Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Molecular Pharmacology
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Molecular Pharmacology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit molpharm on Facebook
  • Follow molpharm on Twitter
  • Follow molpharm on LinkedIn
Abstract

Signal transduction pathways for B1 and B2 bradykinin receptors in bovine pulmonary artery endothelial cells.

J A Smith, C Webb, J Holford and G M Burgess
Molecular Pharmacology March 1995, 47 (3) 525-534;
J A Smith
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
C Webb
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
J Holford
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
G M Burgess
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

Bovine pulmonary artery endothelial (CPAE) cells respond to bradykinin, and it has been suggested that the receptors on these cells do not fall into the normal B1/B2 classification of bradykinin receptors [J. Pharmacol. Exp. Ther. 244:646-649 (1988)]. The present study describes a detailed characterization of the subtypes of bradykinin receptors on CPAE cells. The B1-selective agonist des-Arg9-bradykinin and the B2-selective agonist bradykinin both activated polyphosphoinositide phospholipase C (PLC), caused an elevation in cytosolic Ca2+ concentration ([Ca2+]i), and increased the rate of 45Ca2+ efflux in CPAE cells. The pharmacology of these responses was consistent with interactions with B1 and B2 receptors. The effects of maximal concentrations of bradykinin and des-Arg9-bradykinin on the activity of PLC in populations of cells were not additive, suggesting that the two subtypes were expressed on the same cells. Indeed, des-Arg9-bradykinin and bradykinin both evoked increases in [Ca2+]i in 85% of single cells tested. The Ca2+ entry blocker NiCl2 inhibited bradykinin-induced increases in [Ca2+]i and 45Ca2+ efflux. In contrast, NiCl2 did not inhibit the increase in 45Ca2+ efflux evoked by des-Arg9-bradykinin and induced oscillatory increases in [Ca2+]i in response to the B1 agonist. NiCl2 had no effect on [3H]inositol trisphosphate generation by either agonist, indicating that its inhibitory effects on bradykinin-mediated Ca2+ responses were distal to B2 receptor-induced activation of PLC. LaCl3 did not differentiate between the 45Ca2+ efflux responses evoked by bradykinin and des-Arg9-bradykinin, attenuating both to a similar degree. Bradykinin-induced [3H]inositol trisphosphate formation was desensitized after pretreatment with bradykinin, but the response to des-Arg9-bradykinin was unchanged. Pretreatment with the B1 agonist did not inhibit responses evoked by subsequent challenges with either des-Arg9-bradykinin or bradykinin. These results provide pharmacological evidence for the existence of two distinct bradykinin receptor subtypes (B1 and B2) on CPAE cells, with no evidence for heterologous desensitization. Although both subtypes operated similar signal transduction pathways, the Ca2+ responses evoked by the two receptors could be differentiated by NiCl2.

MolPharm articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Molecular Pharmacology
Vol. 47, Issue 3
1 Mar 1995
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Back Matter (PDF)
  • Editorial Board (PDF)
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Molecular Pharmacology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Signal transduction pathways for B1 and B2 bradykinin receptors in bovine pulmonary artery endothelial cells.
(Your Name) has forwarded a page to you from Molecular Pharmacology
(Your Name) thought you would be interested in this article in Molecular Pharmacology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Abstract

Signal transduction pathways for B1 and B2 bradykinin receptors in bovine pulmonary artery endothelial cells.

J A Smith, C Webb, J Holford and G M Burgess
Molecular Pharmacology March 1, 1995, 47 (3) 525-534;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Abstract

Signal transduction pathways for B1 and B2 bradykinin receptors in bovine pulmonary artery endothelial cells.

J A Smith, C Webb, J Holford and G M Burgess
Molecular Pharmacology March 1, 1995, 47 (3) 525-534;
del.icio.us logo Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About Molecular Pharmacology
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Journal of Pharmacology and Experimental Therapeutics
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0111 (Online)

Copyright © 2022 by the American Society for Pharmacology and Experimental Therapeutics