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Research ArticleArticle

Regulation of the Rat Glutathione S-Transferase A2 Gene by Glucocorticoids: Involvement of Both the Glucocorticoid and Pregnane X Receptors

K. C. Falkner, J. A. Pinaire, G.-H. Xiao, T. E. Geoghegan and R. A. Prough
Molecular Pharmacology September 2001, 60 (3) 611-619;
K. C. Falkner
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J. A. Pinaire
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G.-H. Xiao
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T. E. Geoghegan
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R. A. Prough
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Abstract

Glucocorticoids regulate the rat glutathioneS-transferase A2 (GSTA2) gene in a biphasic manner in cultured hepatocytes that repress gene expression at low concentration (10–100 nM) but induce gene expression at high concentration (>1 μM). High concentrations of the glucocorticoid receptor (GR) antagonist RU38486 (5–10 μM) also induced the expression of GSTA2. These effects were reproduced in HepG2 cells transfected with a luciferase reporter containing 1.6 kilobase pairs of 5′-flanking sequence of GSTA2 and expression plasmids for either GR, pregnane X receptor (PXR) or a combination of both. Dexamethasone t-butylacetate (1 μM t-Bu-DEX) repressed gene expression between 60 to 75% when only GR was expressed. When PXR was expressed, both basal andt-Bu-DEX-dependent gene expression was increased over 2-fold, respectively. Biphasic regulation of gene expression was observed over a broad range of t-Bu-DEX concentrations when expression plasmids for both receptors were cotransfected. Other steroids of the pregnane class induced GSTA2 expression as expected for a PXR-dependent process. Because no canonical responsive element for the PXR-RXRα heterodimer was observed in the 5′-flanking region of the construct, deletion analysis was used to identify a pregnane responsive region between base pairs −700 and −683; this 20-bp region contains the antioxidant response element (ARE). When the ARE sequence was mutated, basal,t-butylhydroquinone- and 17α-hydroxypregnenolone-inducible expression were all lost. These results suggest that PXR interacts with factors binding to the ARE to elicit the pregnane inductive response for GSTA2.

The glutathione S-transferase A2 (GSTA2) gene encodes a protein that is a member of a superfamily of dimeric enzymes of subunit molecular mass between 20 and 30 kDa (Ketterer 1986;Mannervik and Danielson, 1988; Hayes and Pulford, 1995) These proteins play a major role in the detoxification of xenobiotic chemicals by catalyzing the conjugation of glutathione with the electrophilic centers of these toxicants, thus preventing protein or nucleic acid alkylation reactions. In addition, GSTA2 serves as an intracellular binding protein for nonsubstrate ligands, which includes steroids of the pregnane class (Listowsky, 1993) and bile acids including lithocholic acid (Takikawa and Kaplowitz, 1988).

The transcriptional activation of the rat GSTA2 subunit gene by xenobiotic compounds has been well characterized by Pickett and coworkers (Rushmore et al., 1990; Rushmore et al., 1991; Nguyen and Pickett, 1992). Two functionally independent responsive elements, theAh-receptor response element (AhRE, Rushmore et al., 1990) and the antioxidant response element (ARE, Rushmore et al., 1991), are required for the induction of this protein subunit by xenobiotic compounds. Polycyclic and polychlorinated aromatic compounds induce gene expression via the Ah receptor, which binds to the AhRE (Whitlock et al., 1996). The metabolites of aromatic compounds, phenolic antioxidants, phorbol esters, and hydrogen peroxide cause transcriptional activation, apparently through action of the transcription factor Nrf-2 and its heterodimerization partner, a member of the small maf family, which binds to the ARE (Itoh et al., 1999).

Glucocorticoids have been shown to modulate transcription of genes by several different mechanisms. Classically, glucocorticoids act as ligands for the glucocorticoid receptor (GR) to either enhance or repress expression of target genes (Beato, 1989). The simplest interaction of this transcription factor is through binding to a canonical sequence, TGTYCT, often found as an imperfect palindrome separated by three base pairs. This interaction is characterized by a concentration dependence for the synthetic glucocorticoid DEX similar to the known Kd value of GR (less than 10−7 M) and by inhibition of its action by the GR antagonist RU38486.

Kliewer et al. (1998) described another member of the nuclear receptor superfamily, the pregnane X receptor (PXR), that is activated by steroids of the pregnane class and cholestatic bile acids, such as lithocholic acid and 3 keto-lithocholic acid (Staudinger et al., 2001). In addition to these endogenous ligands, this receptor is activated by xenobiotic compounds such as imidazole derivatives and macrolide antibiotics (e.g., rifampicin). In contrast to GR, PXR binds DEX with much lower affinity and the responses to DEX are observed at concentrations greater than 1 × 10−7 M. The ligand dependence is also different from the classical glucocorticoid response; for example, RU38486 serves as an agonist for PXR, rather than an antagonist of GR. PXR binds DNA as an obligate heterodimer with RXRα. In rodents, this heterodimeric complex preferentially recognizes a direct repeat of the nonsteroidal nuclear receptor half-site separated by a three-nucleotide spacer (Kliewer et al., 1998). A DR3 binding sequence of AGTTCAnnnAGTTCA as observed in rat CYP3A23 (Huss et al., 1998; Kliewer et al., 1998;Quattrochi et al., 1998) binds the PXR and is thought to account for the “nonclassical” glucocorticoid induction of rat CYP3A23 protein described by Guzelian and coworkers (Schuetz et al., 1984; Schuetz and Guzelian, 1984; Kocarek et al., 1994).

