Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Molecular Pharmacology
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Molecular Pharmacology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Visit molpharm on Facebook
  • Follow molpharm on Twitter
  • Follow molpharm on LinkedIn
Abstract

Atypical low density lipoprotein binding site that may mediate lipoprotein-induced signal transduction.

V A Tkachuk, Y S Kuzmenko, T J Resink, D V Stambolsky and V N Bochkov
Molecular Pharmacology December 1994, 46 (6) 1129-1137;
V A Tkachuk
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Y S Kuzmenko
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
T J Resink
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
D V Stambolsky
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
V N Bochkov
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

The characteristics of low density lipoprotein (LDL) binding in quiescent cultures of human vascular smooth muscle cells (VSMC) have been further investigated and compared with the characteristics of high affinity LDL binding in human fibroblasts [via the apolipoprotein (apo) B/E receptor] and with the properties of LDL-induced phosphoinositide catabolism in VSMC. In VSMC the bulk of specific 125I-LDL binding occurs at a low affinity site, several characteristics of which are distinct from those of 125I-LDL binding to the apo B/E receptor in fibroblasts. (a) The affinity of LDL binding in VSMC is 25-50 times lower than that in fibroblasts (Kd approximately 50 micrograms/ml versus Kd approximately 2 micrograms/ml). (b) The kinetics of LDL association and dissociation in VSMC are more rapid than those in fibroblasts. (c) In contrast to apo B/E receptor-mediated binding of LDL in fibroblasts, binding of LDL to VSMC is insensitive to heparin, chemical modification of lysine residues, and chelation (with EDTA) of divalent cations. (d) Apo E-free high density lipoprotein 3 displaces labeled LDL more effectively in VSMC than in fibroblasts. (e) The ratio of bound/internalized LDL to degraded LDL differs markedly between fibroblasts and VSMC. LDL-stimulated phosphoinositide catabolism in VSMC, which occurs with an activation constant similar to the Kd for low affinity LDL binding, is insensitive to heparin, modification of lysine and arginine residues in LDL, and chelation of divalent cations. Thus, the atypical low affinity receptor in these cells may mediate the effects of LDL on signal transduction.

MolPharm articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Molecular Pharmacology
Vol. 46, Issue 6
1 Dec 1994
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Back Matter (PDF)
  • Editorial Board (PDF)
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Molecular Pharmacology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Atypical low density lipoprotein binding site that may mediate lipoprotein-induced signal transduction.
(Your Name) has forwarded a page to you from Molecular Pharmacology
(Your Name) thought you would be interested in this article in Molecular Pharmacology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Abstract

Atypical low density lipoprotein binding site that may mediate lipoprotein-induced signal transduction.

V A Tkachuk, Y S Kuzmenko, T J Resink, D V Stambolsky and V N Bochkov
Molecular Pharmacology December 1, 1994, 46 (6) 1129-1137;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Abstract

Atypical low density lipoprotein binding site that may mediate lipoprotein-induced signal transduction.

V A Tkachuk, Y S Kuzmenko, T J Resink, D V Stambolsky and V N Bochkov
Molecular Pharmacology December 1, 1994, 46 (6) 1129-1137;
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

Similar Articles

  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About Molecular Pharmacology
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Journal of Pharmacology and Experimental Therapeutics
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0111 (Online)

Copyright © 2021 by the American Society for Pharmacology and Experimental Therapeutics