Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Molecular Pharmacology
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Molecular Pharmacology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit molpharm on Facebook
  • Follow molpharm on Twitter
  • Follow molpharm on LinkedIn
Research ArticleArticle

Changes in Michaelis and Spectral Constants for Aniline in Hepatic Microsomes from Phenobarbital-Treated Rats

A. M. GUARINO, T. E. GRAM, P. L. GIGON, F. E. GREENE and J. R. GILLETTE
Molecular Pharmacology March 1969, 5 (2) 131-136;
A. M. GUARINO
Laboratories of Chemical Pharmacology, National Cancer and Heart Institutes, National Institutes of Health, Bethesda, Maryland 20014
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
T. E. GRAM
Laboratories of Chemical Pharmacology, National Cancer and Heart Institutes, National Institutes of Health, Bethesda, Maryland 20014
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
P. L. GIGON
Laboratories of Chemical Pharmacology, National Cancer and Heart Institutes, National Institutes of Health, Bethesda, Maryland 20014
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
F. E. GREENE
Laboratories of Chemical Pharmacology, National Cancer and Heart Institutes, National Institutes of Health, Bethesda, Maryland 20014
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
J. R. GILLETTE
Laboratories of Chemical Pharmacology, National Cancer and Heart Institutes, National Institutes of Health, Bethesda, Maryland 20014
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

Recent work has suggested that the rate of hepatic microsomal drug metabolism may be correlated with either the cytochrome P-450 content or the magnitude of the spectral change caused by the addition of substrates to microsomal suspensions. Accordingly, the relationships between these factors were investigated in 0.9% NaCl- and phenobarbital-treated intact, sham-operated, and partially hepatectomized rats. In intact animals phenobarbital produced a 3-4-fold increase in both microsomal aniline hydroxylase activity and cytochrome P-450 content, whereas in sham-operated or partially hepatectomized rats it produced a 4-fold increase in cytochrome P-450 content but only doubled aniline hydroxylase activity. Although phenobarbital treatment doubled the binding constants (K[unknown]) for aniline in all groups, it increased the maximum absorbance change (Amax) produced by the additione of aniline to microsomes about 7-fold in unoperated animals but only about 4-fold in the operated groups.

It was found that the Michaelis constant (Km) and binding constant (K[unknown]) for aniline differ by a factor of 10 in microsomal preparations from intact animals. Phenobarbital treatment was found to elicit significant increases in both K[unknown], and Km for aniline. These changes in Km and K[unknown], imply that induction by phenobarbital may be associated with qualitative as well as quantitative changes in the hepatic microsomal aniline hydroxylase.

  • Copyright ©, 1969, by Academic Press Inc.

MolPharm articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Molecular Pharmacology
Vol. 5, Issue 2
1 Mar 1969
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Editorial Board (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Molecular Pharmacology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Changes in Michaelis and Spectral Constants for Aniline in Hepatic Microsomes from Phenobarbital-Treated Rats
(Your Name) has forwarded a page to you from Molecular Pharmacology
(Your Name) thought you would be interested in this article in Molecular Pharmacology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleArticle

Changes in Michaelis and Spectral Constants for Aniline in Hepatic Microsomes from Phenobarbital-Treated Rats

A. M. GUARINO, T. E. GRAM, P. L. GIGON, F. E. GREENE and J. R. GILLETTE
Molecular Pharmacology March 1, 1969, 5 (2) 131-136;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Research ArticleArticle

Changes in Michaelis and Spectral Constants for Aniline in Hepatic Microsomes from Phenobarbital-Treated Rats

A. M. GUARINO, T. E. GRAM, P. L. GIGON, F. E. GREENE and J. R. GILLETTE
Molecular Pharmacology March 1, 1969, 5 (2) 131-136;
Reddit logo Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Mechanism of the selective action of paraherquamide A
  • Fatty Acid Amide Hydrolase in Cisplatin Nephrotoxicity
  • Use-Dependent Relief of A-887826 Inhibition
Show more Articles

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About Molecular Pharmacology
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Journal of Pharmacology and Experimental Therapeutics
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0111 (Online)

Copyright © 2023 by the American Society for Pharmacology and Experimental Therapeutics