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Molecular Pharmacology

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Research ArticleArticle

Localization and Functional Expression of Splice Variants of the P2X2 Receptor

Joseph Simon, Emma J. Kidd, Fiona M. Smith, Iain P. Chessell, Ruth Murrell-Lagnado, Patrick P. A. Humphrey and Eric A. Barnard
Molecular Pharmacology August 1997, 52 (2) 237-248; DOI: https://doi.org/10.1124/mol.52.2.237
Joseph Simon
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Emma J. Kidd
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Fiona M. Smith
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Iain P. Chessell
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Ruth Murrell-Lagnado
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Patrick P. A. Humphrey
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Eric A. Barnard
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Abstract

cDNAs encoding three splice variants of the P2X2 receptor were isolated from rat cerebellum. The first variant has a serine/proline-rich segment deleted from the intracellularly located carboxyl-terminal domain of the P2X2 subunit. The second and third variants have the splice site in the second half of the predicted first transmembrane domain. Either a 12-amino acid insertion or a six-amino acid deletion occurs at this position. cRNAs for these isoforms of the P2X2 subunit were injected intoXenopus laevis oocytes and tested for function. ATP evoked inward currents only with the splice variant [designated P2X2(b)] having the 69-amino acid deletion. The potencies of various agonists at the homomeric P2X2(b) receptor were not significantly different from those at the P2X2(a)homomeric channel. However, the P2X2(b) receptor showed significantly lower antagonist sensitivity. In contrast to the nondesensitizing P2X2(a) receptor, prolonged application of ATP produced a more rapid desensitization of the P2X2(b)receptor. When the P2X2(a) and P2X2(b) receptor responses were recorded in transfected mammalian cells, this difference was again found. The change in desensitization may be determined by proline/serine-rich segments and/or phosphorylation motifs that are removed from the tail region in formation of the P2X2(b)subunit. In situ hybridization of the three newly isolated isoforms of the P2X2 subunit was performed at the macroscopic and cellular levels; transcripts for two of them [P2X2(b) and p2x2(c)] but not the third [p2x2(d)], which carries the 12-amino acid addition, were present in many structures in the neonatal rat brain and on sensory and sympathetic ganglia. mRNA for the p2x2(d) splice variant was present only in the nodose ganglion, at a low level.

Footnotes

    • Received December 26, 1996.
    • Accepted April 24, 1997.
  • Send reprint requests to: P. P. A. Humphrey, Glaxo Institute of Applied Pharmacology, Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QJ, UK. E-mail: ppah0562{at}ggr.co.uk

  • The American Society for Pharmacology and Experimental Therapeutics
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Molecular Pharmacology: 52 (2)
Molecular Pharmacology
Vol. 52, Issue 2
1 Aug 1997
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Research ArticleArticle

Localization and Functional Expression of Splice Variants of the P2X2 Receptor

Joseph Simon, Emma J. Kidd, Fiona M. Smith, Iain P. Chessell, Ruth Murrell-Lagnado, Patrick P. A. Humphrey and Eric A. Barnard
Molecular Pharmacology August 1, 1997, 52 (2) 237-248; DOI: https://doi.org/10.1124/mol.52.2.237

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Research ArticleArticle

Localization and Functional Expression of Splice Variants of the P2X2 Receptor

Joseph Simon, Emma J. Kidd, Fiona M. Smith, Iain P. Chessell, Ruth Murrell-Lagnado, Patrick P. A. Humphrey and Eric A. Barnard
Molecular Pharmacology August 1, 1997, 52 (2) 237-248; DOI: https://doi.org/10.1124/mol.52.2.237
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