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Research ArticleArticle

Poly (L-Lysine) and Poly (D-Lysine) Conjugates of Methotrexate: Different Inhibitory Effect on Drug Resistant Cells

WEI-CHIANG SHEN and HUGUES J.-P. RYSER
Molecular Pharmacology September 1979, 16 (2) 614-622;
WEI-CHIANG SHEN
Department of Pathology and Pharmacology, Boston University School of Medicine, Boston, MA 02118
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HUGUES J.-P. RYSER
Department of Pathology and Pharmacology, Boston University School of Medicine, Boston, MA 02118
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Abstract

Conjugation of methotrexate to poly(L-lysine) markedly increases its cellular uptake and offers a new way to overcome drug resistance related to deficient transport. Conjugates using poly(L-lysines) of molecular weights varying from 3,100 to 130,000 have comparable effects on a drug resistant CHO cell line. Conjugates using poly(D-lysine), however, have no effect on either resistant or normal CHO cells. Both the L- and D-isomeric conjugates are taken up by cells in comparable fashion. Since even the biologically active conjugates are poor inhibitors of dihydrofolate reductase (5,6,7,8-tetrahydrofolate:NADP+ oxidoreductase; EC 1.5.1.3) in vitro, it is concluded that the conjugate must give rise in the cell to a pharmacologically active breakdown product. Such a product can be detected in cells exposed to the L-isomeric, but not in cells exposed to the D-isomeric conjugates. Excess of poly(L-lysine) only moderately decreases methotrexate-poly(L-lysine) uptake but markedly decreases its inhibitory effect on cell growth.

ACKNOWLEDGMENTS We thank Dr. Wayne F. Flintoff, of the University of Western Ontario, for making available to us the methotrexate-resistant CHO cell lines; Dr. Richard A. Laursen for the amino acid analysis of the MTX-poly(L-lys) conjugates; and Ms. Lucie van Heeckeren for technical assistance.

  • Copyright © 1979 by Academic Press, Inc.

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Molecular Pharmacology
Vol. 16, Issue 2
1 Sep 1979
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Research ArticleArticle

Poly (L-Lysine) and Poly (D-Lysine) Conjugates of Methotrexate: Different Inhibitory Effect on Drug Resistant Cells

WEI-CHIANG SHEN and HUGUES J.-P. RYSER
Molecular Pharmacology September 1, 1979, 16 (2) 614-622;

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Research ArticleArticle

Poly (L-Lysine) and Poly (D-Lysine) Conjugates of Methotrexate: Different Inhibitory Effect on Drug Resistant Cells

WEI-CHIANG SHEN and HUGUES J.-P. RYSER
Molecular Pharmacology September 1, 1979, 16 (2) 614-622;
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