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Molecular Pharmacology

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Rapid CommunicationAccelerated Communication

Recombinant Human CXC-Chemokine Receptor-4 in Melanophores Are Linked to Gi Protein: Seven Transmembrane Coreceptors for Human Immunodeficiency Virus Entry into Cells

Wen-Ji Chen, Channa Jayawickreme, Chris Watson, Larry Wolfe, William Holmes, Robert Ferris, Susan Armour, Walter Dallas, Grace Chen, Larry Boone, Michael Luther and Terry Kenakin
Molecular Pharmacology February 1998, 53 (2) 177-181; DOI: https://doi.org/10.1124/mol.53.2.177
Wen-Ji Chen
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Channa Jayawickreme
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Chris Watson
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Larry Wolfe
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Robert Ferris
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Susan Armour
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Walter Dallas
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Grace Chen
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Michael Luther
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Terry Kenakin
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Abstract

This article describes the transient expression of the CXC chemokine receptor-4 in Xenopus laevis melanophores and the resulting functional assay for the endogenous ligand for this receptor stromal cell-derived factor (SDF)-1α. Specifically, it will be shown that SDF-1α produces increased light transmittance in transfected cells that is consistent with the activation of Gi protein. This stimulus pathway is further implicated by the abolition of this response after pretreatment of the cells with pertussis toxin, a known method for the inactivation of Giprotein. The fact that SDF-1α does not produce responses in nontransfected cells and that treatment of the cells with 12G5, an antibody specific for the CXC chemokine receptor-4, eliminates this response indicates that this ligand produces responses by activation of this receptor in these cells. The possible relevance to human immunodeficiency virus (HIV) entry into cells was explored by observing the effects of SDF-1α on HIV-mediated cell fusion. It was found that SDF-1α blocked cell-to-cell fusion (as has been previously reported) at concentrations 1200-fold greater than those required to produce Gi protein mediated responses. The implications of the functional assay to screening for new drugs to block HIV-mediated fusion is discussed.

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Molecular Pharmacology: 53 (2)
Molecular Pharmacology
Vol. 53, Issue 2
1 Feb 1998
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Rapid CommunicationAccelerated Communication

Recombinant Human CXC-Chemokine Receptor-4 in Melanophores Are Linked to Gi Protein: Seven Transmembrane Coreceptors for Human Immunodeficiency Virus Entry into Cells

Wen-Ji Chen, Channa Jayawickreme, Chris Watson, Larry Wolfe, William Holmes, Robert Ferris, Susan Armour, Walter Dallas, Grace Chen, Larry Boone, Michael Luther and Terry Kenakin
Molecular Pharmacology February 1, 1998, 53 (2) 177-181; DOI: https://doi.org/10.1124/mol.53.2.177

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Rapid CommunicationAccelerated Communication

Recombinant Human CXC-Chemokine Receptor-4 in Melanophores Are Linked to Gi Protein: Seven Transmembrane Coreceptors for Human Immunodeficiency Virus Entry into Cells

Wen-Ji Chen, Channa Jayawickreme, Chris Watson, Larry Wolfe, William Holmes, Robert Ferris, Susan Armour, Walter Dallas, Grace Chen, Larry Boone, Michael Luther and Terry Kenakin
Molecular Pharmacology February 1, 1998, 53 (2) 177-181; DOI: https://doi.org/10.1124/mol.53.2.177
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