Abstract
Dog and rat liver contain carbonic anhydrase in supernatant and particulate fractions. Hepatic bile of both species has higher HC03- and lower Cl- concentration than plasma. The enzyme in all fractions of dog liver was inhibited by unsubstituted aromatic sulfonamides at concentrations of 10-6 to 10-9 M. In vivo, administration of these drugs at doses which yield > 99% enzyme inhibition lowered biliary HCO3- and raised biliary Cl- concentrations. Certain of the drugs were secreted by liver and concentrated in the bile of both species. Rat liver supernatant carbonic anhydrase was nearly refractory to sulfonamide inhibition in vitro; this is the first example of such lowered response in the animal kingdom. Biliary electrolytes in the rat were accordingly not altered by high doses of these sulfonamides.
- Copyright ©, 1966, by Academic Press Inc.
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