Our previous work revealed inconsistencies in the effects of glucocorticoids on the expression of GSTA2 in different systems. In isolated rat hepatocytes, DEX at 1 × 10−6 M induced GSTA2 expression (Xiao et al., 1995). However, transient transfection experiments with HepG2 cells, expression of a reporter construct containing 1.62 kb of 5′ flanking region of rat GSTA2 was suppressed by DEX at 1 × 10−5 M (Falkner et al., 1998). The current report is based on the hypothesis that low concentrations of DEX suppress GSTA2 gene expression via a GR-dependent mechanism, whereas high concentrations of DEX induce expression via a PXR-dependent mechanism. This report describes the biphasic concentration-dependence of the effects of DEX on the expression ofGSTA2 in primary adult rat hepatocytes. Furthermore, each phase of this response is reproduced in transient transfection experiments in HepG2 cells by cotransfection of expression plasmids for either GR or PXR.

Experimental Procedures

Materials.

pCMV-β was obtained from Stratagene (La Jolla, CA). The reporter construct p2XDEX-LUC and pRSVGR, the expression vector for the human GR, were a kind gift from Michael Mathis (LSU Medical Center, Shreveport, LA). The expression vector pPXR for the mouse PXR was generously provided by Steven Kliewer (Glaxo Wellcome Research and Development, Research Triangle Park, NC). The expression vector for human RXRα was generously provided by Dan Noonan (University of Kentucky, Lexington, KY).

Reagents for culturing E. coli were obtained from Difco Laboratories (Detroit, MI). Antibiotic/antimycotic solution, nonessential amino acids and Dulbecco's modified Eagle's medium (high modified) were purchased from Mediatech, Inc (Herdon, VA). Fetal bovine serum was obtained from Harlan Bioproducts for Science, Inc. (Indianapolis, IN). DNA purification kits from QIAGEN (Chatworth, CA) were used to produce transfection quality plasmid DNA. Oligonucleotides were purchased from Operon Technologies, Inc. (Alameda, CA). PCR reagents were purchased from Fisher Scientific (Pittsburgh, PA).

Steroid ligands for PXR [i.e., corticosterone, DEX, hydrocortisone, progesterone, 17α-hydroxypregnenolone, and 6α-methyl-17α-hydroxy progesterone acetate (Depo-Provera)] were purchased from the Sigma Chemical Co. (St. Louis, MO). RU38486 was obtained from Roussel Uclaf (Romainville, FRANCE). Pregnenolone was obtained from Mann Research Laboratories, (New York, NY). t-Bu-DEX was purchased from Research Plus, Inc. (Bayonne, NJ). Chlorophenol red-β-d-galactopyranoside was purchased from Roche Molecular Biochemicals (Indianapolis, IN). All other reagents were purchased from commercial suppliers and were either American Chemical Society or molecular biology grade.

Primary Hepatocyte Cell Culture

Hepatocytes were routinely prepared from male adult Sprague-Dawley rats (180–250 g; [Hsd:Sprague-Dawley SD] from Harlan Sprague-Dawley, Indianapolis, IN) by in situ liver collagenase perfusion as described previously (Xiao et al., 1995). Cell viability (>85–90%) was determined by trypan blue exclusion. Hepatocytes were suspended (approx.1 × 106cells/ml) in arginine-free Eagle's minimum essential medium supplemented with l-ornithine and insulin-transferrin-sodium selenite solution. Cells (3 ml) were seeded on 60-mm tissue culture dishes that had been precoated with Matrigel. Once plated, the cells were maintained at 37°C in a humidified atmosphere of 95% air/5% CO2. The media were changed 2 and 24 h after plating. At 24 h, inducing agents, either DEX or RU38486, were added in DMSO. Control cells received equivalent amounts of solvent alone. At the desired time, media were removed from the dishes by aspiration, the cells were washed twice with Dulbecco's phosphate-buffered saline, and mRNA were isolated as described previously (Xiao et al., 1995).

Analysis of mRNA Levels.

Total RNA was isolated by modification of the method of Chomczynski and Sacchi (1987). Northern blot experiments were performed after size-fractionation of the denatured RNA (25 μg) on formaldehyde-containing 1% agarose gels and transfer of the RNAs to Zetaprobe membranes (Life Science Research, Hercules, CA) by capillary transfer. GSTA2 mRNA was detected using a 290-bp fragment of the plasmid pGTB38 kindly provided by Cecil Pickett (Schering Plough, Kenilworth, NJ). Cytochrome P450 3A1 mRNA was detected using a synthetic oligonucleotide termed ON-1 by Waxman et al. (1992) [5′-ACCACATTTCTGACGAATTCAGCAGAACTC-3′]. This probe has a single nucleotide mismatch with the sequence reported forCYP3A23, the major DEX-inducible cytochrome P450 in the rat (Huss et al., 1998). CYP2B1/2 mRNA was detected using a 1.7-kbEcoRI fragment of the plasmid pSP450, which contains the entire coding sequence for CYP2B1 obtained from Milton Adesnick (New York University Medical Center, New York, NY). These nucleic acid probes were labeled with [32P]dCTP using the random primer labeling procedure. Hybridization was carried out at 43°C overnight in 0.25 M sodium phosphate buffer, pH 7.2. As a control, β-actin mRNA levels were measured using a mouse cytoskeletal β-actin (Xiao et al., 1995).

