Abstract
In this report we present evidence that trifluorothymidine-5'-triphosphate (F3dTTP) once incorporated into activated calf thymus DNA by a partially purified HeLa cell cytoplasmic DNA-dependent DNA polymerase α does not lead to chain termination. Less than 1% of the [3H]F3dTTP incorporated in the cell-free system was recovered at the 3'-termini. In addition, F3dTTP-containing DNA was capable of incorporating [3H]dTTP, which led to the complete disappearance of radioactive trifluorothymidine from the 3'-termini.
ACKNOWLEDGMENTS The authors acknowledge Ms. Virginia Garcia for her technical assistance and Mrs. Sharon Garrison for the preparation of the manuscript.
- Copyright © 1980 by The American Society for Pharmacology and Experimental Therapeutics
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