Abstract
The cocaine analog 2 beta-carboxymethoxy-3 beta-(4-fluorophenyl)-tropane (CFT) binds to platelet plasma membrane vesicles. [3H]CFT binding is blocked equally well by cocaine and imipramine. Specific (cocaine-sensitive) binding requires Na+ and is inhibited by H+ and Cl- ions. At 150 mM Na2SO4 and pH 9.5, the KD for [3H]CFT is 232 +/- 71 nM. The number of specific [3H]CFT binding sites on the membrane vesicles is equal to the number of serotonin transporters, as measured by [3H]imipramine binding. Binding of imipramine and CFT appeared to be mutually competitive. These results suggest that [3H]CFT and cocaine bind to the serotonin transporter at a site close to but distinct from the antidepressant binding site.
MolPharm articles become freely available 12 months after publication, and remain freely available for 5 years.Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page.
|
Log in using your username and password
Purchase access
You may purchase access to this article. This will require you to create an account if you don't already have one.
In this issue
Jump to section
Related Articles
Cited By...
Similar Articles
Advertisement