Plasmid Constructs.

Luciferase constructs containing either 0.164 or 1.651 kb of the 5′-flanking region, p0.164YaLUC and p1.62YaLUC, respectively, of the rat GSTA2 have been described previously (Falkner et al., 1998). Deletion constructs where produced by either sequential subcloning of PCR products or introduction of ds-oligonucleotides into p0.164YaLUC. To prepare a reporter gene with the GSTA2 promoter containing a polycloning site, primers BMYaUNI (5′-AGACT-AAGCTTGGGTTGTAAAAGAGATACTGA) and 164BGLTOP (5′-AGACT-AGATCT-CTTGTCAGCCCCTCCCCCCA-3′) were used with p1.6YaCAT as a template to produce a 252-bp fragment containing 164 bp of the 5′-flanking region of the GSTA2 gene. PCR was performed in a Thermolyne Amplitron II thermal cycler (Barnstead/Thermolyne, Dubuque, IA) with 2 mM Mg2+. PCR products were generated through 20 cycles of the following steps: denaturing temperature, 94°C for 0.5 min; annealing temperature, 50°C for 1 min; and elongation temperature, 72°C for 1 min. The PCR fragments were initially subcloned into pCR2.1 vector (Invitrogen, Carlsbad, CA) and using the BglII and HindIII restriction sites, the recovered PCR fragment was subcloned into the pGL2-basic vector (Promega, Madison, WI) to form p0.164bYaLUC. Other fragments of the 5′-flanking region were generated using the primers listed in Table 1, which were subcloned into the pCR2.1 recovery vector. These fragments were subcloned into p0.164bYaLUC using either restriction sites in the 5′-flanking regions of the primers or unique restriction sites in the polycloning region of pCR2.1. No differences have been observed in the function of either p0.164YaLUC or p0.164bYaLUC.

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Table 1

Oligonucleotides used for PCR to make fragments of the GSTA2promoter

The pARE-LUC plasmid containing the sequences from −722 to −682 bp was constucted in a method identical with p0.164YaLUC (Falkner et al., 1998), except that ARE-CAT (Rushmore et al., 1991) was used as a template rather than 0.164YaCAT. The construct −703 to −683 was produced by annealing oligonucleotides OKF82 (5′-TAATGGTGACAAAGCAACTT-3′) and OKF83 (5′-TAAAGTTGCTTTGTCACCAT-3′) and subcloning a single copy of the ds-oligonucleotide into theNdeI site of p0.164YaLUC. Likewise, the −722 to −682 ARE mutant construct was produced by subcloning a single copy of the annealed oligonucleotides OKF 80 (5′-TAAAGTTGCTTTGTCCCCATTAGCAATGCCATTTCCAAGCTC-3′) and OKF81(5′-TAGA-GCTTGGAAATGGCATTGCTAATGGGGACAAAGCAACTT-3′) into theNdeI site of p0.164YaLUC.

Cells and Culture Conditions.

The human hepatoblastoma cell line HepG2 was obtained from the American Type Culture Collection (Manassas, VA). Cells were maintained in Dulbecco's modified Eagle's medium supplemented with antibiotic/antimycotics, nonessential amino acids and 10% fetal bovine serum. The hepatoma cells were incubated at 37°C in a 5% carbon dioxide atmosphere and were subcultured every 2 to 3 days.

Transfection of Hepatoblastoma Cells.

HepG2 cells were plated into 12-well tissue culture plates and transfected using the calcium phosphate method of Gorman et al. (1982) at 40% cell confluence. DNA-CaCl2-HEPES buffered precipitates were added to the cells and, after 24 h, the media were changed. The cells were subsequently treated with various agents as 500× concentrated stocks in DMSO; controls received an equivalent volume of DMSO alone. After an additional 24 h, the cell protein was harvested with 100 μl of cell lysis buffer (Promega Corp., Madison, WI) according to manufacturer's instructions and subjected to a single freeze/thawing event. All cells were cotransfected with 500 ng of pCMV-β as a transfection control. Routinely, 125 ng of receptor expression plasmids (pPXR or pRSVGR) and 1 μg of the variousGSTA2 reporter constructs, including p1.62YaLUC, were added to each well.

Assays of β-Galactosidase and Luciferase Activity.

Luciferase activity of cell extracts was determined using the luciferase assay system from Promega (Madison, WI) in a Berthold Model LB9501 Lumat luminometer (Wallac, Inc., Gaithersburg, MD). For the β-galactosidase assays, cell extracts were incubated with chlorophenol red β-galactopyranoside at 37°C for 30 min. Activity was determined spectrophotometrically at 574 nm with a μQuant microplate spectrophotometer (Biotek Instruments Inc., Winooski, VT).

Statistical Analysis.

The data were analyzed using a Student's t test.

Results

Varying Concentrations of Glucocorticoids Cause Biphasic Changes inGSTA2 Expression in Cultured Rat Hepatocytes.

