Abstract
The effect of fluoride on the rates of carbamylation and decarbamylation of acetylcholinesterase was studied using dimethylcarbamyl fluoride, dimethylcarbamylcholine, and monomethylcarbamylcholine as carbamylating agents. In all cases concentrations of fluoride between 10-4 M and 10-3 M, which markedly decreased the rate of hydrolysis of acetylcholine, also markedly decreased the rate of carbamylation. On the other hand, concentrations of fluoride as high as 0.1 M failed to affect the rate of decarbamylation of the dimethylcarbamyl-enzyme and the monomethylcarbamyl-enzyme. Fluoride was also without effect on the nucleophilic decarbamylation or reactivation caused by hydroxylamine and choline.
- Copyright ©, 1970, by Academic Press Inc.
MolPharm articles become freely available 12 months after publication, and remain freely available for 5 years.Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page.
|
Log in using your username and password
Purchase access
You may purchase access to this article. This will require you to create an account if you don't already have one.