Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Molecular Pharmacology
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Molecular Pharmacology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit molpharm on Facebook
  • Follow molpharm on Twitter
  • Follow molpharm on LinkedIn
Research ArticleArticle

Evidence for Edg-3 Receptor-Mediated Activation ofI K.ACh by Sphingosine-1-Phosphate in Human Atrial Cardiomyocytes

Herbert M. Himmel, Dagmar Meyer zu Heringdorf, Eva Graf, Dobromir Dobrev, Ariane Kortner, Stephan Schüler, Karl H. Jakobs and Ursula Ravens
Molecular Pharmacology August 2000, 58 (2) 449-454; DOI: https://doi.org/10.1124/mol.58.2.449
Herbert M. Himmel
1 2 3
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dagmar Meyer zu Heringdorf
1 2 3
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Eva Graf
1 2 3
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dobromir Dobrev
1 2 3
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ariane Kortner
1 2 3
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Stephan Schüler
1 2 3
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Karl H. Jakobs
1 2 3
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ursula Ravens
1 2 3
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

Sphingosine-1-phosphate (SPP) and sphingosylphosphorylcholine (SPPC) have been reported to activate muscarinic receptor-activated inward rectifier K+ current (I K.ACh) in cultured guinea pig atrial myocytes with similar nanomolar potency. Members of the endothelial differentiation gene (Edg) receptor family were recently identified as receptors for SPP; however, these receptors respond only to micromolar concentrations of SPPC. Here we investigated the sphingolipid-induced activation ofI K.ACh in freshly isolated guinea pig, mouse, and human atrial myocytes. SPP activatedI K.ACh in atrial myocytes from all three species with a similar nanomolar potency (EC50 values: 4–8 nM). At these low concentrations, SPPC also activatedI K.ACh in guinea pig myocytes. In contrast, SPPC was almost ineffective in mouse and human myocytes, thus resembling the pharmacology of the Edg receptors. Transcripts ofEdg-1, Edg-3, and Edg-5were detected in human atrial cells. Moreover, activation ofI K.ACh by SPP was blocked by the Edg-3-selective antagonist suramin, which did not affect basal or carbachol-stimulated K+ currents. In conclusion, these data indicate that I K.ACh activation by SPP and SPPC exhibits large species differences. Furthermore, they suggest that SPP-induced I K.ACh activation in human atrial myocytes is mediated by the Edg-3 subtype of SPP receptors.

Footnotes

    • Received December 29, 1999.
    • Accepted May 16, 2000.
  • Send reprint requests to: Dr. Herbert M. Himmel, Institut für Pharmakologie und Toxikologie, Medizinische Fakultät Carl Gustav Carus, TU Dresden, Karl-Marx-Straβe 3, D-01109 Dresden, Germany. E-mail: himmel{at}rcs.urz.tu-dresden.de

  • ↵1 These authors contributed equally to this work.

  • This work was supported in part by the Deutsche Forschungsgemeinschaft (Grant ME 1734/1 to D.M.z.H.).

  • The American Society for Pharmacology and Experimental Therapeutics
View Full Text

MolPharm articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Molecular Pharmacology: 58 (2)
Molecular Pharmacology
Vol. 58, Issue 2
1 Aug 2000
  • Table of Contents
  • About the Cover
  • Index by author
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Molecular Pharmacology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Evidence for Edg-3 Receptor-Mediated Activation ofI K.ACh by Sphingosine-1-Phosphate in Human Atrial Cardiomyocytes
(Your Name) has forwarded a page to you from Molecular Pharmacology
(Your Name) thought you would be interested in this article in Molecular Pharmacology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleArticle

Evidence for Edg-3 Receptor-Mediated Activation ofI K.ACh by Sphingosine-1-Phosphate in Human Atrial Cardiomyocytes

Herbert M. Himmel, Dagmar Meyer zu Heringdorf, Eva Graf, Dobromir Dobrev, Ariane Kortner, Stephan Schüler, Karl H. Jakobs and Ursula Ravens
Molecular Pharmacology August 1, 2000, 58 (2) 449-454; DOI: https://doi.org/10.1124/mol.58.2.449

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Research ArticleArticle

Evidence for Edg-3 Receptor-Mediated Activation ofI K.ACh by Sphingosine-1-Phosphate in Human Atrial Cardiomyocytes

Herbert M. Himmel, Dagmar Meyer zu Heringdorf, Eva Graf, Dobromir Dobrev, Ariane Kortner, Stephan Schüler, Karl H. Jakobs and Ursula Ravens
Molecular Pharmacology August 1, 2000, 58 (2) 449-454; DOI: https://doi.org/10.1124/mol.58.2.449
del.icio.us logo Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Materials and Methods
    • Results
    • Discussion
    • Acknowledgments
    • Footnotes
    • Abbreviations
    • References
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Anti-aromatase activity of exemestane phase II metabolites
  • α-Conotoxin Binding Site on the GABAB Receptor
  • Upacicalcet binds to the amino acid binding site of CaSR
Show more Article

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About Molecular Pharmacology
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Journal of Pharmacology and Experimental Therapeutics
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0111 (Online)

Copyright © 2022 by the American Society for Pharmacology and Experimental Therapeutics