromuscular Transmission

MING-CHENG TSAI, NABIL A. MANSOUR, AMIRA T. ELDEFRAWI, MOHYEE E. ELDEFRAWI and EDSON X. ALBUQUERQUE
Molecular Pharmacology September 1978, 14 (5) 787-803;
MING-CHENG TSAI
Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, Baltimore, Maryland 21201
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NABIL A. MANSOUR
Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, Baltimore, Maryland 21201
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AMIRA T. ELDEFRAWI
Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, Baltimore, Maryland 21201
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MOHYEE E. ELDEFRAWI
Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, Baltimore, Maryland 21201
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EDSON X. ALBUQUERQUE
Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, Baltimore, Maryland 21201
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This article has a correction. Please see:

  • Erratum for Volume 14, No. 5, September 1978 - May 01, 1979

Abstract

The effect of amantadine on neuromuscular transmission was studied in frog sartorius and rat soleus and diaphragm muscle preparations. Amantadine blocked transmission reversibly, while having negligible presynaptic effects and inducing 10-20 mV depolarization of the muscle membrane that was tetrodotoxin insensitive. Its most pronounced effect was on the acetylcholine receptor-mediated postsynaptic conductances. Amantadine caused voltage-dependent attenuation of the peak endplate current amplitude and marked departure from linearity in the current voltage relationship. The drug altered the voltage-dependence of the falling phase of the endplate current, and reduced the slope of the relationship between endplate current half decay time and membrane potential with subsequent reversal of the slope such that the endplate current became faster with hyperpolarization. In addition, amantadine was found to inhibit the carbamylcholine-induced 22Na efflux from microsacs formed from Torpedo electric organ membranes, suggesting that amantadine had postsynaptic action. Amantadine did not inhibit binding of [3H]acetylcholine or [125I]α-bungarotoxin to the acetylcholine receptor but inhibited competitively the binding of [3H]perhydrohistrionicotoxin to the ionic channel of the acetylcholine receptor with Ki of 60 µM. These effects of amantadine on postsynaptic ionic current, coupled with its inability to protect against blockade of transmission by α-bungarotoxin and its inhibition of [3H]perhydrohistrionicotoxin binding suggest that amantadine blocks neuromuscular transmission by reacting with the ionic channel of the acetylcholine receptor.

ACKNOWLEDGMENT We are grateful to Drs. B. Witkop and J. Daly of the National Institutes of Health for kindly providing us with [3H]H12-HTX, HTX and H8-HTX, and Drs. V. G. Vernier and G. S. Shotzberger of E. I. DuPont de Nemours Co. for donating amantadine. We are most indebted to Ms. Mabel Alice Zelle for the computer analysis and technical assistance during the tenure of this project. We thank David C. Copio for some of the initial studies related to the effect of amantadine on [125I]α-bungarotoxin binding.

  • Copyright © 1978 by Academic Press, Inc.

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Molecular Pharmacology
Vol. 14, Issue 5
1 Sep 1978
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Research ArticleArticle

Mechanism of Action of Amantadine on Neuromuscular Transmission

MING-CHENG TSAI, NABIL A. MANSOUR, AMIRA T. ELDEFRAWI, MOHYEE E. ELDEFRAWI and EDSON X. ALBUQUERQUE
Molecular Pharmacology September 1, 1978, 14 (5) 787-803;

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Research ArticleArticle

Mechanism of Action of Amantadine on Neuromuscular Transmission

MING-CHENG TSAI, NABIL A. MANSOUR, AMIRA T. ELDEFRAWI, MOHYEE E. ELDEFRAWI and EDSON X. ALBUQUERQUE
Molecular Pharmacology September 1, 1978, 14 (5) 787-803;
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