The glucocorticoid DEX decreases expression of the GSTA2 mRNA in cultured rat hepatocytes at concentrations up to 1 × 10−7 M (Fig. 1, A and B). However, at concentrations above 1 × 10−6 M, an increase in mRNA levels was observed, demonstrating two phases of response to DEX for the GSTA2gene. Because induction occurred with a concentration-dependence consistent with a PXR-dependent process, we tested the effects of DEX on the expression of CYP3A23 and CYP2B1/2 genes as shown in Fig. 1C. The effects of glucocorticoids between 1 × 10−11 and 1 × 10−7M for CYP2B1/2 expression was similar to that observed withGSTA2 expression. Therefore, within the concentration range associated with activation of the GR (up to 1 × 10−7 M), DEX exerts a concentration-dependent repression of both GSTA2 and CYP2B1/2 message levels. In our hands, basal expression of CYP3A23 in cultured rat hepatocytes cultured for 24 to 48 h was very low.

Figure 1
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Figure 1

Effect of DEX on levels of mRNAs specific forGSTA2, CYP3A23, andCYP2B1/2 in cultured rat hepatocytes. Total RNA was isolated from cultured hepatocytes treated 72 h with either vehicle (DMSO) or varying concentrations of DEX. A and B, Northern analysis of GSTA2 and β-actin mRNAs was performed as described under Experimental Procedures. Absorbance of relevant bands on the autoradiograms were measured using a Bio-Rad model 620 videodensitometer and normalized with the absorbance values obtained from the autoradiogram of the loading control, β-actin. The data shown are representative of three separate experiments summarized in Table 2. C, Northern analysis was performed after hybridization of total mRNA to 32P-labeled cDNA probes for CYP2B1/2 and β-actin or ds-oligonucleotide probe specific for CYP3A23. The data shown are representative of two to three separate experiments and is summarized in Table 2.

At concentrations above 1 × 10−6 M, a 2- to 3-fold increase in mRNA levels was observed for GSTA2 andCYP2B1/2 (Fig. 1, B and C), whereas a 10- to 11-fold increase was observed in the expression of the CYP3A23 gene. In addition, a 2- to 3-fold induction of GSTA2 protein and enzyme activity was observed upon treatment of primary adult rat hepatocytes with concentrations of DEX at or above 1 × 10−6 M (data not shown). There was a striking similarity of both the shape and magnitude of the concentration-response curves for GSTA2 andCYP2B1/2. These results confirm those published by Kocarek et al. (1994) for the expression of CYP2B1/2 andCYP3A23. The results presented show that glucocorticoids such as DEX are capable of regulating the expression of the rat

GSTA2 gene in a biphasic manner and are consistent with our hypothesis that low concentrations of DEX suppress GSTA2gene expression via a GR-dependent mechanism, whereas high concentrations of DEX induce expression via a PXR-dependent mechanism. These results also resolve the apparent conflict between our earlier published work (Xiao et al., 1995; Falkner et al., 1998). The data shown in Fig. 1 are reproducible and a statistical analysis of the induction of GSTA2, CYP3A23, and CYP2B1/2 mRNA levels are shown in Table 2.

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Table 2

Statistical analyses of Northern blots of GSTA2,CYP3A23, and CYP2B1/2 mRNA levels in rat hepatocytes treated with DEX or RU38486 for 72 hours in culture

The GR Antagonist RU38486 Induces GSTA2 mRNA Levels in Cultured Rat Hepatocytes.

Fig. 2 shows the concentration-dependent effects of RU38486 on GSTA2 mRNA. GSTA2 mRNA is induced approximately 3-fold by treatment of the hepatocytes with high concentrations (10 μM) of RU38486. Although RU38486 is a potent antagonist of GR at 5–10 μM, this compound is an agonist for the PXR (Kliewer et al., 1998) and, at these concentrations, has been shown to induce both CYP3A23 and CYP2B1/2 (Kocarek et al., 1994). Thus, the ability of RU38486 to serve as an agonist for GSTA2 induction correlates well with its known function as a PXR ligand. The results shown in Fig. 2 for GSTA2are nearly identical with the changes in CYP2B1/2 mRNA when primary adult rat hepatocytes were treated with RU38486 (Kocarek et al., 1994). The data shown in Fig. 2 are reproducible and a statistical analysis of the effect of RU38486 on GSTA2 mRNA is shown in Table 2. The similarity of these concentration-response curves suggests that DEX and RU38486 regulate GSTA2 and CYP2B1/2 through two different mechanisms, probably due to action of GR and PXR.

Figure 2
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Figure 2

Effects of the GR antagonist RU38486 on levels of mRNAs specific for GSTA2 in cultured rat hepatocytes. Total RNA was isolated from cultured hepatocytes treated 72 h with either vehicle (DMSO) or varying concentrations of RU38486. Northern analysis of the mRNAs was performed as described underExperimental Procedures. Absorbance of relevant bands on the autoradiograms were measured and normalized to the Absorbance values of the loading control, β-actin. The data shown are representative of three separate experiments and is summarized in Table2.

Cotransfection of GR and PXR Accounts for the Biphasic Regulation of a GSTA2 Reporter Construct byt-Bu-DEX.

