Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Molecular Pharmacology
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Molecular Pharmacology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit molpharm on Facebook
  • Follow molpharm on Twitter
  • Follow molpharm on LinkedIn
Abstract

Transformation of 5-hydroperoxyeicosatetraenoic acid into dihydroxy- and cysteinyl-leukotrienes by rat hepatocytes: effects of glutathione.

J Huwyler, M Bürgin, T Zeugin and J Gut
Molecular Pharmacology March 1991, 39 (3) 314-323;
J Huwyler
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
M Bürgin
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
T Zeugin
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
J Gut
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

In the presence of glutathione (GSH 400 microM), rat hepatocyte homogenates converted 5-hydroperoxyeicosatetraenoic acid (5-HPETE), via the intermediate leukotriene A4, into leukotriene C4 (LTC4) and leukotriene B4 (LTB4); 5-hydroxyeicosatetraenoic acid (5-HETE) was also a prominent product. During a 5-min incubation with 100 microM (13.4 microgram) 5-HPETE, 0.24 ng of LTC4, 15.4 ng of all-trans-LTB4, 4.3 ng of LTB4, and 12.4 micrograms of 5-HETE were formed/mg of protein. In incubations devoid of GSH, 38.6 ng of all-trans-LTB4, 8.8 ng of LTB4, and 2.2 micrograms of 5-HETE were formed/mg of protein, and 3.3 micrograms of intact 5-HPETE could be recovered. The presence of GSH induced a time-dependent rapid depletion of 5-HPETE, paralleled by large increases in the formation of 5-HETE; formation of LTC4 was detected in the presence but not in the absence of GSH. Addition of thiomalic acid (0.1 mM) or penicillamine (0.2 mM), both inhibitors of selenium-dependent GSH peroxidases, increased formation rates of LTC4 by factors of 3 and 2, respectively, whereas the suppressive effects of GSH on the formation of LTB4 were partially reversed. These results suggest that hepatocytes are capable of the simultaneous synthesis of cysteinyl- and dihydroxy-leukotrienes as well as 5-HETE; the availability of the precursor 5-HPETE and the profile of leukotrienes formed are dependent on the GSH concentration and the extent of GSH peroxidase activity.

MolPharm articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Molecular Pharmacology
Vol. 39, Issue 3
1 Mar 1991
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Back Matter (PDF)
  • Editorial Board (PDF)
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Molecular Pharmacology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Transformation of 5-hydroperoxyeicosatetraenoic acid into dihydroxy- and cysteinyl-leukotrienes by rat hepatocytes: effects of glutathione.
(Your Name) has forwarded a page to you from Molecular Pharmacology
(Your Name) thought you would be interested in this article in Molecular Pharmacology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Abstract

Transformation of 5-hydroperoxyeicosatetraenoic acid into dihydroxy- and cysteinyl-leukotrienes by rat hepatocytes: effects of glutathione.

J Huwyler, M Bürgin, T Zeugin and J Gut
Molecular Pharmacology March 1, 1991, 39 (3) 314-323;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Abstract

Transformation of 5-hydroperoxyeicosatetraenoic acid into dihydroxy- and cysteinyl-leukotrienes by rat hepatocytes: effects of glutathione.

J Huwyler, M Bürgin, T Zeugin and J Gut
Molecular Pharmacology March 1, 1991, 39 (3) 314-323;
del.icio.us logo Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About Molecular Pharmacology
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Journal of Pharmacology and Experimental Therapeutics
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0111 (Online)

Copyright © 2022 by the American Society for Pharmacology and Experimental Therapeutics