Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Molecular Pharmacology
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Molecular Pharmacology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit molpharm on Facebook
  • Follow molpharm on Twitter
  • Follow molpharm on LinkedIn
Abstract

Muscarinic cholinergic receptors on cultured thyroid cells. I. Biological effect of carbachol and characterization of the receptors.

S Champion and J Mauchamp
Molecular Pharmacology January 1982, 21 (1) 66-72;
S Champion
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
J Mauchamp
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

A muscarinic cholinergic effect on thyrotropin-stimulated cyclic AMP accumulation in cultured porcine thyroid cells is characterized. The muscarinic agonists carbachol, acetylcholine, oxotremorine, and pilocarpine decreased the acute cyclic AMP response to stimulation with thyrotropin (20 mU/ml). A 50% decrease was obtained in the presence of 0.1 mM carbachol. Maximal effects were observed when cells were cultured in the presence of thyrotropin (100 microU/ml) or prostaglandin E2 (1 microM), whereas cells cultured in basal medium or in the presence of dibutyryl cyclic AMP showed a low response to carbachol. Evidence is reported suggesting that carbachol acutely decreases cyclic AMP synthesis. The properties of the muscarinic receptors present in thyroid cells were defined by using the binding of the muscarinic antagonist [3H]quinuclidinyl benzilate to cell homogenates. Scatchard plots revealed a single population of binding sites with a KD of 0.3 nM. The numbers of binding sites per cell after 4 days in culture were 880 in control cells, 4335 in thyrotropin (100 microU/ml)-treated cells, 5600 in prostaglandin (1 microM)-treated cells, and 1420 in dibutyryl cyclic AMP-treated cells. Therefore the sensitivity to carbachol appeared to be related to the number of antagonist binding sites and to be independent of the sensitivity of the cells to acute thyrotropin stimulation.

MolPharm articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Molecular Pharmacology
Vol. 21, Issue 1
1 Jan 1982
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Back Matter (PDF)
  • Editorial Board (PDF)
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Molecular Pharmacology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Muscarinic cholinergic receptors on cultured thyroid cells. I. Biological effect of carbachol and characterization of the receptors.
(Your Name) has forwarded a page to you from Molecular Pharmacology
(Your Name) thought you would be interested in this article in Molecular Pharmacology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Abstract

Muscarinic cholinergic receptors on cultured thyroid cells. I. Biological effect of carbachol and characterization of the receptors.

S Champion and J Mauchamp
Molecular Pharmacology January 1, 1982, 21 (1) 66-72;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Abstract

Muscarinic cholinergic receptors on cultured thyroid cells. I. Biological effect of carbachol and characterization of the receptors.

S Champion and J Mauchamp
Molecular Pharmacology January 1, 1982, 21 (1) 66-72;
Reddit logo Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About Molecular Pharmacology
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Journal of Pharmacology and Experimental Therapeutics
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0111 (Online)

Copyright © 2023 by the American Society for Pharmacology and Experimental Therapeutics