Abstract
The reduction of p-nitrobenzoate by an NADPH-dependent enzyme in liver microsomes is presumably mediated by cytochrome P-450. The reaction is inhibited by substances which cause type II spectral changes in liver microsomes, but not by those which cause type I spectral changes. Type II substances inhibit only about 50% of the nitroreductase activity in liver microsomes. The apparent Ki values for the inhibition of p-nitrobenzoate reduction are approximately double the K[unknown], values of the various type II substances studied. The evidence suggests that type II substances inhibit p-nitrobenzoate reduction by slowing the rate of cytochrome P-450 reduction.
- Copyright ©, 1969, by Academic Press Inc.
MolPharm articles become freely available 12 months after publication, and remain freely available for 5 years.Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page.
|
Log in using your username and password
Purchase access
You may purchase access to this article. This will require you to create an account if you don't already have one.