Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Molecular Pharmacology
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Molecular Pharmacology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit molpharm on Facebook
  • Follow molpharm on Twitter
  • Follow molpharm on LinkedIn
Abstract

Gender-related expression of rat microsomal epoxide hydrolase during maturation: post-transcriptional regulation.

S G Kim and Y H Kim
Molecular Pharmacology July 1992, 42 (1) 75-81;
S G Kim
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Y H Kim
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

Expression of microsomal epoxide hydrolase (mEH), levels of mEH mRNA, and the rate of mEH mRNA transcription were examined in hepatic and renal tissues at 4, 24, 44, and 56 weeks of age in male and 4, 14, 24, 34, and 44 weeks of age in female Sprague-Dawley rats. Immunoblot analyses revealed that hepatic mEH levels in males increased in an age-dependent manner, with a maximal increase (approximately 3-fold) being noted at 44 weeks of age, whereas the expression of hepatic mEH in females decreased significantly at 14 weeks of age or older, by approximately 70%s, compared with that of 4-week-old rats. Microsomes from kidney tissue failed to exhibit an age-dependent change in either sex. mEH mRNA levels were measured in total and poly(A)+ RNA isolated from hepatic and renal tissues. RNA blot hybridization analyses, probed with a 1.3-kilobase mEH cDNA, revealed that the levels of hepatic mEH mRNA in total RNA isolated from males were elevated approximately 1.5-, 2.8-, and 2.3-fold at 24, 44, and 56 weeks of age, respectively, relative to those at 4 weeks of age, whereas the levels of hepatic mEH mRNA in poly(A)+ RNA from males failed to change in an age-dependent manner. In contrast, the levels of hepatic mEH mRNA in either total or poly(A)+ RNA from female animals were dramatically decreased from 4 to 14 weeks of age, by approximately 90%. The suppressed levels of mEH mRNA in females were maintained at 24, 34, and 44 weeks of age (approximately 80%). However, the levels of renal mEH mRNA failed to change in an age-dependent manner in either sex, which was consistent with there being no change in the levels of mEH protein in kidney. In order to examine whether the gender-related maturational changes in hepatic mEH mRNA levels could result from transcriptional regulation, nuclear run-on assays were performed. The rate of hepatic mEH gene transcription failed to change in either males or females at the ages that exhibited significant changes in both mRNA levels and protein expression, suggesting that transcriptional regulation is not associated with the gender-dependent modulation of mEH mRNA levels during maturation.(ABSTRACT TRUNCATED AT 400 WORDS)

MolPharm articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Molecular Pharmacology
Vol. 42, Issue 1
1 Jul 1992
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Back Matter (PDF)
  • Editorial Board (PDF)
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Molecular Pharmacology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Gender-related expression of rat microsomal epoxide hydrolase during maturation: post-transcriptional regulation.
(Your Name) has forwarded a page to you from Molecular Pharmacology
(Your Name) thought you would be interested in this article in Molecular Pharmacology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Abstract

Gender-related expression of rat microsomal epoxide hydrolase during maturation: post-transcriptional regulation.

S G Kim and Y H Kim
Molecular Pharmacology July 1, 1992, 42 (1) 75-81;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Abstract

Gender-related expression of rat microsomal epoxide hydrolase during maturation: post-transcriptional regulation.

S G Kim and Y H Kim
Molecular Pharmacology July 1, 1992, 42 (1) 75-81;
Reddit logo Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About Molecular Pharmacology
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Journal of Pharmacology and Experimental Therapeutics
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0111 (Online)

Copyright © 2023 by the American Society for Pharmacology and Experimental Therapeutics