Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Molecular Pharmacology
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Molecular Pharmacology

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit molpharm on Facebook
  • Follow molpharm on Twitter
  • Follow molpharm on LinkedIn
Research ArticleArticle

DNA Sequence Specificity for Topoisomerase II Poisoning by the Quinoxaline Anticancer Drugs XK469 and CQS

Hanlin Gao, Edith F. Yamasaki, Kenneth K. Chan, Linus L. Shen and Robert M. Snapka
Molecular Pharmacology June 2003, 63 (6) 1382-1388; DOI: https://doi.org/10.1124/mol.63.6.1382
Hanlin Gao
Departments of Radiology (H.G., E.F.Y., R.M.S.) and Molecular Virology, Immunology, and Medical Genetics (H.G., R.M.S.), College of Medicine and Public Health and College of Pharmacy (K.K.C.), the Ohio State University, Columbus, Ohio; and Abbott Laboratories (L.L.S.), Abbott Park, Illinois
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Edith F. Yamasaki
Departments of Radiology (H.G., E.F.Y., R.M.S.) and Molecular Virology, Immunology, and Medical Genetics (H.G., R.M.S.), College of Medicine and Public Health and College of Pharmacy (K.K.C.), the Ohio State University, Columbus, Ohio; and Abbott Laboratories (L.L.S.), Abbott Park, Illinois
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kenneth K. Chan
Departments of Radiology (H.G., E.F.Y., R.M.S.) and Molecular Virology, Immunology, and Medical Genetics (H.G., R.M.S.), College of Medicine and Public Health and College of Pharmacy (K.K.C.), the Ohio State University, Columbus, Ohio; and Abbott Laboratories (L.L.S.), Abbott Park, Illinois
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Linus L. Shen
Departments of Radiology (H.G., E.F.Y., R.M.S.) and Molecular Virology, Immunology, and Medical Genetics (H.G., R.M.S.), College of Medicine and Public Health and College of Pharmacy (K.K.C.), the Ohio State University, Columbus, Ohio; and Abbott Laboratories (L.L.S.), Abbott Park, Illinois
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Robert M. Snapka
Departments of Radiology (H.G., E.F.Y., R.M.S.) and Molecular Virology, Immunology, and Medical Genetics (H.G., R.M.S.), College of Medicine and Public Health and College of Pharmacy (K.K.C.), the Ohio State University, Columbus, Ohio; and Abbott Laboratories (L.L.S.), Abbott Park, Illinois
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

The two known antineoplastic quinoxaline topoisomerase II poisons, XK469 (NSC 697887) and CQS (chloroquinoxaline sulfonamide, NSC 339004), were compared for DNA cleavage site specificity, using purified human topoisomerase IIα and human topoisomerase IIβ. The DNA cleavage intensity pattern for topoisomerase IIα poisoning by CQS closely resembled that of VM-26, despite the lack of any apparent common pharmacophore. In contrast, the topoisomerase IIα DNA cleavage intensity patterns of XK469 and CQS were very different from one another despite the similar overall structures of the two drugs. This suggests that the differences in DNA site specificity of topoisomerase II poisoning by XK469 and CQS may be caused by differences in their geometry, side chains, or electronic structure. The topoisomerase IIβ-mediated DNA cleavage sites of CQS and XK469 were also very different from one another, adding further support to this idea. Earlier work has demonstrated that a number of specific topoisomerase II poisons show very similar patterns of DNA cleavage with either topoisomerase IIα or topoisomerase IIβ, suggesting that the topoisomerase II isozymes play only a minor role in choices of DNA cleavage sites. However, both of the quinoxaline topoisomerase II poisons in this study showed distinctly different and unique DNA cleavage intensity patterns with each topoisomerase II isozyme. This indicates that topoisomerase II isozymes can play a major role in DNA cleavage site selection for some classes of topoisomerase II poisons.

  • Received November 8, 2002.
  • Accepted March 10, 2003.
  • The American Society for Pharmacology and Experimental Therapeutics
View Full Text

MolPharm articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Molecular Pharmacology: 63 (6)
Molecular Pharmacology
Vol. 63, Issue 6
1 Jun 2003
  • Table of Contents
  • About the Cover
  • Index by author
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Molecular Pharmacology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
DNA Sequence Specificity for Topoisomerase II Poisoning by the Quinoxaline Anticancer Drugs XK469 and CQS
(Your Name) has forwarded a page to you from Molecular Pharmacology
(Your Name) thought you would be interested in this article in Molecular Pharmacology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleArticle

DNA Sequence Specificity for Topoisomerase II Poisoning by the Quinoxaline Anticancer Drugs XK469 and CQS

Hanlin Gao, Edith F. Yamasaki, Kenneth K. Chan, Linus L. Shen and Robert M. Snapka
Molecular Pharmacology June 1, 2003, 63 (6) 1382-1388; DOI: https://doi.org/10.1124/mol.63.6.1382

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Research ArticleArticle

DNA Sequence Specificity for Topoisomerase II Poisoning by the Quinoxaline Anticancer Drugs XK469 and CQS

Hanlin Gao, Edith F. Yamasaki, Kenneth K. Chan, Linus L. Shen and Robert M. Snapka
Molecular Pharmacology June 1, 2003, 63 (6) 1382-1388; DOI: https://doi.org/10.1124/mol.63.6.1382
del.icio.us logo Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Materials and Methods
    • Results
    • Discussion
    • Acknowledgments
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • The binding site for KCI807 in the androgen receptor
  • Fatty acid amide hydrolase in cisplatin nephrotoxicity
  • eCB Signaling System in hiPSC-Derived Neuronal Cultures
Show more Article

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About Molecular Pharmacology
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Drug Metabolism and Disposition
  • Journal of Pharmacology and Experimental Therapeutics
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-0111 (Online)

Copyright © 2023 by the American Society for Pharmacology and Experimental Therapeutics