Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Molecular Pharmacology
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Molecular Pharmacology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit molpharm on Facebook
  • Follow molpharm on Twitter
  • Follow molpharm on LinkedIn
Research ArticleArticle

Control of Ammonia Formation From ATP in a Multienzyme System of Liver in Presence of Uncouplers of Oxidative Phosphorylation

ERNEST KUN, HORACE H. LOH and S. B. I. EL-FIKY
Molecular Pharmacology September 1966, 2 (5) 481-490;
ERNEST KUN
Departments of Pharmacology and Biochemistry and the Cardiovascular Research Institute, School of Medicine, University of California, San Francisco Medical Center, San Francisco, California 94122
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
HORACE H. LOH
Departments of Pharmacology and Biochemistry and the Cardiovascular Research Institute, School of Medicine, University of California, San Francisco Medical Center, San Francisco, California 94122
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
S. B. I. EL-FIKY
Departments of Pharmacology and Biochemistry and the Cardiovascular Research Institute, School of Medicine, University of California, San Francisco Medical Center, San Francisco, California 94122
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

An in vitro system composed of isolated rat liver mitochondria and an AMP deaminase-containing cytoplasmic protein fraction has been employed for the study of the effect of uncouplers on the enzymic conversion of ATP to IMP + NH4+. Under these conditions one main pathway of ATP degradation is operative. It was shown that this metabolic sequence is initiated by activation of latent mitochondrial ATPase by uncouplers. Under uncoupled conditions, mitochondrial adenylate kinase converts ADP to AMP, which is almost quantitatively converted to IMP + NH4+ by cytoplasmic AMP deaminase. Oligomycin, which has no effect on AMP deaminase or adenylate kinase but counteracts the activating effect of uncouplers on mitochondrial ATPase, inhibits the ATP → IMP + NH4+ pathway by blocking the initiating step. An ATP-dependent activation of AMP-deaminase by Mg++ has been demonstrated. Relevance of these mechanisms to molecular control of cellular events is discussed.

ACKNOWLEDGMENTS This work was supported by research grants of the U.S. Public Health Service, RO1-CA-07955-03 and RO1-HD-01239-10, and of the National Science Foundation, GB-3488, and in part by U.S. Public Health Service training grant 2-T1-GM-475-06 (to Dr. El-Fiky).

  • Copyright ©, 1966, by Academic Press Inc.

MolPharm articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Molecular Pharmacology
Vol. 2, Issue 5
1 Sep 1966
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Editorial Board (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Molecular Pharmacology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Control of Ammonia Formation From ATP in a Multienzyme System of Liver in Presence of Uncouplers of Oxidative Phosphorylation
(Your Name) has forwarded a page to you from Molecular Pharmacology
(Your Name) thought you would be interested in this article in Molecular Pharmacology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleArticle

Control of Ammonia Formation From ATP in a Multienzyme System of Liver in Presence of Uncouplers of Oxidative Phosphorylation

ERNEST KUN, HORACE H. LOH and S. B. I. EL-FIKY
Molecular Pharmacology September 1, 1966, 2 (5) 481-490;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Research ArticleArticle

Control of Ammonia Formation From ATP in a Multienzyme System of Liver in Presence of Uncouplers of Oxidative Phosphorylation

ERNEST KUN, HORACE H. LOH and S. B. I. EL-FIKY
Molecular Pharmacology September 1, 1966, 2 (5) 481-490;
Reddit logo Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Fatty Acid Amide Hydrolase in Cisplatin Nephrotoxicity
  • Use-Dependent Relief of A-887826 Inhibition
  • Benzbromarone Relaxes Airway Smooth Muscle via BK Activation
Show more Articles

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About Molecular Pharmacology
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Journal of Pharmacology and Experimental Therapeutics
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0111 (Online)

Copyright © 2023 by the American Society for Pharmacology and Experimental Therapeutics