To test the hypothesis that the biphasic regulation of GSTA2 was mediated by both a classical GR-dependent mechanism and a nonclassical (PXR-dependent) mechanism, experiments were carried out in HepG2 cells transiently transfected with various plasmids. This system allows one to manipulate expression of individual receptors and to reproduce the results seen in primary cultures of rat hepatocytes. HepG2 cells were transiently transfected with p1.62YaLUC, a luciferase reporter gene containing 1.62 kb of the 5′-flanking region of GSTA2, and expression vectors for either the human GR and/or murine PXR in the absence or presence of 1 × 10−5, 1 × 10−7, and 1 × 10−10M t-Bu-DEX. Because the Kd value of GR for DEX is approximately 10 to 50 nM, we expected little effect of the lowest concentration of t-Bu-DEX (1 × 10−10 M) and a maximal glucocorticoid effect at 1 × 10−7 M. Likewise, at 1 × 10−7 M t-Bu-DEX or less, we anticipated little effect on the PXR. However, we anticipated that mPXR would be activated at the highest concentration of t-Bu-DEX used in this experiment, 1 × 10−5 M. As shown in Fig. 3, t-Bu-DEX at any concentration had no effect on the expression of theGSTA2 reporter in the absence of coexpressed receptors. This suggests that the endogenous expression of these receptors is not sufficiently high in HepG2 cells to modulate reporter gene expression in transfection experiments. Consistent with our earlier results (Falkner et al., 1998) when GR was cotransfected with 1.65YaLUC in HepG2 cells and the cells were treated with t-Bu-DEX, negative regulation of this reporter was observed.

Figure 3
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Figure 3

Receptor-dependent effects of t-Bu-DEX on luciferase activity in HepG2 cells transiently transfected with the plasmid p1.62YaLUC. Luciferase and β-galactosidase assays were performed on lysed HepG2 cells that had been transiently transfected with p1.62YaLUC, the control vector pCMV-β and as specified expression vectors for the nuclear receptors, pRSVGR or pPXR as described under Experimental Procedures. Cells were treated with vehicle or varying concentrations oft-Bu-DEX for 24 h. The normalized luciferase activity is expressed as relative light units divided by β-galactosidase activity and is the mean ± S.D. of three wells. *p < 0.05, significantly different from its control; **p < 0.005, significantly different from cells without receptor.

In contrast to the results with GR, there was a doubling of the basal expression of the reporter gene when PXR was coexpressed in HepG2 cells (Fig. 3), possibly reflecting either the presence of PXR ligands in the fetal bovine serum or a role for PXR/RXRα in basal expression. When charcoal treated serum was utilized, PXR still had an effect on basal expression (data not shown). Upon addition of 1 × 10−5 M t-Bu-DEX, there was a further 65% increase in expression; no statistically significant difference in expression was seen at 1 × 10−7 or 1 × 10−10 M t-Bu-DEX. These results demonstrate a role for PXR in t-Bu-DEX-dependent induction of GSTA2 expression.

Intermediate effects were observed when both GR and PXR were cotransfected (Fig. 3). The basal rate was increased, but negative regulation by concentrations of t-Bu-DEX which activate GR was noted. This effect was reversed to control levels of expression with 1 × 10−5 M t-Bu-DEX. Because GR is activated by either t-Bu-DEX (Fig. 3) or DEX (Falkner et al., 1998), the negative effect must be caused byt-Bu-DEX functioning as a GR ligand, and the reversal at 1 × 10−5 M must be caused by its action as a ligand for PXR. These results demonstrate that the biphasic response of GSTA2 gene expression is caused by t-Bu-DEX acting through two independent nuclear receptor-mediated mechanisms.

An extended concentration-response with t-Bu-DEX was performed in this reporter gene system in the presence of either GR alone or GR plus PXR (Fig. 4). As anticipated, when GR alone was cotransfected, treatment witht-Bu-DEX caused a simple monotonic decline in expression of p1.62YaLUC. In the presence of both GR and PXR, more complex biphasic regulation of expression was observed. Although we normalized the basal activity for cells expressing either GR or PXR plus GR, basal expression in the presence of PXR was increased approximately 2-fold. There was negative regulation up to 1 × 10−7 M t-Bu-DEX consistent with this compound mediated its effects acting through the GR. At higher concentrations up to 1 × 10−5 M, a 2- to 2.5-fold induction of expression over basal expression of this gene was observed, again consistent with the involvement of PXR. We observed that 1 × 10−4M t-Bu-DEX was toxic to HepG2 cells as observed by decreases in cell number and viability, which probably accounts for the decline in expression observed at 1 × 10−4 Mt-Bu-DEX.

Figure 4
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Figure 4

Concentration-dependent effects oft-Bu-DEX on luciferase activity in HepG2 cells transiently transfected with the plasmid p1.62YaLUC. Luciferase and β-galactosidase assays were performed on lysed HepG2 cells that had been transiently transfected with p1.62YaLUC, the control vector pCMV-β, and expression vectors for the nuclear receptors, pRSVGR or pPXR as described under Experimental Procedures. Cells were treated with vehicle or varying concentrations oft-Bu-DEX for 24 h. The normalized luciferase activity is expressed as relative light units divided by β-galactosidase activity and is the mean ± S.D. of three wells. Basal expression in the presence of cotransfected PXR was 1.8-fold higher than that seen in the presence of cotransfected GR. *p < 0.01, statistically different from cells not treated with t-Bu-DEX.

