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Molecular Pharmacology

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Research ArticleArticle

The Effects of Isoflurane on Acetylcholine Receptor Channels: 3. Effects of Conservative Polar-to-Nonpolar Mutations within the Channel Pore

Ingobert Wenningmann, Martin Barann, Ana Maria Vidal and James P. Dilger
Molecular Pharmacology September 2001, 60 (3) 584-594;
Ingobert Wenningmann
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Martin Barann
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Ana Maria Vidal
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James P. Dilger
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Abstract

We performed macroscopic and single-channel current measurements on wild-type (WT) and two mutant muscle-type nicotinic acetylcholine (ACh) receptor channels transiently expressed in HEK-293 cells. The mutants contained polar-to-nonpolar substitutions at the 10′ (α2S10′AβT10′Aγδ) and 6′ positions (α2S6′AβγδS6′A) in the M2 pore region of the channel. We studied the behavior of these channels in the absence and presence of the volatile general anesthetic isoflurane. Both mutations changed the gating behavior of the channel. A comparison of the α2S10′AβT10′Aγδ mutant to WT receptors revealed faster desensitization kinetics, increased sensitivity to ACh, a higher efficacy for activation by the partial nicotinic agonist decamethonium, and a greater number of openings per burst. A comparison of the α2S6′AβγδS6′A mutant to WT receptors also revealed increased sensitivity to ACh and an increased burst duration at the single-channel level with ACh as agonist. The α2S10′AβT10′Aγδ mutation increased the sensitivity of the ACh receptor to isoflurane, whereas the α2S6′AβγδS6′A mutation did not. These changes were probably not caused by the differential effects of the mutation on channel gating and desensitization. The increased sensitivity of the α2S10′AβT10′Aγδ receptor to isoflurane is state-dependent; the mutation changes the affinity of the closed state but not that of the open state of the channel.

Footnotes

  • ↵1 A rapid decay is not obvious when inhibition is low in the −/+ application mode with the 10′ mutant. It becomes apparent at higher concentrations of isoflurane.

  • This research was supported in part by Grant GM 42095 from the National Institute of General Medical Sciences, the Department of Anesthesiology, State University of New York, Stony Brook, NY, and the Klinik für Anästhesiologie, Universität Bonn, Germany.

  • I.W. and M.B. contributed equally to this work.

  • Abbreviations:
    ACh
    acetylcholine
    AchR
    acetylcholine receptor
    WT
    wild-type
    QX-222
    N′-(trimethyl-amino-methyl)-2′,6′-xylidide
    ECS
    extracellular solution
    10′αβ
    α2S10′AβT10′Aγδ
    6′αδ
    α2S6′AβγδS6′A
    • Received January 9, 2001.
    • Accepted May 25, 2001.
  • The American Society for Pharmacology and Experimental Therapeutics
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Molecular Pharmacology: 60 (3)
Molecular Pharmacology
Vol. 60, Issue 3
1 Sep 2001
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Research ArticleArticle

The Effects of Isoflurane on Acetylcholine Receptor Channels: 3. Effects of Conservative Polar-to-Nonpolar Mutations within the Channel Pore

Ingobert Wenningmann, Martin Barann, Ana Maria Vidal and James P. Dilger
Molecular Pharmacology September 1, 2001, 60 (3) 584-594;

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Research ArticleArticle

The Effects of Isoflurane on Acetylcholine Receptor Channels: 3. Effects of Conservative Polar-to-Nonpolar Mutations within the Channel Pore

Ingobert Wenningmann, Martin Barann, Ana Maria Vidal and James P. Dilger
Molecular Pharmacology September 1, 2001, 60 (3) 584-594;
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