Figure 2
Lipid specificity for modulation of ATP and glibenclamide inhibition. A, effect of 50 μM PC (n = 3), 50 μM PI (n = 6), 50 μM DOG (n = 3), and 10 μM PI(4)P (n = 5) on ATP and glibenclamide sensitivity. Lipids were applied for 45 s and subsequently inhibition by 100 μM ATP and 10 μM glibenclamide was plotted as columns; mean ± S.E.M.; control (contr.) represents inhibition before lipid application (n = 16). B, structure of PI(4,5)P2 and DOGS-NTA. C, change in inhibition by ATP and glibenclamide upon application of PI(4)P (10 μM), PI(3,4)P2 (10 μM), PI(3,4,5,)P3 (10 μM), and DOGS-NTA (10 μM) obtained from experiments as in D plotted as rel. currents as described in Fig.1D. D, currents from a patch exposed to 100 μM ATP, 10 μM glibenclamide, 10 μM DOGS-NTA, and 100 μg/ml polylysine (poly-K) as indicated.