Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Molecular Pharmacology
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Molecular Pharmacology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit molpharm on Facebook
  • Follow molpharm on Twitter
  • Follow molpharm on LinkedIn
Abstract

Rat parotid gland protein kinase activation. Relationship to enzyme secretion.

T N Spearman and F R Butcher
Molecular Pharmacology January 1982, 21 (1) 121-127;
T N Spearman
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
F R Butcher
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

The degree of activation of rat parotid gland cyclic AMP-dependent protein kinase (EC 2.7.1.37) was measured in tissue minces in vitro in order to assess the involvement of this enzyme in the parotid stimulus-secretion coupling mechanism. Kinase activation, determined by the activity ratio method, was measurably increased by isoproterenol, a beta-adrenergic agonist and a potent stimulator of alpha-amylase (EC 3.2.1.1) secretion. Muscarinic cholinergic and alpha-adrenergic stimulation, less effective in releasing amylase, did not affect protein kinase activation. Kinase activation closely paralleled the cyclic AMP concentration when the concentration of isoproterenol was varied. Amylase release exhibited a similar isoproterenol dose-dependence, except that amylase release was measurably increased at an isoproterenol concentration slightly lower than that required to increase detectably the cyclic AMP concentration or kinase activation. Partial dissociation between cyclic AMP levels, kinase activation, and secretion was seen when submaximal beta-adrenergic stimulation was combined with submaximal and supramaximal cholinergic stimulation. These results suggest an involvement of cyclic AMP-dependent protein kinase in beta-adrenergic-stimulated amylase release, but show that the extent of secretion is not rigidly coupled to the extent of kinase activation as determined by the activity ratio method. Protein kinase activation may function in concert with other factors in the regulation of exocytosis in this tissue.

MolPharm articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Molecular Pharmacology
Vol. 21, Issue 1
1 Jan 1982
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Back Matter (PDF)
  • Editorial Board (PDF)
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Molecular Pharmacology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Rat parotid gland protein kinase activation. Relationship to enzyme secretion.
(Your Name) has forwarded a page to you from Molecular Pharmacology
(Your Name) thought you would be interested in this article in Molecular Pharmacology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Abstract

Rat parotid gland protein kinase activation. Relationship to enzyme secretion.

T N Spearman and F R Butcher
Molecular Pharmacology January 1, 1982, 21 (1) 121-127;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Abstract

Rat parotid gland protein kinase activation. Relationship to enzyme secretion.

T N Spearman and F R Butcher
Molecular Pharmacology January 1, 1982, 21 (1) 121-127;
Reddit logo Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About Molecular Pharmacology
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Journal of Pharmacology and Experimental Therapeutics
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0111 (Online)

Copyright © 2023 by the American Society for Pharmacology and Experimental Therapeutics