Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Molecular Pharmacology
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Molecular Pharmacology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit molpharm on Facebook
  • Follow molpharm on Twitter
  • Follow molpharm on LinkedIn
Research ArticleArticle

Induction of UDP-Glucuronosyltransferase Activity in the Reuber H-4-II-E Hepatoma Cell Culture

NAJMA MALIK, GLENN M. KOTEEN and IDA S. OWENS
Molecular Pharmacology November 1979, 16 (3) 950-960;
NAJMA MALIK
Developmental Pharmacology Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20205
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
GLENN M. KOTEEN
Developmental Pharmacology Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20205
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
IDA S. OWENS
Developmental Pharmacology Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20205
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

The rat Reuber H-4-II-E continuous cell line contains 4-methylumbelliferone-UDP-glucuronosyltransferase activity. This enzyme is induced 3- to 6-fold by polycyclic aromatic compounds such as benzo[a]pyrene, 3-methylcholanthrene, benz[a]anthracene and 2,3,7,8-tetrachlorodibenzo-p-dioxin, by phenobarbital, or by isoproterenol. Induction of the transferase by most compounds is maximal by 48 hours; however, upon treatment with 2,3,7,8-tetrachlorodibenzo-p-dioxin or 3-methylcholanthrene, the activity rises for at least 72 hours. Certain hydroxylated metabolities of benzo[a]pyrene, at the same concentration as used for benzo[a]pyrene, are shown to be inferior inducers of activity. Available hydroxylated metabolites of benzo[a]anthracene or 3-methyicholanthrene do not induce transferase activity. Combinations of a polycyclic aromatic compound and phenobarbital—at their optimal concentrations when added individually—cause additive induction of transferase activity whereas combinations of two polycyclic aromatic compounds do not cause greater activity than the best inducer alone. The induction of activity by the best inducer, 2,3,7,8-tetrachlorodibenzo-p-dioxin, is progressively inhibited by increasing concentrations of either actinomycin D or cycloheximide. In contrast, if cells are first exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin and cycloheximide for a period of time and then washed, treatment with actinomycin D causes an unusually rapid rise in transferase activity, as compared with that in cells transferred to control medium. The Reuber H-4-II-E cultures therefore contain a UDP-glucuronosyltransferase activity which is inducible by the three different classes of inducers already known to exist for monooxygenase activity in cell culture (2). Furthermore, substrates for UDP-glucuronosyltransferase(s) do not appear to function as very efficient inducers of the transferase activity.

  • Copyright © 1979 by Academic Press, Inc.

MolPharm articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Molecular Pharmacology
Vol. 16, Issue 3
1 Nov 1979
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Back Matter (PDF)
  • Editorial Board (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Molecular Pharmacology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Induction of UDP-Glucuronosyltransferase Activity in the Reuber H-4-II-E Hepatoma Cell Culture
(Your Name) has forwarded a page to you from Molecular Pharmacology
(Your Name) thought you would be interested in this article in Molecular Pharmacology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleArticle

Induction of UDP-Glucuronosyltransferase Activity in the Reuber H-4-II-E Hepatoma Cell Culture

NAJMA MALIK, GLENN M. KOTEEN and IDA S. OWENS
Molecular Pharmacology November 1, 1979, 16 (3) 950-960;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Research ArticleArticle

Induction of UDP-Glucuronosyltransferase Activity in the Reuber H-4-II-E Hepatoma Cell Culture

NAJMA MALIK, GLENN M. KOTEEN and IDA S. OWENS
Molecular Pharmacology November 1, 1979, 16 (3) 950-960;
del.icio.us logo Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • The binding site for KCI807 in the androgen receptor
  • Fatty acid amide hydrolase in cisplatin nephrotoxicity
  • eCB Signaling System in hiPSC-Derived Neuronal Cultures
Show more Articles

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About Molecular Pharmacology
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Journal of Pharmacology and Experimental Therapeutics
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0111 (Online)

Copyright © 2023 by the American Society for Pharmacology and Experimental Therapeutics