Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Molecular Pharmacology
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Molecular Pharmacology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit molpharm on Facebook
  • Follow molpharm on Twitter
  • Follow molpharm on LinkedIn
Research ArticleArticle

Inhibition of Cell Surface Expression by Mutant Receptors Demonstrates that D2 Dopamine Receptors Exist as Oligomers in the Cell

Samuel P. Lee, Brian F. O'Dowd, Gordon Y.K. Ng, George Varghese, Huda Akil, Alfred Mansour, Tuan Nguyen and Susan R. George
Molecular Pharmacology July 2000, 58 (1) 120-128; DOI: https://doi.org/10.1124/mol.58.1.120
Samuel P. Lee
Departments of 1 2 3 4 5
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Brian F. O'Dowd
Departments of 1 2 3 4 5
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gordon Y.K. Ng
Departments of 1 2 3 4 5
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
George Varghese
Departments of 1 2 3 4 5
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Huda Akil
Departments of 1 2 3 4 5
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Alfred Mansour
Departments of 1 2 3 4 5
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tuan Nguyen
Departments of 1 2 3 4 5
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Susan R. George
Departments of 1 2 3 4 5
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

Numerous mutant G protein-coupled receptors with diminished or no function have been described that are naturally occurring or that are the product of gene manipulation. It has largely been assumed that receptor mutants do not affect the function of the wild-type receptor; however, the occurrence of G protein-coupled receptor dimerization suggests the possibility that an intermolecular interaction between mutant and wild-type receptors can occur. We have shown previously that the D2 dopamine receptor (D2DR) exists as dimers in cell lines and brain tissue. In this study, we demonstrated that mutant D2DR can modulate the function of the wild-type D2DR. While attempting to elucidate the structure of the D2DR dimer, we demonstrated that nonfunctional D2DR substitution and truncation mutants antagonized wild-type D2DR function. Furthermore, from analyses of this interaction between the receptor mutants and the D2DR, using photoaffinity labeling, we provide evidence that the D2DR is oligomeric in the cell.

Footnotes

    • Received November 5, 1999.
    • Accepted March 28, 2000.
  • Dr. Susan R. George, Department of Pharmacology, University of Toronto, Room 4358, Medical Sciences Blgd., 1 King's College Circle, Toronto, ON M5S 1A8, Canada. E-mail:s.george{at}utoronto.ca.

  • ↵1 Current address: Merck Frosst Center for Therapeutic Research, Kirkland, QC H9H 3L1, Canada.

  • ↵2 Current address: Pharmaco Genesis, 6628 Heather Heath Lane, West Bloomfield, MI, 48322.

  • This work was supported by grants from the Medical Research Council of Canada, the National Institute on Drug Abuse, the Smokeless Tobacco Research Council, an Addiction Research Foundation Fellowship to S.P.L., and a Medical Research Council of Canada Fellowship to G.N.

  • The American Society for Pharmacology and Experimental Therapeutics
View Full Text

MolPharm articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Molecular Pharmacology: 58 (1)
Molecular Pharmacology
Vol. 58, Issue 1
1 Jul 2000
  • Table of Contents
  • About the Cover
  • Index by author
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Molecular Pharmacology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Inhibition of Cell Surface Expression by Mutant Receptors Demonstrates that D2 Dopamine Receptors Exist as Oligomers in the Cell
(Your Name) has forwarded a page to you from Molecular Pharmacology
(Your Name) thought you would be interested in this article in Molecular Pharmacology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleArticle

Inhibition of Cell Surface Expression by Mutant Receptors Demonstrates that D2 Dopamine Receptors Exist as Oligomers in the Cell

Samuel P. Lee, Brian F. O'Dowd, Gordon Y.K. Ng, George Varghese, Huda Akil, Alfred Mansour, Tuan Nguyen and Susan R. George
Molecular Pharmacology July 1, 2000, 58 (1) 120-128; DOI: https://doi.org/10.1124/mol.58.1.120

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Research ArticleArticle

Inhibition of Cell Surface Expression by Mutant Receptors Demonstrates that D2 Dopamine Receptors Exist as Oligomers in the Cell

Samuel P. Lee, Brian F. O'Dowd, Gordon Y.K. Ng, George Varghese, Huda Akil, Alfred Mansour, Tuan Nguyen and Susan R. George
Molecular Pharmacology July 1, 2000, 58 (1) 120-128; DOI: https://doi.org/10.1124/mol.58.1.120
Reddit logo Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Materials and Methods
    • Results
    • Discussion
    • Acknowledgment
    • Footnotes
    • Abbreviations
    • References
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Cysteine151 in Keap1 Drives CDDO-Me Pharmacodynamic Action
  • Allosteric Modulation of Metabotropic Glutamate Receptor 1
  • Mechanism of Selective Action of Paraherquamide A
Show more Article

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About Molecular Pharmacology
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Journal of Pharmacology and Experimental Therapeutics
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0111 (Online)

Copyright © 2023 by the American Society for Pharmacology and Experimental Therapeutics