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Molecular Pharmacology

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Research ArticleArticle

Regulation of Synthesis and Degradation of Rat Adrenal Phenylethanolamine N-methyltransferase

III. Stabilization of PNMT Against Thermal and Tryptic Degradation by S-Adenosylmethionine

DAVID M. BERENBEIM, DONA L. WONG, STEVEN J. MASOVER and ROLAND D. CIARANELLO
Molecular Pharmacology September 1979, 16 (2) 482-490;
DAVID M. BERENBEIM
Child Psychiatry Program, Department of Psychiatry and Behavioral Sciences, Stanford University Medical Center, Stanford, California 94305
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DONA L. WONG
Child Psychiatry Program, Department of Psychiatry and Behavioral Sciences, Stanford University Medical Center, Stanford, California 94305
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STEVEN J. MASOVER
Child Psychiatry Program, Department of Psychiatry and Behavioral Sciences, Stanford University Medical Center, Stanford, California 94305
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ROLAND D. CIARANELLO
Child Psychiatry Program, Department of Psychiatry and Behavioral Sciences, Stanford University Medical Center, Stanford, California 94305
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Abstract

The regulation of phenylethanolamine N-methyltransferase degradation in vivo is mediated via adrenal glucocorticoids. The glucocorticoid inhibition of PNMT degradation is mediated via an endogenous adrenal stabilizing factor whose levels are controlled by glucocorticoids. A partially purified preparation of the endogenous stabilizing factor has an absorption maximum of 265 nm and migrates with S-adenosylmethionine and 3',5'-cyclic AMP on paper chromatography. S-adenosylmethionine is highly effective in stabilizing PNMT against both thermal and tryptic degradation, while cyclic AMP is ineffective as a stabilizing agent. S-adenosylhomocysteine, the demethylated product formed from S-adenosylmethionine during transmethylation stabilizes PNMT against tryptic but not thermal degradation. S-adenosylmethionine administration to hypophy-sectomized rats partially restores PNMT levels in vivo and enzyme thermal stability in vitro.

ACKNOWLEDGMENTS The authors wish to express their gratitude to Dr. Helena Kramer of the Department of Psychiatry and Behavioral Sciences for her invaluable help in the statistical analysis of the regression data.

  • Copyright © 1979 by Academic Press, Inc.

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Molecular Pharmacology
Vol. 16, Issue 2
1 Sep 1979
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Research ArticleArticle

Regulation of Synthesis and Degradation of Rat Adrenal Phenylethanolamine N-methyltransferase

DAVID M. BERENBEIM, DONA L. WONG, STEVEN J. MASOVER and ROLAND D. CIARANELLO
Molecular Pharmacology September 1, 1979, 16 (2) 482-490;

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Research ArticleArticle

Regulation of Synthesis and Degradation of Rat Adrenal Phenylethanolamine N-methyltransferase

DAVID M. BERENBEIM, DONA L. WONG, STEVEN J. MASOVER and ROLAND D. CIARANELLO
Molecular Pharmacology September 1, 1979, 16 (2) 482-490;
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