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Molecular Pharmacology

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Research ArticleArticle

Differential Fibronectin Expression in Activated C6 Glial Cells Treated with Ethanol

Li Q. Ren, Daniel K. Garrett, Marie Syapin and Peter J. Syapin
Molecular Pharmacology December 2000, 58 (6) 1303-1309; DOI: https://doi.org/10.1124/mol.58.6.1303
Li Q. Ren
Alcohol and Brain Research Laboratory, Department of Pharmacology, Texas Tech University Health Sciences Center, Lubbock, Texas
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Daniel K. Garrett
Alcohol and Brain Research Laboratory, Department of Pharmacology, Texas Tech University Health Sciences Center, Lubbock, Texas
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Marie Syapin
Alcohol and Brain Research Laboratory, Department of Pharmacology, Texas Tech University Health Sciences Center, Lubbock, Texas
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Peter J. Syapin
Alcohol and Brain Research Laboratory, Department of Pharmacology, Texas Tech University Health Sciences Center, Lubbock, Texas
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Abstract

The central nervous system is particularly susceptible to alcohol effects and toxicity. Glial cells constitute the most common cell type in the brain and play critical roles in normal brain function and during infection and injury. Astrocytes in particular seem to be important targets for alcohol neurotoxicity during both development and in adulthood. To gain more insight into alcohol-mediated effects on astrocytes at the molecular level, gene expression in rat C6 glial cells was studied in the presence or absence of ethanol. The differential display of mRNA technique was used to screen the expressed genes in ethanol-treated rat C6 cells before and after treatment with lipopolysaccharide (LPS) combined with phorbol-12-myristate-13-acetate (PMA), conditions that mimic an infectious inflammatory state and cause immunologic activation. The present data show that fibronectin appeared as a major gene whose expression is increased in C6 cells by LPS plus PMA stimulation and decreased by chronic ethanol exposure, both in mRNA and protein levels. Fibronectin is a dimeric glycoprotein found in the extracellular matrix of most tissues, in the blood, and on cell surfaces and is involved in many cellular processes. These results show that chronic exposure to ethanol is associated with changes in astrocyte properties during immunologic activation that reduce fibronectin expression. The discovery of astrocyte fibronectin expression as a potential regulated target for chronic alcohol abuse may be useful in understanding, preventing, and treating some brain disorders associated with alcohol abuse and alcoholism.

Footnotes

    • Received February 11, 2000.
    • Accepted August 15, 2000.
  • Send reprint requests to: Send reprint requests to: Dr Peter J Syapin, Texas Tech University Health Sciences Center, 3601 4th Street, Lubbock, TX 79430-0001. E-mail: phrpjs{at}ttuhsc.edu

  • This work was supported by National Institutes of Health Grant AA11643 and the Texas Advanced Research Program under Grant 010674–011.

  • The American Society for Pharmacology and Experimental Therapeutics
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Molecular Pharmacology: 58 (6)
Molecular Pharmacology
Vol. 58, Issue 6
1 Dec 2000
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Research ArticleArticle

Differential Fibronectin Expression in Activated C6 Glial Cells Treated with Ethanol

Li Q. Ren, Daniel K. Garrett, Marie Syapin and Peter J. Syapin
Molecular Pharmacology December 1, 2000, 58 (6) 1303-1309; DOI: https://doi.org/10.1124/mol.58.6.1303

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Research ArticleArticle

Differential Fibronectin Expression in Activated C6 Glial Cells Treated with Ethanol

Li Q. Ren, Daniel K. Garrett, Marie Syapin and Peter J. Syapin
Molecular Pharmacology December 1, 2000, 58 (6) 1303-1309; DOI: https://doi.org/10.1124/mol.58.6.1303
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