Abstract
Methylglyoxal-bis (guanylhydrazone) (CH3-G) increased the total uptake of glucose by epididymal fat pads of rats in vitro under aerobic, but not anaerobic, conditions. The following effects of CH3-G upon glycolytic pathways of adipose cells were found: (1) increased incorporation of radioactivity from glucose-14C into CO2 and lipid; (2) decreased incorporation of radioactivity from glucose-14C into glycogen; (3) increased incorporation of radioactivity from glucose-14C into released lactate; (4) decreased incorporation of radioactivity from acetate-1-14C into CO2 and lipid.
Under the same experimental conditions, CH3-G had no effect upon the rate of incorporation of radioactivity from glucose-14C into CO2 and lipid by epididymal fat pads of nfice.
The effects of phenformin upon glycolytic pathways of epididymal fat pads of rats in vitro were similar in some respects to CH3-G, but differed from the later in depressing incorporation of radioactivity from glucose-14C into CO2 and lipid. In addition, total glucose uptake was depressed by phenformin at the concentrations used.
The effects of CH3-G and phenformin differed from those of insulin with regard to the incorporation of radioactivity from glucose-14C into glycogen and incorporation from acetate-1-14C into CO2 and lipid. In addition, the effects of insulin were abolished in the presence of nethalide, whereas the effects of CH3-G were not. Insulin stimulated total uptake of glucose by both rat and mouse epididymal fat pads in vitro, but CH3-G had no effect upon the latter.
ACKNOWLEDGMENTS Supported by grants from the United States Public Health Service GM 209-07 and CA-08114-02.
- Copyright ©, 1967, by Academic Press Inc.
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