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Molecular Pharmacology

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Research ArticleArticle

Reciprocal Modulation of Voltage-Gated and Background K+ Channels Mediated by Nucleotides and Corticotropin

John J. Enyeart, Lin Xu, Juan Carlos Gomora and Judith A. Enyeart
Molecular Pharmacology July 2001, 60 (1) 114-123; DOI: https://doi.org/10.1124/mol.60.1.114
John J. Enyeart
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Lin Xu
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Juan Carlos Gomora
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Judith A. Enyeart
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Abstract

Bovine adrenal zona fasciculata (AZF) cells express two types of K+-selective ion channels including a rapidly inactivating bKv1.4 current (IA) and an ATP-dependent noninactivating background current (IAC) that sets the resting membrane potential. Whole-cell, patch-clamp recording from cultured AZF cells was used to demonstrate a novel reciprocal modulation of these two K+ channels by intracellular nucleotides and corticotropin. Specifically, increases in IAC activity induced by intracellular ATP, as well as GTP and 5′-adenylyl-imidodiphosphate (AMP-PNP), were accompanied by a corresponding decrease in the amplitude of the voltage-gated IA current. The reduction in IA current was observed only when patch pipettes contained ATP or other nucleotides at concentrations sufficient to support activation of IAC. Conversely, the nearly complete inhibition of IAC by corticotropin was accompanied by the coincident reappearance of functional IA channels. In the absence of IAC current, corticotropin failed to alter IA. The reciprocal modulation of AZF cell K+channels by nucleotides and corticotropin was independent of membrane voltage. These results demonstrate a new form of channel modulation in which the activity of two different K+ channels is reciprocally modulated in tandem through hormonal and metabolic signaling pathways. They further suggest that IA and IAC K+ channels may be functionally coupled in a dynamic equilibrium driven by intracellular ATP and G-protein-coupled receptors. This may represent a unique mechanism for transducing biochemical signals to ionic events involved in cortisol secretion.

  • Abbreviations

    IA
    rapidly inactivating bKv1.4 current in bovine adrenal fasciculata cells
    IAC
    ATP-activated, noninactivating potassium current in bovine adrenal fasciculata cells
    AZF
    bovine adrenal zona fasciculata
    AMP-PNP
    5′-adenylyl-imidodiphosphate
    BAPTA
    1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid
    DPBP
    diphenylbutylpiperidine
    ı̂
    the unitary current
    N
    the number of active channels in any given patch
    Po
    channel open probability
    To
    initial time of recording
    TMAX
    time after IAC K+ current reaches a stable maximum amplitude
    I-V
    current-voltage
    • Received October 27, 2000.
    • Accepted March 14, 2001.
    • The American Society for Pharmacology and Experimental Therapeutics
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    Molecular Pharmacology: 60 (1)
    Molecular Pharmacology
    Vol. 60, Issue 1
    1 Jul 2001
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    Research ArticleArticle

    Reciprocal Modulation of Voltage-Gated and Background K+ Channels Mediated by Nucleotides and Corticotropin

    John J. Enyeart, Lin Xu, Juan Carlos Gomora and Judith A. Enyeart
    Molecular Pharmacology July 1, 2001, 60 (1) 114-123; DOI: https://doi.org/10.1124/mol.60.1.114

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    Research ArticleArticle

    Reciprocal Modulation of Voltage-Gated and Background K+ Channels Mediated by Nucleotides and Corticotropin

    John J. Enyeart, Lin Xu, Juan Carlos Gomora and Judith A. Enyeart
    Molecular Pharmacology July 1, 2001, 60 (1) 114-123; DOI: https://doi.org/10.1124/mol.60.1.114
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