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Research ArticleArticle

Effects of Adenophostin-A and Inositol-1,4,5-trisphosphate on Cl− Currents in Xenopus laevis Oocytes

H. Criss Hartzell, Khaled Machaca and Yoshiyuki Hirayama
Molecular Pharmacology April 1997, 51 (4) 683-692; DOI: https://doi.org/10.1124/mol.51.4.683
H. Criss Hartzell
Department of Anatomy and Cell Biology, Emory University School of Medicine, Atlanta, Georgia 30322-3030
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Khaled Machaca
Department of Anatomy and Cell Biology, Emory University School of Medicine, Atlanta, Georgia 30322-3030
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Yoshiyuki Hirayama
Department of Anatomy and Cell Biology, Emory University School of Medicine, Atlanta, Georgia 30322-3030
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Abstract

Adenophostin-A, a novel compound isolated from cultures ofPenicillium brevicompactum, has been shown to stimulate Ca2+ release from inositol-1,4,5-trisphosphate (IP3)-sensitive Ca2+ stores in microsomal preparations, permeabilized cells, and lipid vesicles containing purified IP3 receptor. The purpose of the current study was to compare the effects of adenophostin-A and IP3 on Ca2+ release from stores and Ca2+ influx in intact Xenopus laevis oocytes. Ca2+ influx though store-operated Ca2+ channels and Ca2+release from stores were monitored by measuring two Ca2+-activated Cl− currents that can be used as real-time indicators of Ca2+ release and Ca2+ influx (ICl-1 and ICl-2, respectively). We find that high concentrations (final intraoocyte concentrations of 5–10 μm) of adenophostin-A and IP3 stimulate a large Ca2+ release from stores (as measured by ICl-1) followed by Ca2+ influx (as measured by ICl-2). Low concentrations (∼50 nm) of IP3 stimulate oscillations in Ca2+ release without stimulating Ca2+ influx. In contrast, low concentrations of adenophostin-A can stimulate Ca2+ influx without stimulating a large Ca2+ release. However, Ca2+ influx did not occur in the complete absence of Ca2+ release. Therefore, it is unlikely that adenophostin-A directly stimulates store-operated Ca2+ channels. We hypothesize that adenophostin-A releases Ca2+ from a subpopulation of stores that is tightly coupled to store-operated Ca2+ channels.

Footnotes

    • Received October 23, 1996.
    • Accepted December 20, 1996.
  • Send reprint requests to: Dr. H. Criss Hartzell, Department of Anatomy and Cell Biology, Room 333, 1648 Pierce Drive, Emory University School of Medicine, Atlanta, GA 30322-3030. E-mail:criss{at}anatomy.emory.edu

  • This work was supported by National Institutes of Health Grants HL54074 and GM55276.

  • The American Society for Pharmacology and Experimental Therapeutics
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Molecular Pharmacology: 51 (4)
Molecular Pharmacology
Vol. 51, Issue 4
1 Apr 1997
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Research ArticleArticle

Effects of Adenophostin-A and Inositol-1,4,5-trisphosphate on Cl− Currents in Xenopus laevis Oocytes

H. Criss Hartzell, Khaled Machaca and Yoshiyuki Hirayama
Molecular Pharmacology April 1, 1997, 51 (4) 683-692; DOI: https://doi.org/10.1124/mol.51.4.683

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Research ArticleArticle

Effects of Adenophostin-A and Inositol-1,4,5-trisphosphate on Cl− Currents in Xenopus laevis Oocytes

H. Criss Hartzell, Khaled Machaca and Yoshiyuki Hirayama
Molecular Pharmacology April 1, 1997, 51 (4) 683-692; DOI: https://doi.org/10.1124/mol.51.4.683
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