Interestingly, in cells cotranfected with PXR and GR, the level of expression of the GSTA2 reporter constructs was greater at the lowest concentration of t-Bu-DEX (1 × 10−10) tested than the control value. Although this response was variable, it was observed both in transient transfection experiments with HepG2 cells and in isolated rat hepatocytes. Several groups (Huss and Kasper, 2000; Pascussi et al., 2000) have reported that the expression several nuclear receptors, including RXRα and PXR, are under glucocorticoid control. Thus, the elevation of basal expression of GSTA2 reporter expression in cells transfected with both PXR and GR may reflect increased expression of RXRα which affects basal expression.

Over-Expression of PXR and RXRα Increases Basal Expression of aGSTA2 Reporter Gene.

Because PXR heterodimerizes with RXRα to bind to its responsive element (Kliewer et al., 1998), we tested the effects of cotransfection of an expression vector for RXRα on expression of p1.62YaLUC. As is shown in Fig.5, cotransfection of a expression vector for RXRα had no effect on luciferase expression on either basal ort-Bu-DEX treated cells. As anticipated, cotransfection of PXR caused an increase in basal level expression of approximately 1.5-fold and a significant increase in expression was observed in cells treated with 1 × 10−5 Mt-Bu-DEX. When both RXRα and PXR were coexpressed, the basal expression rate was markedly increased, butt-Bu-DEX-dependent increases in expression were not observed suggesting that PXR-RXRα apparently interact to increase basal expression of GSTA2 reporter constructs. In cells cotransfected with both receptors, the level of increase of basal expression was greater than the t-Bu-DEX-dependent expression when PXR alone was cotransfected. The loss of ligand-inducibility suggests that corepressors may be limiting when RXRα and PXR are overexpressed.

Figure 5
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Figure 5

Effect of cotransfection of RXRα on the PXR-dependent t-Bu-DEX induction of luciferase activity in HepG2 cells transiently transfected with the plasmid p1.62YaLUC. Luciferase and β-galactosidase assays were performed on lysed HepG2 cells that had been transiently transfected with p1.62YaLUC, the control vector pCMV-β and as specified, expression vector for the nuclear receptors pRXRα or pPXR as described underExperimental Procedures. Cells were treated with vehicle or t-Bu-DEX (10 μM) for 24 h. The normalized luciferase activity is expressed as relative light units divided by β-galactosidase activity and is the mean ± S.D. of three wells. *p <0.09, , significantly different from control cells; **p < 0.03, significantly different from cells expressing PXR.

PXR-Ligands Induce Expression of a GSTA2 Reporter Gene.

To establish which steroids may regulate this gene at concentrations 1 × 10−5 M, we performed the reporter gene expression assay using known activators/ligands for PXR as shown in Fig. 6. The best ligands were the pregnanes pregnenolone (2.5-fold) and 17α-hydroxypregnenolone (2.4-fold). These pregnanes were the best naturally occurring ligands for PXR in the induction of expression of a DR3 reporter in CV-1 cells. The ligands t-Bu-DEX (1.7-fold), RU 38486 (1.6-fold), progesterone (1.5-fold), cortisol (1.5-fold), deoxycortisone (1.4-fold), and 6α-methyl-17α-hydroxy progesterone acetate (1.4-fold) also caused significant induction in enzyme activity. In contrast, DEX (1.1-fold) and hydrocortisone (0.87-fold) did not significantly induce reporter gene expression via PXR. The steroids that caused a significant induction in activity have all been shown to be ligands for the PXR when tested astrans-activators of a DR3 response element-reporter system in transient transfection assays with CV-1 cells (Kliewer et al., 1998). There is, however, a difference in potency between the responses, in that t-Bu-DEX is a much better inducer of DR3-mediated reporters in CV-1 cells than the naturally-occurring pregnanes, 17α-hydroxypregnenolone and pregnenolone, where as the reverse is true for the t-Bu-DEX-dependent regulation ofGSTA2 in HepG2 cells (Fig. 6).

Figure 6
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Figure 6

Effect of treatment with various steroids on the PXR-dependent induction of luciferase activity in HepG2 cells transiently transfected with the plasmid p1.62YaLUC and pPXR. Luciferase and β-galactosidase assays were performed on lysed HepG2 cells that had been transiently transfected with p1.62YaLUC, the control vector pCMV-β and pPXR as described under Experimental Procedures. Cells were treated with vehicle or 10 μM concentration of each steroid for 24 h. The normalized luciferase activity is expressed as relative light units divided by β-galactosidase activity and is the mean ± S.D. of three wells. *p < 0.02; statistically different from control cells.

Deletion Analysis of the 5′-Flanking Region of GSTA2to Define the Location of the PXR-Responsive Element.

Because the 5′-flanking region of the GSTA2 gene does not contain sequences with identity to or high similarity to the canonical DR3 binding sequence for the murine PXR, we undertook a deletion strategy to identify the element through which the PXR-dependent induction process was acting. As seen in Fig. 7, our initial deletion construct that included the area of 5′-flanking region between −914 and −638 bp relative to the transcription start site was responsive to 17α-hydroxypregnenolone-dependent induction. This construct contains the elements that are responsible for induction of this gene by xenobiotic compounds, namely the AhRE and ARE responsive elements. Further deletion analysis identified the region between base pairs −703 and −683 as responsible for the pregnane-dependent induction of the gene. Constructs that did not contain this sequence were not pregnane-responsive. The ARE is located between base −703 and −683; thus, our results show PXR acting within the same 20-bp region containing the ARE.

