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Molecular Pharmacology

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Abstract

Enkephalin biosynthesis in adrenal medulla. Modulation of proenkephalin mRNA content of cultured chromaffin cells by 8-bromo-adenosine 3',5'-monophosphate.

T T Quach, F Tang, H Kageyama, I Mocchetti, A Guidotti, J L Meek, E Costa and J P Schwartz
Molecular Pharmacology September 1984, 26 (2) 255-260;
T T Quach
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F Tang
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H Kageyama
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I Mocchetti
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A Guidotti
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J L Meek
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E Costa
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J P Schwartz
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Abstract

Incubation of primary cultures of chromaffin cells from bovine adrenal medulla with 8-bromo-adenosine 3',5'-monophosphate (8-Br-cyclic AMP) resulted in an increase in proenkephalin mRNA content. The mRNA that increased was detected by hybridization analysis using a cDNA probe and migrated with an apparent size of approximately 1400 bases. The increase in proenkephalin mRNA following 8-Br-cyclic AMP treatment was apparent in 12 hr and continued over 2 days. Corresponding changes were detected in enkephalin-like immunoreactivity but with a 24-hr lag: the cellular content increased significantly after 2 days of treatment and continued to rise over the next 2 days, whereas changes in the amount released to the medium followed the same time course. Dose-response curves for the increase in the content of proenkephalin mRNA and of enkephalin-containing peptides were essentially identical. Chromatographic characterization of the enkephalin-like peptides demonstrated that 8-Br-cyclic AMP increased both the high molecular weight fraction and the low molecular weight fraction, which was shown by high-pressure liquid chromatography to contain Met5-enkephalin, Leu5-enkephalin, and Met5-enkephalin-Arg6-Phe7. Previous results in chromaffin cells have demonstrated that the synthesis of tyrosine hydroxylase is also regulated by cyclic AMP, with a similar time course. These results therefore suggest the possibility of coordinate regulation by cyclic AMP of the expression of the cotransmitters, catecholamines and enkephalin peptides, in the adrenal medulla.

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Molecular Pharmacology
Vol. 26, Issue 2
1 Sep 1984
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Abstract

Enkephalin biosynthesis in adrenal medulla. Modulation of proenkephalin mRNA content of cultured chromaffin cells by 8-bromo-adenosine 3',5'-monophosphate.

T T Quach, F Tang, H Kageyama, I Mocchetti, A Guidotti, J L Meek, E Costa and J P Schwartz
Molecular Pharmacology September 1, 1984, 26 (2) 255-260;

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Abstract

Enkephalin biosynthesis in adrenal medulla. Modulation of proenkephalin mRNA content of cultured chromaffin cells by 8-bromo-adenosine 3',5'-monophosphate.

T T Quach, F Tang, H Kageyama, I Mocchetti, A Guidotti, J L Meek, E Costa and J P Schwartz
Molecular Pharmacology September 1, 1984, 26 (2) 255-260;
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