Abstract

We examined the ability of different G protein subunits to inhibit the activity of human α1B and α1E Ca²⁺ channels stably expressed in human embryonic kidney (HEK) 293 cells together with β1B and α2Bδ Ca²⁺ channel subunits. Under normal conditions, Ca²⁺ currents in α1B-expressing cells showed little facilitation after a depolarizing prepulse. However, when we overexpressed the β2γ2 subunits of heterotrimeric G proteins, the time course of activation of the Ca²⁺ currents was considerably slowed and a depolarizing prepulse produced a large facilitation of the current as well as an acceleration in its time course of activation. Similar effects were not observed when cells were transfected with constitutively active mutants of the G protein α subunits αs, αi1, and αo or with the G protein β2 and γ2 subunits alone. Studies carried out in cells expressing α1E currents showed that overexpression of β2γ2 subunits produced prepulse facilitation, although this was of lesser magnitude than that observed with Ca²⁺ currents in α1B-expressing cells. The subunits β2 and γ2 alone produced no effects, nor did constitutively active αs, αi1, and αo subunits. Phorbol esters enhanced α1E Ca²⁺ currents but had no effect on α1B currents, suggesting that protein kinase C activation was not responsible for the observed effects. When α1E Ca²⁺ currents were expressed without their β subunits, they exhibited prepulse facilitation. These results demonstrate that α1E Ca²⁺ currents are less susceptible to direct modulation by G proteins than α1B currents and illustrate the antagonistic interactions between Ca²⁺channel β subunits and G proteins.