Figure 7
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Figure 7

Deletion analysis of the 5′-flanking region of theGSTA2 gene. A series of luciferase constructs were prepared with deletions in the 5′-flanking region of theGSTA2 gene and were tested by transient cotransfection with pPXR in HepG2 cells as described under Experimental Procedures. The cells were treated for 24 h. with either dimethyl sulfoxide or 17-hydroxypregnenolone in DMSO. The normalized luciferase activity is expressed as relative light units divided by β-galactosidase activity and is the mean ± S.D. of three wells. *p < 0.03, statistically different from its control.

Mutation of the GSTA2 ARE in a pARE-LUC Reporter Oblates Induction of Expression by PXR Ligands andt-Butylhydroquinone.

To further define the element through which the pregnane effect occurs, we used a plasmid that contains a single base pair mutation in the ARE core sequence (GTGACnnnGC to GGGACnnnGC) that has been previously shown to cause a loss of basal level activation and preventt-butylhydroquinone-dependent induction (Rushmore et al., 1991). As is shown in Fig. 8, botht-butylhydroquinone (an ARE-activating chemical) and 17α-hydroxy-pregnenolone (a PXR-activating chemical) induced expression of this wild-type reporter construct apparently in an additive manner. As expected from the work of Rushmore et al. (1991), the reporter with the mutated ARE had much lower basal activity. The mutated reporter construct was also not responsive to treatment with either 17α-hydroxypregnenolone or t-butylhydroquinone which suggests that the mutated base pair is critical for either inductive process (i.e., activation via either Nrf2 or PXR).

Figure 8
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Figure 8

Effect of mutation of a GSTA2 pARE-LUC reporter gene on induction of luciferase activity by eithert-butylhydroquinone or 17-hydroxypregnenolone. Luciferase and β-galactosidase assays were performed on lysed HepG2 cells that had been transiently transfected with ARE-LUC, the control vector pCMV-β, and the expression vector pPXR as described underExperimental Procedures. Cells were treated for 24 h with vehicle or 50 μM of t-butylhydroquinone or 17α-hydroxypregnenolone either alone or in combination for 24 h. The normalized luciferase activity is expressed as relative light units divided by β-galactosidase activity and is the mean ± S.D. of three wells. *p < 0.07, statistically different from control cells.

Discussion

The results presented show that glucocorticoids such as DEX are capable of regulating the expression of the rat GSTA2 gene in a biphasic manner. These results resolve the apparent conflict between our earlier published work (Xiao et al., 1995) in which glucocorticoids at 1 × 10−6 M induced expression of GSTA2 message in primary rat hepatocytes, whereas identical concentrations of the synthetic corticosteroid repressed expression (Falkner et al., 1998) of reporter constructs transiently transfected into HepG2 cells in a GR-dependent process. Our results show that the regulation of expression in primary hepatocytes is biphasic and that at concentrations of DEX greater than 10−7M gene expression of GSTA2 is induced. However, at lower concentrations of DEX, there is a suppression of gene activation consistent with the involvement of GR. Our studies using transient transfection of reporter plasmids in HepG2 cells showed that neither the negative GR-dependent nor the positive PXR-dependent DEX effects are observed if the respective receptors are not cotransfected. Negative regulation was observed only when GR was cotransfected and likewise, positive regulation was only observed when PXR was cotransfected.

The prototypical model of gene induction at high concentrations of DEX has been well documented for expression of CYP3A23 in the rat. Studies by Guzelian and coworkers (Schuetz et al., 1984; Schuetz and Guzelian, 1984) have shown that this gene is induced by supraphysiological concentrations of glucocorticoids at the transcriptional level. Subsequent studies (Huss et al., 1998;Quattrochi et al., 1998) have shown that the response is mediated through a DR3 element that can bind a PXR/RXRα heterodimer. In addition, other compounds that have been shown to be ligands for the PXR also activate this response element (Kliewer et al., 1998). These compounds include other pregnanes, RU38486, the macrolide antibiotic rifampicin, and imidazole derivatives. Recently, Staudinger et al. (2001) have shown that cholestatic bile acids, such as lithocholic acid and 3-ketolithocholic acid, are excellent ligands for PXR and suggest a role for this receptor in bile acid synthesis and excretion. The ligand-mediated activation of this receptor usually occurs only at high, supraphysiological concentrations of these ligands, consistent with a role for this receptor in detoxication, a reaction catalyzed by CYP3A23.

GSTA2 is a member of a superfamily of proteins that catalyzes the reaction between the sulfhydryl group of the tripeptide glutathione and electronegative centers found in lipophilic molecules (Ketterer, 1986;Mannervik and Danielson, 1988). In addition to its role as an active catalyst, this abundant protein also acts as a binding protein for steroids (Listowsky, 1993) and cholestatic bile acids (Takikawa and Kaplowitz, 1988); as such, it was once named ligandin (Habig et al., 1974). The role as a binding protein may be to facilitate transport of its potentially toxic ligands in the cytosolic compartment, either to specific membrane-spanning transporters or to membrane-associated cytochromes P450 to increase the rate of degradation of these ligands. Thus, the presence of an element capable of supporting a “nonclassical” or PXR-mediated mechanism of induction may be a reflection of the importance of the ligandin role this protein plays.

