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Molecular Pharmacology

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Research ArticleArticle

Transcriptional Modulation of Mouse μ-Opioid Receptor Distal Promoter Activity by Sox18

Hee-Jeong Im, Dmitri Smirnov, Tomoaki Yuhi, Shalini Raghavan, Jane E. Olsson, George E. Muscat, Peter Koopman and Horace H. Loh
Molecular Pharmacology June 2001, 59 (6) 1486-1496; DOI: https://doi.org/10.1124/mol.59.6.1486
Hee-Jeong Im
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Dmitri Smirnov
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Tomoaki Yuhi
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Shalini Raghavan
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Jane E. Olsson
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George E. Muscat
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Peter Koopman
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Horace H. Loh
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Abstract

Previously, we reported the presence of dual promoters, referred to as distal (DP) and proximal, with a negative regulatory element between them in the mouse μ-opioid receptor (mor) gene. Here we have identified a positive regulatory element influencingmor DP transcription, which contains multiple consensus binding motifs for Sox factors (sex-determining Sry-like high mobility group box-containing genes). In gel supershift assays, the Sox family member Sox18 bound directly to the multiple Sox consensus binding motifs of the mor DP enhancer. Overexpression of Sox18 cDNA increased luciferase activity regulated by the morDP, and did so in a Sox18 concentration-dependent manner. In contrast, overexpression of another Sox member, Sox5, triggered no suchtrans-activation of mor DP-driven luciferase activity or DNA-protein binding activity. These results suggest that Sox18 directly and specifically stimulatesmor gene expression, by trans-activating the mor DP enhancer.

Footnotes

    • Received January 2, 2000.
    • Accepted February 28, 2001.
  • Send reprint requests to: Hee-Jeong Im, Department of Pharmacology, University of Minnesota Medical School, 6-120 Jackson Hall, 321 Church St. S.E., Minneapolis, MN 55455. E-mail:imh{at}tc.umn.edu

  • This research was supported by National Institutes of Health Grants DA-00546, DA-01583, DA-05695, KO5-DA-70554, and the A&F Stark Fund of the Minnesota Medical Foundation.

  • The American Society for Pharmacology and Experimental Therapeutics
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Molecular Pharmacology: 59 (6)
Molecular Pharmacology
Vol. 59, Issue 6
1 Jun 2001
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Research ArticleArticle

Transcriptional Modulation of Mouse μ-Opioid Receptor Distal Promoter Activity by Sox18

Hee-Jeong Im, Dmitri Smirnov, Tomoaki Yuhi, Shalini Raghavan, Jane E. Olsson, George E. Muscat, Peter Koopman and Horace H. Loh
Molecular Pharmacology June 1, 2001, 59 (6) 1486-1496; DOI: https://doi.org/10.1124/mol.59.6.1486

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Research ArticleArticle

Transcriptional Modulation of Mouse μ-Opioid Receptor Distal Promoter Activity by Sox18

Hee-Jeong Im, Dmitri Smirnov, Tomoaki Yuhi, Shalini Raghavan, Jane E. Olsson, George E. Muscat, Peter Koopman and Horace H. Loh
Molecular Pharmacology June 1, 2001, 59 (6) 1486-1496; DOI: https://doi.org/10.1124/mol.59.6.1486
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