Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Molecular Pharmacology
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Molecular Pharmacology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit molpharm on Facebook
  • Follow molpharm on Twitter
  • Follow molpharm on LinkedIn
Research ArticleArticle

Association of Alkali Metal and Alkaline Earth Cations with Subcellular Particles Prepared from Renal Cortex

MACKENZIE WALSER and VICTOR E. NAHMOD
Molecular Pharmacology February 1966, 2 (1) 10-21;
MACKENZIE WALSER
Department of Pharmacology and Experimental Therapeutics and Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
VICTOR E. NAHMOD
Department of Pharmacology and Experimental Therapeutics and Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

A differential centrifugation procedure was used to separate homogenized renal cortex of normal dogs into four sediments and four supernatants, each of which was analyzed for alkali and alkaline earth cations and for dry weight. The amount of each of the cations associated with, or "bound" by, the particles sedimenting in each step was computed by a method which takes into account trapped supernatant. Trace cations were determined radiometrically, except for lithium, which was administered by priming injection and constant infusion for 2 hr prior to nephrectomy. The extent of association of each cation with each of the four types of particles was expressed as the percentage of total tissue cation sedimenting with those particles. For the largest ("nuclear") particles, the extent of association of five alkali cations averaged 5.6%, and of four alkaline earths (Mg, Ca, Sr, and Ba) averaged 24%; no selectivity was seen in either group. The second largest ("mitochondrial") particles showed pronounced selectivity, the extent of association increasing from 2% to 15% with increasing atomic weight in the first group, and from 19% to 70% in the second. The third ("heavy microsomal") fraction showed no selectivity; an average of 1.4% of the alkaline cations and 6.7% of the alkaline earths was associated with these particles. The fourth ("light microsomal") fraction showed the following selectivity in order of increasing atomic weight: Li = Na > K < Rb = Cs; Mg> Ca> Sr « Ba.

Thus the "mitochondrial" particles preferentially adsorb (or take up during homogenization) larger cations in both groups, while the "light microsomal" particles show least binding of cations of intermediate size. The sequence of tissue-to-plasma ratios of alkaline earths was similar to the sequence of their association with the "light microsomal" particles. For alkali cations, tissue-to-plasma ratios paralleled the extent of their association with the "mitochondrial" particles.

ACKNOWLEDGMENTS This work was supported by a U. S. Public Health Service Research Grant (AM02306). M. Walser was supported by the U. S. Public Health Service Research Career Award Program (GM-K3-2583). V. E. Nahmod was supported by a Lederle International Fellowship.

  • Copyright ©, 1966, by Academic Press Inc.

MolPharm articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Molecular Pharmacology
Vol. 2, Issue 1
1 Feb 1966
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Editorial Board (PDF)
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Molecular Pharmacology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Association of Alkali Metal and Alkaline Earth Cations with Subcellular Particles Prepared from Renal Cortex
(Your Name) has forwarded a page to you from Molecular Pharmacology
(Your Name) thought you would be interested in this article in Molecular Pharmacology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleArticle

Association of Alkali Metal and Alkaline Earth Cations with Subcellular Particles Prepared from Renal Cortex

MACKENZIE WALSER and VICTOR E. NAHMOD
Molecular Pharmacology February 1, 1966, 2 (1) 10-21;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Research ArticleArticle

Association of Alkali Metal and Alkaline Earth Cations with Subcellular Particles Prepared from Renal Cortex

MACKENZIE WALSER and VICTOR E. NAHMOD
Molecular Pharmacology February 1, 1966, 2 (1) 10-21;
del.icio.us logo Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • The binding site for KCI807 in the androgen receptor
  • Fatty acid amide hydrolase in cisplatin nephrotoxicity
  • eCB Signaling System in hiPSC-Derived Neuronal Cultures
Show more Articles

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About Molecular Pharmacology
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Journal of Pharmacology and Experimental Therapeutics
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0111 (Online)

Copyright © 2023 by the American Society for Pharmacology and Experimental Therapeutics