The transcriptional regulation of GSTA2 by xenobiotic compounds has been intensively studied by Picket and coworkers (Rushmore et al. 1990, 1991; Nguyen and Pickett, 1992). Two elements have been identified, namely the AhRE and the ARE. The work presented in this article indicates that the response element that mediates the PXR-dependent induction of GSTA2 gene expression is located within the ARE, because mutation of the ARE core sequence (GTGACaaaGC to GGGACaaaGC) caused a loss of both t-butylhydroquinone- and PXR-dependent responses. This sequence is required for induction of GSTA2 message and protein by metabolites of aromatic compounds, phenolic antioxidants, phorbol esters, and hydrogen peroxide. The ARE sequence is thought to bind the Cap-N-Collar family transcription factor, Nrf-2 and its obligate heterodimerization partner, a member of the small maf family (Itoh et al., 1999). The mutation of the core sequence above has been shown to result in loss of protein binding (Nguyen and Pickett, 1992).

In contrast, rat QOR gene expression in hepatocytes was negatively regulated in a monotonic manner and this response was blunted by RU38486, suggesting a role for GR alone (J. A. Pinaire and R. A. Prough, unpublished results; Xiao et al., 1995). No induction of the QOR gene products was observed. Since GSTA2and QOR both contain ARE sequences and only GSTA2 expression is affected by high concentrations of DEX in rat hepatocytes, the regulation can not simply be one in which PXR interacts with Nrf-2 to bring about this response. This suggests a novel mechanism for PXR action, namely one in which PXR must interact with another transcription factor.

Interestingly, the tamoxifen-dependent induction of the humanQOR gene expression in MCF-7 cells has also been shown to be mediated by an electrophilic element or ARE (Montano and Katzenellenbogen, 1997; Montano et al., 1998). However, unlike the ARE in the GSTA2 gene, the ARE in the 5′-flanking region of theQOR gene contains a perfect canonical AP-1 site. This effect, which requires ERβ, involves protein-protein interaction with the human prevents mitoticcatastrophe 2 (hPMC2) gene (Montano et al., 2000). At present, it is not known whether the PXR effect observed withGSTA2 is mediated through interaction with the Nrf-2/maf, hPMC2 or another transcription factor. This 20-bp sequence contains neither a nuclear receptor half site nor an AP1 sequence. The data presented here demonstrate that expression of native ratGSTA2 genes in cultured rat hepatocytes is biphasic and suggest that GR and PXR account individually for the 2 phases.

In contrast to the rodent models, relatively little is known about the effects of glucocorticoids or pregnanes on the expression of human glutathione S-transferases. Although GR is highly conserved between rodents and humans, PXR has diverged considerably (Jones et al., 2000). Therefore, we cannot easily predict whether the mechanism presented here is conserved in the human. PXR in humans has different ligand specificity and even a different canonical binding element sequence than the rodent PXRs. The human PXR binds preferentially to an inverted repeat of AGGTCA half-sites separated by six nucleotides (IR6) in contrast to the rodent DR3. Therefore, examination of the species difference in the response, the sequence requirements for the ARE, and the transcription factors involved will be the focus of future studies.

Acknowledgments

We acknowledge Mary Pendleton for her expert technical assistance in the transient transfection experiments and to Milton Adesnick, Steven Kliewer, Michael Mathis, Dan Noonan, and Cecil Pickett for providing us with the expression plasmids used.

Footnotes

  • Supported by National Institutes of Health Grant DK54774 (R.A.P.) and a grant from the University of Louisville School of Medicine (K.C.F.).

  • Abbreviations:
    GSTA2
    glutathione S-transferase A2
    Ah
    aryl hydrocarbon
    AhRE
    Ah-receptor response element
    ARE
    antioxidant response element
    PXR
    pregnane X receptor
    kb
    kilobase pair(s)
    DEX
    dexamethasone
    GR
    glucocorticoid receptor
    PCR
    polymerase chain reaction
    t-Bu-DEX
    dexamethasonet-butylacetate
    RU38486
    17β-hydroxy-11β-(4-dimethylamino-phenyl)-17α-(prop-1-ynyl)-estra-4,9-dien-3-one
    DMSO
    dimethyl sulfoxide
    bp
    base pair(s)
    RXRα
    retinoid X-receptor-α
    DR3
    direct repeat separated by 3 base pairs
    QOR
    NAD(P)H:quinone oxidoreductase
    • Received March 22, 2001.
    • Accepted June 12, 2001.
  • The American Society for Pharmacology and Experimental Therapeutics

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Molecular Pharmacology: 60 (3)
Molecular Pharmacology
Vol. 60, Issue 3
1 Sep 2001
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Regulation of the Rat Glutathione S-Transferase A2 Gene by Glucocorticoids: Involvement of Both the Glucocorticoid and Pregnane X Receptors

K. C. Falkner, J. A. Pinaire, G.-H. Xiao, T. E. Geoghegan and R. A. Prough
Molecular Pharmacology September 1, 2001, 60 (3) 611-619;

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Regulation of the Rat Glutathione S-Transferase A2 Gene by Glucocorticoids: Involvement of Both the Glucocorticoid and Pregnane X Receptors

K. C. Falkner, J. A. Pinaire, G.-H. Xiao, T. E. Geoghegan and R. A. Prough
Molecular Pharmacology September 1, 2001, 60 (3) 611-619;
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