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Molecular Pharmacology

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Research ArticleArticle

Opiate-Like Materials in the Adrenal Medulla: Evidence for Storage and Secretion with Catecholamines

O. HUMBERTO VIVEROS, EMANUEL J. DILIBERTO JR., ELI HAZUM and KWEN-JEN CHANG
Molecular Pharmacology November 1979, 16 (3) 1101-1108;
O. HUMBERTO VIVEROS
Department of Medicinal Biochemistry and Department of Molecular Biology, Wellcome Research Laboratories, Research Triangle Park, N. C. 27709
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EMANUEL J. DILIBERTO JR.
Department of Medicinal Biochemistry and Department of Molecular Biology, Wellcome Research Laboratories, Research Triangle Park, N. C. 27709
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ELI HAZUM
Department of Medicinal Biochemistry and Department of Molecular Biology, Wellcome Research Laboratories, Research Triangle Park, N. C. 27709
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KWEN-JEN CHANG
Department of Medicinal Biochemistry and Department of Molecular Biology, Wellcome Research Laboratories, Research Triangle Park, N. C. 27709
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Abstract

Opiate-like materials, as measured by the radioreceptor assay, were found in the adrenal gland of 7 mammalian species including man. The opiate-like materials are confined to the adrenal medulla, and followed a pattern identical to catecholamines on differential and isopycnic centrifugation. In the isolated perfused dog adrenal gland, acetylcholine stimulates a Ca2+-dependent secretion of opiate-like materials and catecholamines in the same molar ratio as they are stored within the chromaffin cell. Ba2+ also stimulates the secretion of both products from the adrenal gland. Separation of the opiate-like materials by Sephadex G-50 and reverse phase high pressure liquid chromatography indicates the presence of four peaks of opiate-like materials of molecular weights below 2,000. Two of these peaks comigrate in these systems with authentic met- and leu-enkephalins and react with the corresponding antibodies. These results indicate that enkephalins and other low molecular weight opiate-like materials are stored in and secreted from the chromaffin vesicles with catecholamines in the adrenal glands. These findings support a role for enkephalins as neurotransmitters and/or neurohormones. Also, they suggest that enkephalins and other opiate-like materials may exert important neuroendocrine functions outside the central nervous system.

ACKNOWLEDGMENTS The technical assistance of Mrs. Martha Abou-Donia, Ms. Pamela Allen and Mr. Mark Collins is gratefully acknowledged. We are grateful to Drs. Pedro Cuatrecasas and Charles A. Nichol for reviewing the manuscript, helpful discussions and constant encouragement. Human adrenal glands were obtained through the collaboration of Dr. Philip C. Pratt and his staff at the Veterans Administration Hospital, Durham, N. C.

  • Copyright © 1979 by Academic Press, Inc.

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Molecular Pharmacology
Vol. 16, Issue 3
1 Nov 1979
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Research ArticleArticle

Opiate-Like Materials in the Adrenal Medulla: Evidence for Storage and Secretion with Catecholamines

O. HUMBERTO VIVEROS, EMANUEL J. DILIBERTO, ELI HAZUM and KWEN-JEN CHANG
Molecular Pharmacology November 1, 1979, 16 (3) 1101-1108;

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Research ArticleArticle

Opiate-Like Materials in the Adrenal Medulla: Evidence for Storage and Secretion with Catecholamines

O. HUMBERTO VIVEROS, EMANUEL J. DILIBERTO, ELI HAZUM and KWEN-JEN CHANG
Molecular Pharmacology November 1, 1979, 16 (3) 1101-1108;
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