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Molecular Pharmacology

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Abstract

Inhibition of human interferon-gamma biosynthesis by an antisense RNA-expressing vector.

C M Boeve, A V Wyngaerd and M De Ley
Molecular Pharmacology January 1996, 49 (1) 58-62;
C M Boeve
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A V Wyngaerd
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M De Ley
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Abstract

We applied antisense RNA technology for reducing the level of human IFN-gamma (HuIFN-gamma) expression. An antisense RNA vector containing the full-length HuIFN-gamma cDNA in the opposite orientation was electroporated into cells constitutively producing very high levels of the cytokine. Approximately 53% of the resulting clones exhibited a specific HuIFN-gamma inhibition of an average of 95.5%. The results of reverse transcription-polymerase chain reaction and Northern blot analyses revealed that the antisense effect originated from a specific reduction of the targeted mRNA caused by antisense RNA expression. This very effective antisense RNA strategy can have possible therapeutic applications in treating diseases where HuIFN-gamma is known to play a negative role, such as in certain autoimmune diseases.

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Molecular Pharmacology
Vol. 49, Issue 1
1 Jan 1996
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Abstract

Inhibition of human interferon-gamma biosynthesis by an antisense RNA-expressing vector.

C M Boeve, A V Wyngaerd and M De Ley
Molecular Pharmacology January 1, 1996, 49 (1) 58-62;

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Abstract

Inhibition of human interferon-gamma biosynthesis by an antisense RNA-expressing vector.

C M Boeve, A V Wyngaerd and M De Ley
Molecular Pharmacology January 1, 1996, 49 (1) 58-62;
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