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Research ArticleArticle

Studies on Triiodothyronine-Induced Synthesis of Liver Mitochondrial α-Glycerophosphate Dehydrogenase in the Thyroidectomized Rat

KAI-LIN LEE and O. NEAL MILLER
Molecular Pharmacology January 1967, 3 (1) 44-51;
KAI-LIN LEE
Department of Biochemistry and the Nutrition and Metabolism Research Laboratory, Department of Medicine, Tulane University School of Medicine, New Orleans, Louisiana 70112
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O. NEAL MILLER
Department of Biochemistry and the Nutrition and Metabolism Research Laboratory, Department of Medicine, Tulane University School of Medicine, New Orleans, Louisiana 70112
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Abstract

Administration of a single small dose of triiodothymonine (T3) greatly increased liver mitochondrial L-α-glycerophosphate dehydrogenase activity of thyroidectomized rats.

The induction of liver mitochondrial L-α-glycerophoshate dehydrogenase by T3 could be blocked by simultaneous administration of puromycin; in addition, time incorporation of L-leucine-14C into total liver proteins and into proteins of all subcellular fractions was greatly inhibited. Furthermore, puromycin blocked further induction when it was administered after the administration of T3. L-Ethionine prevented the induction of enzyme formation, and the inhibition could be partially reversed by L-methionine. "Pulse labeling" was used to study the incorporation of L-leucine-14C into liver proteins, and the data indicate that an increased rate of protein synthesis precedes the maximal increase in liver mitochondrial L-α-glycerophosphate dehydrogenase produced by T3-administration. These observations suggest that T3-induction of L-α-glycerophosphate dehydrogenase in the thyroidectomized rat results from acceleration of enzyme synthesis. Similar observations were reported earlier with the euthyroid rat.

Administration of actinomycin D along with the T3 abolished the increase of L-α-glycerophosphate dehydrogenase in liver mitochondria. The induction was also partially inhibited by 5-fluorouracil. These results indicate that the induction process depends on the formation of an adequate amount of renewable messenger-RNA molecules.

ACKNOWLEDGMENTS This work was supported by a grant from the United States Public Health Service, TIGM-648-05. Time experimental data are taken from a dissertation submitted by Kai-Lin Lee to the Graduate School of Tulane University in partial fulfillment of the requirements for the degree of Doctor of Philosophy, 1966.

  • Copyright ©, 1967, by Academic Press Inc.

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Molecular Pharmacology
Vol. 3, Issue 1
1 Jan 1967
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Research ArticleArticle

Studies on Triiodothyronine-Induced Synthesis of Liver Mitochondrial α-Glycerophosphate Dehydrogenase in the Thyroidectomized Rat

KAI-LIN LEE and O. NEAL MILLER
Molecular Pharmacology January 1, 1967, 3 (1) 44-51;

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Research ArticleArticle

Studies on Triiodothyronine-Induced Synthesis of Liver Mitochondrial α-Glycerophosphate Dehydrogenase in the Thyroidectomized Rat

KAI-LIN LEE and O. NEAL MILLER
Molecular Pharmacology January 1, 1967, 3 (1) 44-51;
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