Abstract
This paper contrasts the inhibition of deoxyuridine incorporation by the lipid-soluble antifolate, DDMP, and methotrexate in the human lymphoblastoid cell line, WIL-2. It shows how deoxyuridine incorporation can be used as an indirect tool for assay of drug uptake and facilitates interpretation of experiments with labeled drugs. Whereas 1 µM methotrexate takes 15-30 minutes to maximally inhibit deoxyuridine incorporation, DDMP maximally inhibits incorporation within five seconds of addition to the cell culture. These protocols expand the potential of deoxyuridine-based assays in analysis of antifolate action and may be useful for in vitro testing of potential tumor responses to antifolates.
- Copyright © 1979 by Academic Press, Inc.
MolPharm articles become freely available 12 months after publication, and remain freely available for 5 years.Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page.
|
Log in using your username and password
Purchase access
You may purchase access to this article. This will require you to create an account if you don't already have one.