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Molecular Pharmacology

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Abstract

Coexpression with potassium channel subunits used to clone the Y2 receptor for neuropeptide Y.

J M Rimland, E P Seward, Y Humbert, E Ratti, D G Trist and R A North
Molecular Pharmacology March 1996, 49 (3) 387-390;
J M Rimland
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E P Seward
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Y Humbert
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E Ratti
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D G Trist
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R A North
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Abstract

Xenopus oocytes were injected with RNAs for the two inward-rectifier potassium channel subunits Kir3.1 (GIRK1) and Kir3.4 (rcKATP or CIR) in addition to RNA from the neuroblastoma cell line KAN-TS. Potassium currents were evoked by neuropeptide Y in oocytes injected with polyadenylated RNA or with cRNA from pools of a neuroblastoma (KAN-TS) cDNA library, and progressive subdivision of responding pools yielded a single cDNA. The encoded protein contains 381 amino acids, has the seven hydrophobic domains characteristic of G protein-coupled receptors, and is 31% identical to the Y1 receptor: potassium currents were induced by neuropeptide Y (EC50=60pm) and Y2-selective analogues. Coexpression with potassium channel subunits will be a generally useful method for the cloning of G protein-coupled receptors.

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Molecular Pharmacology
Vol. 49, Issue 3
1 Mar 1996
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Abstract

Coexpression with potassium channel subunits used to clone the Y2 receptor for neuropeptide Y.

J M Rimland, E P Seward, Y Humbert, E Ratti, D G Trist and R A North
Molecular Pharmacology March 1, 1996, 49 (3) 387-390;

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Abstract

Coexpression with potassium channel subunits used to clone the Y2 receptor for neuropeptide Y.

J M Rimland, E P Seward, Y Humbert, E Ratti, D G Trist and R A North
Molecular Pharmacology March 1, 1996, 49 (3) 387-390;
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