Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Molecular Pharmacology
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Molecular Pharmacology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit molpharm on Facebook
  • Follow molpharm on Twitter
  • Follow molpharm on LinkedIn
Research ArticleArticle

The Rat Heme Oxygenase-1 Gene Is Transcriptionally Induced via the Protein Kinase A Signaling Pathway in Rat Hepatocyte Cultures

Stephan Immenschuh, Thomas Kietzmann, Vera Hinke, Matthias Wiederhold, Norbert Katz and Ursula Muller-Eberhard
Molecular Pharmacology March 1998, 53 (3) 483-491; DOI: https://doi.org/10.1124/mol.53.3.483
Stephan Immenschuh
Departments of 1 2 3
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Thomas Kietzmann
Departments of 1 2 3
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Vera Hinke
Departments of 1 2 3
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Matthias Wiederhold
Departments of 1 2 3
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Norbert Katz
Departments of 1 2 3
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ursula Muller-Eberhard
Departments of 1 2 3
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

Heme oxygenase-1 (HO-1) is the inducible form of the rate-limiting enzyme of heme degradation; it regulates the cellular content of heme. To investigate the role of the cAMP-dependent protein kinase (PKA) signaling pathway on hepatic HO-1 gene expression, primary rat hepatocyte cultures were treated with the PKA-stimulating agents dibutyryl-cAMP (Bt2cAMP), forskolin, and glucagon. HO-1 mRNA levels were induced by these agents in a time-dependent manner with a transient maximum after 6 hr of treatment. The induction of HO-1 was dose dependent, reaching a maximum at concentrations of 250 μm Bt2cAMP and 50 nm glucagon, respectively. The accumulation of HO-1 mRNA correlated with increased levels of HO-1 protein as determined by Western blot analysis. The Bt2cAMP-dependent induction of HO-1 mRNA expression was prevented by pretreatment with the PKA inhibitor KT5720 but not with the protein kinase G inhibitor KT5823. HO-1 mRNA induction by CdCl2 and heme was differentially affected by Bt2cAMP. Up-regulation of the HO-1 gene by Bt2cAMP occurred on the transcriptional level as determined by nuclear run-off assay and blocking of the Bt2cAMP-dependent induction of HO-1 mRNA by actinomycin D. Treatment with Bt2cAMP increased the half-life of HO-1 mRNA from 4.7 to 5.5 hr. Taken together, the results of the current study demonstrate that HO-1 gene expression is induced by activation of the cAMP signal transduction pathway via a transcriptional mechanism in primary rat hepatocyte cultures.

Footnotes

    • Received September 2, 1997.
    • Accepted December 4, 1997.
  • Send reprint requests to: Stephan Immenschuh, M.D., Zentrum für Innere Medizin, Abteilung Gastroenterologie und Endokrinologie, Georg-August Universität Göttingen, Robert-Koch Str. 40, 37075 Göttingen, Germany. E-mail:simmens{at}gwdg.de

  • ↵1 Current affiliation: Zentrum für Innere Medizin, Abteilung Gastroenterologie und Endokrinologie, Georg-August Universität Göttingen, 37075 Göttingen, Germany.

  • This study was supported by National Institutes of Health Grants DK30203 and DK30664 (U.M.-E.), the Children’s Blood Foundation of the New York Hospital (U.M.-E.), and Deutsche Forschungsgemeinschaft Grants Im 20/2–1 (S.I.) and SFB 402 (T.K.).

  • The American Society for Pharmacology and Experimental Therapeutics
View Full Text

MolPharm articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Molecular Pharmacology: 53 (3)
Molecular Pharmacology
Vol. 53, Issue 3
1 Mar 1998
  • Table of Contents
  • About the Cover
  • Index by author
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Molecular Pharmacology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
The Rat Heme Oxygenase-1 Gene Is Transcriptionally Induced via the Protein Kinase A Signaling Pathway in Rat Hepatocyte Cultures
(Your Name) has forwarded a page to you from Molecular Pharmacology
(Your Name) thought you would be interested in this article in Molecular Pharmacology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleArticle

The Rat Heme Oxygenase-1 Gene Is Transcriptionally Induced via the Protein Kinase A Signaling Pathway in Rat Hepatocyte Cultures

Stephan Immenschuh, Thomas Kietzmann, Vera Hinke, Matthias Wiederhold, Norbert Katz and Ursula Muller-Eberhard
Molecular Pharmacology March 1, 1998, 53 (3) 483-491; DOI: https://doi.org/10.1124/mol.53.3.483

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Research ArticleArticle

The Rat Heme Oxygenase-1 Gene Is Transcriptionally Induced via the Protein Kinase A Signaling Pathway in Rat Hepatocyte Cultures

Stephan Immenschuh, Thomas Kietzmann, Vera Hinke, Matthias Wiederhold, Norbert Katz and Ursula Muller-Eberhard
Molecular Pharmacology March 1, 1998, 53 (3) 483-491; DOI: https://doi.org/10.1124/mol.53.3.483
Reddit logo Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Experimental Procedures
    • Results
    • Discussion
    • Acknowledgments
    • Footnotes
    • Abbreviations
    • References
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Analgesic Effects and Mechanisms of Licochalcones
  • Induced Fit Ligand Binding to CYP3A4
  • Englerin A Inhibits T-Type Channels
Show more Article

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About Molecular Pharmacology
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Journal of Pharmacology and Experimental Therapeutics
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0111 (Online)

Copyright © 2023 by the American Society for Pharmacology and Experimental Therapeutics