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Molecular Pharmacology

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Research ArticleArticle

The Effect of Thiopurine Methyltransferase Expression on Sensitivity to Thiopurine Drugs

Sally A. Coulthard, Linda A. Hogarth, Margaret Little, Elizabeth C. Matheson, Christopher P. F. Redfern, Lynne Minto and Andrew G. Hall
Molecular Pharmacology July 2002, 62 (1) 102-109; DOI: https://doi.org/10.1124/mol.62.1.102
Sally A. Coulthard
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Linda A. Hogarth
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Margaret Little
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Elizabeth C. Matheson
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Christopher P. F. Redfern
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Lynne Minto
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Andrew G. Hall
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Abstract

Although the thiopurine drugs 6-mercaptopurine (6-MP) and 6-thioguanine (6-TG) are well established agents for the treatment of leukemia, controversies remain regarding their main mode of action. Previous evidence has suggested that although 6-TG exerts a cytotoxic effect through incorporation of 6-thioguanine nucleotides into newly synthesized DNA (DNA-TGN), an important component of the mode of action of 6-MP is inhibition of purine de novo synthesis (PDNS) through the production of S-methyl-thioinosine 5′-monophosphate (MeTIMP), not formed in cells exposed to 6-TG. We have shown that thiopurine methyltransferase (TPMT) modulates this effect. By transfection of the human TPMT gene using an inducible system to produce a 3.8-fold increase in TPMT activity in the ecdysone receptor 293 embryonic kidney cell line, we demonstrated a 4.4-fold increase in sensitivity to 6-MP. This was associated with a rise in intracellular levels of MeTIMP but a decrease in levels of DNA-TGN. In contrast, induction of TPMT produced a 1.6-fold decrease in sensitivity to 6-TG, a decrease in levels of DNA-TGN, and an increase in levels of methylated thioguanosine monophosphate. Exposure of cells to equitoxic doses of drug showed similar incorporation of DNA-TGN for 6-TG but for 6-MP significantly reduced DNA-TGN in TPMT-induced compared with uninduced cells. For equitoxic doses of 6-MP, equivalent levels of MeTIMP correlated with equivalent amounts of PDNS. These observations suggest that intracellular TGN levels do not give an accurate reflection of cytotoxic potential in patients treated with 6-MP, because different levels of DNA-TGN may be associated with equitoxic effects.

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Molecular Pharmacology: 62 (1)
Molecular Pharmacology
Vol. 62, Issue 1
1 Jul 2002
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Research ArticleArticle

The Effect of Thiopurine Methyltransferase Expression on Sensitivity to Thiopurine Drugs

Sally A. Coulthard, Linda A. Hogarth, Margaret Little, Elizabeth C. Matheson, Christopher P. F. Redfern, Lynne Minto and Andrew G. Hall
Molecular Pharmacology July 1, 2002, 62 (1) 102-109; DOI: https://doi.org/10.1124/mol.62.1.102

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Research ArticleArticle

The Effect of Thiopurine Methyltransferase Expression on Sensitivity to Thiopurine Drugs

Sally A. Coulthard, Linda A. Hogarth, Margaret Little, Elizabeth C. Matheson, Christopher P. F. Redfern, Lynne Minto and Andrew G. Hall
Molecular Pharmacology July 1, 2002, 62 (1) 102-109; DOI: https://doi.org/10.1124/mol.62.1.102
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