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Research ArticleArticle

A3 Adenosine Receptor Activation Triggers Phosphorylation of Protein Kinase B and Protects Rat Basophilic Leukemia 2H3 Mast Cells from Apoptosis

Zhenhai Gao, Bing-Sheng Li, Yuan-Ji Day and Joel Linden
Molecular Pharmacology January 2001, 59 (1) 76-82; DOI: https://doi.org/10.1124/mol.59.1.76
Zhenhai Gao
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Bing-Sheng Li
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Yuan-Ji Day
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Joel Linden
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Abstract

Adenosine accumulates to high levels in inflamed or ischemic tissues and activates A3 adenosine receptors (ARs) on mast cells to trigger degranulation. Here we show that stimulation of rat basophilic leukemia (RBL)-2H3 mast-like cells with the A3 AR agonistsN 6-(3-iodo)benzyl-5′-N-methylcarboxamidodoadenosine (IB-MECA; 10 nM) or inosine (10 μM) stimulates phosphorylation of protein kinase B (Akt). IB-MECA (1 μM) also causes a >50% reduction in apoptosis caused by exposure of RBL-2H3 cells to UV light. Akt phosphorylation is not stimulated by 100 nMN 6-cyclopentyladenosine (A1-selective) or CGS21680 (A2A-selective) and is absent in cells pretreated with wortmannin or pertussis toxin. TheK I values of the AR antagonists BW-1433 and 8-sulfophenyltheophylline (8-SPT) were determined in radioligand binding assays for all four subtypes of rat ARs: BW-1433 (A1, 5.8 ± 1.0 nM; A2A, 240 ± 37; A2B, 30 ± 10; A3, 12,300 ± 3,700); 8-SPT (A1, 3.2 ± 1.2 μM; A2A, 57 ± 4; A2B, 2.2 ± 0.8; A3, >100). BW-1433 and the A3-slective antagonist MRS1523 (5 μM), but not 8-SPT (100 μM), block IB-MECA-induced protection from apoptosis, confirming the A3 AR as the mediator of the antiapoptotic response. The data suggest that adenosine and inosine activate Gi-coupled A3 ARs to protect mast cells from apoptosis by a pathway involving the βγ subunits of Gi, phosphatidylinositol 3-kinase β, and Akt. We speculate that activation of A3ARs on mast cells or other cells that express A3 ARs (e.g., eosinophils) may facilitate their survival and accumulation in inflamed tissues.

Footnotes

    • Received May 17, 2000.
    • Accepted May 10, 2000.
  • Send reprint requests to: Joel Linden, Ph.D., Box MR4 6012 Health Sciences Center, University of Virginia, Charlottesville, Virginia 22908-0466. E-mail: jlinden{at}virginia.edu

  • ↵1 Current address: CV Therapeutics, Palo Alto, California.

  • This work was supported by Grant R01-HL37942 from the National Institutes of Health.

  • The American Society for Pharmacology and Experimental Therapeutics
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Molecular Pharmacology: 59 (1)
Molecular Pharmacology
Vol. 59, Issue 1
1 Jan 2001
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Research ArticleArticle

A3 Adenosine Receptor Activation Triggers Phosphorylation of Protein Kinase B and Protects Rat Basophilic Leukemia 2H3 Mast Cells from Apoptosis

Zhenhai Gao, Bing-Sheng Li, Yuan-Ji Day and Joel Linden
Molecular Pharmacology January 1, 2001, 59 (1) 76-82; DOI: https://doi.org/10.1124/mol.59.1.76

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Research ArticleArticle

A3 Adenosine Receptor Activation Triggers Phosphorylation of Protein Kinase B and Protects Rat Basophilic Leukemia 2H3 Mast Cells from Apoptosis

Zhenhai Gao, Bing-Sheng Li, Yuan-Ji Day and Joel Linden
Molecular Pharmacology January 1, 2001, 59 (1) 76-82; DOI: https://doi.org/10.1124/mol.59.1.76
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